Laser scanning saturate structured light illumination microscopic method and device based on phase modulation
A technology of structured light illumination and laser scanning, applied in the field of confocal microscopy, can solve problems such as photobleaching and damage to fixed samples, and achieve the effects of easy operation, reduced requirements, and reduced light intensity
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Embodiment 1
[0058] Such as figure 1 As shown, it is a phase-modulated laser scanning saturation structured illumination microdevice modulated by a spatial light modulator, including a laser 1, a collimator lens 2, a polarizer 3, a spatial light modulator 4a, and a half-wave plate 5. Scanning galvanometer 6 in x direction, 7 galvanometer scanning in y direction, scanning lens 8, field lens 9, dichromatic mirror 10, objective lens 11, sample 12, stage 13, lens 14, photodetector 15, computer 16 and spatial light modulator control box 17.
[0059] use figure 1 The shown device implements phase modulation-based laser scanning saturated structured illumination microscopy for fluorescent samples, and the process is as follows:
[0060] (1) Laser 1 emits illumination light, which is collimated by collimating lens 2;
[0061] (2) The collimated illumination light is polarized into linearly polarized light by the polarizer 3;
[0062] (3) The polarized illumination light passes through the sp...
Embodiment 2
[0066] Such as image 3 As shown, it is a phase-modulated laser scanning saturation structured illumination microdevice modulated by a 0-π phase plate, including a laser 1, a collimator lens 2, a polarizer 3, and a 0-π phase plate 4b, Half-wave plate 5, scanning vibrating mirror 6 in x direction, scanning vibrating mirror 7 in y direction, scanning lens 8, field mirror 9, dichromatic mirror 10, objective lens 11, sample 12, stage 13, lens 14, photodetector 15 and computer 16 .
[0067] use image 3 The shown device implements phase modulation-based laser scanning saturated structured illumination microscopy for fluorescent samples, and the process is as follows:
[0068] (1) Laser 1 emits illumination light, which is collimated by collimating lens 2;
[0069] (2) The collimated illumination light is polarized into linearly polarized light by the polarizer 3;
[0070] (3) The polarized illumination light passes through the 0-π phase plate 4b to load the 0-π phase, then pass...
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