Light slice fluorescence microscopic imaging method and device based on relocation
A microscopic imaging and repositioning technology, applied in the field of optical imaging, can solve the problems of limiting the range of imaging field of view, reducing lateral resolution, increasing sample photobleaching, etc., without reducing the field of view, improving axial resolution, The effect of not reducing the imaging speed
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Embodiment 1
[0048] see figure 2 , the repositioning-based optical section fluorescence microscopic imaging device of the present embodiment includes a laser 1, a single-mode optical fiber 2, a collimating lens 3, a telescope composed of a convex lens 4 and a convex lens 5, a cylindrical lens 6, and a first relay lens 7. Mirror 8, second relay lens 9, third relay lens 10, illumination objective lens 11, fluorescent sample 12, first detection objective lens 13, first optical filter 14, first tube lens 15, first CCD Camera 16, second detection objective lens 17, second optical filter 18, half mirror 19, second tube lens 20, second CCD camera 21, third tube lens 22, third CCD camera 23, computer 24.
[0049] use figure 2 With the imaging device shown, the specific steps for implementing the repositioning-based optical sectioning fluorescence microscopy imaging method are as follows:
[0050] (1) The laser light emitted by the laser 1 is filtered by the single-mode fiber 2, and then the la...
Embodiment 2
[0064] see Figure 4 , the repositioning-based optical section fluorescence microscopic imaging device of the present embodiment includes a laser 1, a single-mode optical fiber 2, a collimating lens 3, a telescope composed of a convex lens 4 and a convex lens 5, a cylindrical lens 6, and a first relay lens 7. Mirror 8, second relay lens 9, third relay lens 10, illumination objective lens 11, fluorescent sample 12, first detection objective lens 13, first optical filter 14, first tube lens 15, first CCD Camera 16, second detection objective lens 17, half-transparent mirror 25, vibrating mirror 26, pinhole I27, photoelectric tube I28, pinhole II29, photoelectric tube II30, computer 24.
[0065] use Figure 4 The specific process of implementing the repositioning-based optical sectioning fluorescence microscopy imaging method for the imaging device shown is as follows:
[0066] (1) The laser light emitted by the laser 1 is filtered by the single-mode fiber 2, and then the laser b...
Embodiment 3
[0078] The repositioning-based optical section fluorescence microscopy imaging method provided in this embodiment has been included in Embodiment 1 or Embodiment 2, and will not be repeated here.
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