Two-dimensional modulation technique-based method for acquiring shear-layer images

A modulation technology and image acquisition technology, which is applied in the field of slice image acquisition, can solve the problem of limited improvement of axial resolution and achieve the effect of high axial resolution

Active Publication Date: 2010-03-03
MOTIC CHINA GRP CO LTD
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Problems solved by technology

[0006] The present invention provides a slice image acquisition method based on two-dimensional modulation technology, the main purpose of which is to overcome the axial resolution caused by the phase shift algorithm based on one-dimensional modulation technology in the existing structured light microscope slice image acquisition method improve limited disadvantages

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  • Two-dimensional modulation technique-based method for acquiring shear-layer images

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Embodiment Construction

[0015] Specific embodiments of the present invention will be described below with reference to the accompanying drawings.

[0016] A structured light microscope imaging system based on a two-dimensional spatial modulator such as figure 1 As shown, the light emitted by the light source 1 is modulated by the two-dimensional spatial light modulator 2, then passed through the lens 3, reflected by the beam splitter 4 to the objective lens 5, and projected on the sample 6, and the light emitted from the sample passes through the imaging lens 7 After imaging on the detector of the digital camera 8; the digital camera 8 and the two-dimensional spatial light modulator 2 are connected to the computer 9 through the signal line, and the computer 9 controls the two-dimensional spatial light modulator 2 to generate modulation patterns, and the digital camera synchronously collects 8 acquired image data.

[0017] The spatial light modulator 2 is an array composed of a plurality of micro-spa...

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Abstract

The invention discloses a two-dimensional modulation technique-based method for acquiring shear-layer images. In the method, a two-dimensional light modulator, such as a digital micro lens DMD, a transmission LCD and a reflective silicon-base LCOS, instead of a fiber grating in a microscope with the conventional structure is used for two-dimensional light modulation and generating a group of modulated patterns in different phases, and the modulated patterns as well as a two-dimensional phase-shift algorithm are used to rebuild the shear-layer images. Experiments show that under a condition ofkeeping the signal-to-noise ratio of the images, the shear-layer images rebuilt by two-dimensional modulation technology have a higher axial resolution compared with that of the shear-layer images rebuilt by one-dimensional modulation technology.

Description

technical field [0001] The invention relates to a slice image acquisition method of a structured light microscope, in particular to a slice image acquisition method based on two-dimensional modulation technology. Background technique [0002] Structured light microscope is a kind of microscope that introduces structured light measurement method into confocal measurement. It adds the illumination modulation function on the basis of the fluorescence microscope, that is, a spatial light modulator such as a grating is inserted in the illumination optical path, and the illumination is modulated by the grating and then projected on the sample through the objective lens, and will be irradiated by the modulated illumination on the focal plane of the sample , while it is not modulated away from the focal plane, the modulated focal plane information and the non-modulated non-focus plane information will enter the imaging detector at the same time, change the modulation phase of the il...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00G06T7/00
Inventor 陈木旺陈进肖倩
Owner MOTIC CHINA GRP CO LTD
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