Measuring method and device of fluorescence lifetime

A technology of fluorescence lifetime and measurement method, which is applied in the direction of measuring devices, fluorescence/phosphorescence, and material analysis through optical means, which can solve the problems of low photon counting efficiency, avoid long time-consuming, and avoid low resolution of fluorescence lifetime , Improve the effect of measurement speed

Active Publication Date: 2018-09-04
SHENZHEN UNIV
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Problems solved by technology

This means that the photon counting efficiency of TCSPC is very low, and it takes a long time to count to meet the measurement accuracy requirements of the decay curve

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  • Measuring method and device of fluorescence lifetime
  • Measuring method and device of fluorescence lifetime
  • Measuring method and device of fluorescence lifetime

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[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] Because there is the technical problem of slow measurement speed when using TCSPC (time gating) method to measure fluorescence lifetime in the prior art.

[0025] In order to solve the above technical problems, the present invention proposes a method and device for measuring fluorescence lifetime, using gating technology to realize the filtering of photons in a short period of time under high excitation power "stacking effect" of photons, and using compressed sensing technology to achieve low sampling High sampling rate signal acquisition is used to reconstruct the source signal with high sa...

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Abstract

The invention is applicable to the technical field of photoelectric detection, and provides a measuring method and a measuring device of fluorescence lifetime. The measuring method comprises the following steps: firstly, using each row of a preset measurement matrix as a sampling mode, using a non-zero value position in each sampling mode as a sparse signal channel for time gate controlled delayedsampling, performing delayed sampling on a sample in each sampling mode, accumulating the number of photons in the sparse signal channel in the current sampling mode to obtain a compressed sensing measurement value, and repeating each sampling mode to obtain a series of compressed sensing measurement values; then, by virtue of the compressed sensing measurement values and through combination of the measurement matrix, solving an equation under an l1 norm optimization algorithm to obtain a sparse coefficient; finally, acting on the sparse coefficient through known reverse sparse matrix transformation, and performing signal reconstruction to obtain a fluorescence lifetime attenuation curve so as to achieve fluorescence lifetime measurement. By the measuring method provided by the invention,the measurement speed of the fluorescence lifetime is increased.

Description

technical field [0001] The invention belongs to the field of photoelectric detection, in particular to a method and device for measuring fluorescence lifetime. Background technique [0002] The modern methods of fluorescence lifetime measurement mainly include: Strobe Techniques, Phase Modulation Methods, Streak Cameras, Upconversion Methods and Time-correlated single-photon counting methods (Time -Correlated Single-Photon Counting, TCSPC). [0003] Among them, single photon counting is a powerful means to detect extremely weak light. This technology digitally extracts the optical signal from the thermal noise by distinguishing the photoelectron pulse excited by a single photon in the detector (PMT). An important component of TCSPC technology is the time-amplitude converter (TAC), which is used to record the time interval of two different electrical signals. When the excitation light source emits a beam of short pulse light, the detector PMT converts the light signal into ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6408
Inventor 陈秉灵屈军乐严伟林丹樱
Owner SHENZHEN UNIV
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