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TV constraint based tomography phase microscope reconstruction method

A phase microscope and tomography technology, which is applied in the field of biomedical image reconstruction, can solve the problems of difficult reconstruction of sample refractive index images, and achieve the effects of benefiting dynamic characteristics, reducing the number, and alleviating the effect of axial stretching

Inactive Publication Date: 2016-12-07
ZHEJIANG UNIV
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Problems solved by technology

Due to the lack of collected data, it is difficult to reconstruct the refractive index image of the sample using ordinary analytical algorithms, which poses a new challenge to image reconstruction

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  • TV constraint based tomography phase microscope reconstruction method
  • TV constraint based tomography phase microscope reconstruction method
  • TV constraint based tomography phase microscope reconstruction method

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Embodiment Construction

[0038] In order to describe the present invention more specifically, the technical solutions of the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0039]Such as figure 1 In the tomographic phase microscope system shown, the laser light emitted by the He-Ne laser is divided into two beams, the sample beam and the reference beam, through a beam splitter. At the sample beam, the direction of the beam is controlled by the scanning array mirror (GM), so that the beam rotates with a set angular step, and then the beam is focused by the lens (L1, f=250mm) on the oil immersion objective lens (CL, Nikon, 1.4 NA) on the back focal plane. The distance from the scanning array mirror to the lens L1 is equal to the distance from the lens L1 to the back focal plane (BF) of the oil immersion objective lens to ensure the conjugate of the scanning array mirror and the sample (Sample). The sample was placed in the s...

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Abstract

The invention discloses a TV constraint based tomography phase microscope reconstruction method. The method includes (1) acquiring a multi-angle phase image, (2) establishing a system matrix imaging model, (3) adding TV constraint, and (4) reconstructing a three-dimensional refractive index image. By establishing the system matrix model of microscope imaging, various uncertain factors in the data collection process are brought into a unified description framework; based on the image sparse feature, the image axial stretching effect due to missing cone is effectively alleviated, and the axial resolution of a microscope is improved; and meanwhile, the phase data is acquired by sparse angles, the total acquisition angle quantity is reduced, the imaging time is reduced, the time resolution of the microscope is improved, and the observation of the dynamic characteristic of biological samples is facilitated.

Description

technical field [0001] The invention belongs to the technical field of biomedical image reconstruction, and in particular relates to a tomographic phase microscope reconstruction method based on TV (Total Variation, total variation) constraints. Background technique [0002] Many living cells and biological samples exhibit a certain degree of transparency, and the refractive index itself is often an intrinsic source of contrast used to distinguish structural properties of cells or biological samples. In recent years, as light microscopy has continued to develop with higher spatial and temporal resolutions, it has become an indispensable tool in biological and medical research. Among them, tomographic phase microscopy (TPM) has increasingly become a research hotspot for biologists because it directly images the refractive index distribution of biological samples without adding any dyes. [0003] The tomographic phase microscope usually uses a heterodyne laser interferometer ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T17/00
CPCG06T17/00G06T2200/04G06T2207/10061G06T2207/10101G06T2210/41
Inventor 刘华锋郭敏
Owner ZHEJIANG UNIV
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