Spectrally encoded confocal and optical coherence tomography cooperative imaging method and system

Abstract

The invention discloses a spectrally encoded confocal and optical coherence tomography cooperative imaging method and system. The system is based on the combination of an OCT (Optical Coherence Tomography) imaging technique and an SECM (Spectrally Encoded Confocal Microscopy) technique, a sample arm of an OCT system and a sample arm of an SECM system share the same light path, so that light beams emitted from the sample arms of the OCT system and the SECM system simultaneously scan the same position of a sample, and light which is scattered and returns back from the sample at a single time is respectively transmitted to a detection arm through respective light paths. The detection arm adopts two optical fiber collimating lenses to irradiate an optical grating from different incident angles, so that the optical grating diffracts two groups of spectral signals which are emitted without crossing over, i.e. SECM and OCT signals. The light path of the sample arm in the system is simpler, the implementation is facilitated, the light path is more compact and stable, the system imaging quality is high and biological tissue structures can be imaged more accurately.

Description

technical field [0001] The present invention relates to spectral encoding confocal microscopy technology and optical coherence tomography imaging technology, in particular to a synchronous imaging technology of spectral encoding confocal and optical coherence tomography. Background technique [0002] Optical coherence tomography (OCT) technology is an emerging biomedical optical imaging technology, which can realize non-contact, non-destructive, high-resolution imaging of the structure and physiological functions of biological tissues. Early detection and in vivo biopsy have broad application prospects. [0003] Spectrally Encoded Confocal Microscopy (SECM) technology is a confocal imaging method of micro-invasive single-mode fiber, which uses a broadband light source and a diffraction grating to simultaneously detect the reflectance at multiple lateral positions of the sample. By detecting the spatial information of the sample arm, the light-encoded confocal microscopy sys...

AI Technical Summary

Problems solved by technology

[0005] Most of the currently proposed collaborative imaging methods for the OCT system and the SECM system are based on the swing of the scanning galvanometer to switch between the two systems. The structure of the system using the galvanometer as a mechanical switch is relatively complicated, and there is no To achieve real simultaneous scanning and imaging, if the sample to be measured is a living sample, it is very likely that the imaging positions of the two systems will not be aligned due to the movement of the living body during the switching process

In addition, most of the currently proposed collaborative imaging methods for the OCT system and the SECM system use two light sources for the OCT system and the SECM system respectively, and also use two detection arms to detect the sample signal of the OCT system and the SECM system respectively. sample signal, which makes the integrated system too complicated, not stable enough, and the construction cost is high

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