Method and system for measuring fluorescence service life

A fluorescence lifetime and measurement method technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of low efficiency and slow fluorescence lifetime measurement, and achieve the effect of high efficiency and fast acquisition

Inactive Publication Date: 2010-09-15
SHENZHEN UNIV
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Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a fluorescence lifetime measurement method, which aims to solve the problems of slow and low efficiency of the existing fluorescence lifetime measurement

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  • Method and system for measuring fluorescence service life
  • Method and system for measuring fluorescence service life
  • Method and system for measuring fluorescence service life

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Embodiment Construction

[0028] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0029] In the embodiment of the present invention, the excitation light is divided into multiple excitation photon beams that correspond to multiple sub-regions on the sample one by one, and the excitation photon beams scan the corresponding measurement sub-regions to form multi-line parallel excitation fluorescence, so as to quickly obtain the fluorescence lifetime. Purpose.

[0030] The fluorescence lifetime measurement method provided by the embodiment of the present invention comprises the following steps:

[0031] generate excitation light;

[0032] dividing the excitation light into a plura...

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Abstract

The invention is suitable for the field of photoelectric detection and provides a method and a system for measuring fluorescence service life. The method for measuring fluorescence service life comprises the following steps of: generating exciting light; splitting the exciting light into a plurality of sub-beams corresponding to a plurality of sub-regions of a sample; simultaneously scanning the sub-regions by utilizing the sub-beams so that a fluorescent substance in each sub-region emits fluorescence; collecting the fluorescence emitted during scanning in real time; and distinguishing the service life information of the fluorescence. In the embodiment of the invention, the exciting light is split into a plurality of exciting light sub-beams corresponding to a plurality of sub-regions on the sample one by one and the corresponding measuring sub-regions are scanned by utilizing the exciting light sub-beams to form multiline parallel exciting fluorescence, thereby achieving the aims of rapidly acquiring the fluorescence service life and having high efficiency.

Description

technical field [0001] The invention belongs to the field of photoelectric detection, in particular to a fluorescence lifetime measurement method and system. Background technique [0002] Fluorescence microscopy has become an important tool in life sciences, especially cell biology research. Two-photon excitation fluorescence microscopy has the advantages of small killing effect on living organisms, large penetration depth, and chromatographic ability, and has become an important means of life science research. In addition to the distribution and change of fluorescence intensity, the fluorescence lifetime is very sensitive to the microenvironment where the fluorophore is located, and can be affected by ion concentration (Ca 2+ , Na + ), pH and pO 2 Therefore, two-photon excited fluorescence lifetime measurement can provide functional information with high spatiotemporal resolution for biomedical detection and analysis, and is expected to be used in the study of protein-pr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 邵永红屈军乐牛憨笨
Owner SHENZHEN UNIV
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