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Preparation method and application of egg shell membrane composite nano-silver thin film used for artificial skin

An artificial skin and egg membrane technology, applied in medical science, bandages, prostheses, etc., can solve the problems of complex operation of electrospinning technology, time-consuming reduction reagents, environmental pollution, etc., achieving low cost, less time-consuming, The effect of simple operation process

Active Publication Date: 2017-01-11
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation of electrospinning technology is relatively complicated and the cost is high, and the commonly used reducing reagents take more time and are prone to produce environmental pollutants

Method used

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  • Preparation method and application of egg shell membrane composite nano-silver thin film used for artificial skin
  • Preparation method and application of egg shell membrane composite nano-silver thin film used for artificial skin
  • Preparation method and application of egg shell membrane composite nano-silver thin film used for artificial skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: a kind of preparation method of the egg membrane composite nano-silver thin film that is used for artificial skin, comprises the steps:

[0036] (1) Take a fresh egg, break it lightly, discard the egg white and yolk, wash the egg shell in deionized water, then carefully separate the egg membrane with tweezers, wash it again with deionized water, and cut the egg membrane into 2cm ×2cm size;

[0037] (2) Place the egg membrane prepared in step (1) in a hydrochloric acid solution, soak it at room temperature for 3 days, and then wash it with deionized water for 3 times, each time for 5 minutes. The concentration of the hydrochloric acid solution is 1mM, and the pH= 3;

[0038] (3) Dissolving dopamine into Tris-HCl solution, the concentration of the Tris-HCl solution is 10mM, the concentration of the prepared dopamine solution is 2mg / ml, and the pH is adjusted to 8.5;

[0039] (4) Place the egg membrane prepared in step (2) in the dopamine solution prepared ...

Embodiment 2

[0041] Embodiment 2: a kind of preparation method of egg membrane composite nano-silver film for artificial skin comprises the following steps:

[0042] (1) Take a fresh egg, break it lightly, discard the egg white and yolk, wash the egg shell in deionized water, then carefully separate the egg membrane with tweezers, wash it again with deionized water, and cut the egg membrane into 1cm ×3cm size;

[0043] (2) Place the egg membrane prepared in step (1) in hydrochloric acid solution, soak it at room temperature for 1 day, and then wash it with deionized water for 3 times, each time for 5 minutes. The concentration of the hydrochloric acid solution is 10mM, and the pH= 1;

[0044] (3) Dissolving dopamine into Tris-HCl solution, the concentration of the Tris-HCl solution is 30mM, the concentration of the prepared dopamine solution is 2mg / ml, and the pH is adjusted to 9.0;

[0045] (4) Put the egg membrane prepared in step (2) into the dopamine solution prepared in step (3), so...

Embodiment 3

[0047] Embodiment 3: a kind of preparation method of egg membrane composite nano-silver thin film for artificial skin, comprises the steps:

[0048] (1) Take a fresh egg, break it lightly, discard the egg white and yolk, wash the egg shell in deionized water, then carefully separate the egg membrane with tweezers, wash it again with deionized water, and cut the egg membrane into 3cm ×3cm size;

[0049] (2) Place the egg membrane prepared in step (1) in a hydrochloric acid solution, soak it at room temperature for 4 days, and then wash it with deionized water for 5 times, each time for 3 minutes. The concentration of the hydrochloric acid solution is 100mM, and the pH= 3;

[0050] (3) Dissolving dopamine into Tris-HCl solution, the concentration of the Tris-HCl solution is 100mM, the concentration of the prepared dopamine solution is 4mg / ml, and the pH is adjusted to 9.5;

[0051] (4) Put the egg membrane prepared in step (2) into the dopamine solution prepared in step (3), s...

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Abstract

The invention discloses a preparation method and application of an egg shell membrane composite nano-silver thin film used for an artificial skin. The preparation method comprises the following steps: removing egg white and yolks of fresh eggs and washing egg shells with de-ionized water; separating egg shell membranes and washing the egg shell membranes with the de-ionized water; cutting the egg shell membranes into sizes of (1cm-3cm)*(1cm-3cm) and putting the egg shell membranes into a hydrochloric acid solution with the concentration of 1mM to 100mM and the pH (Potential of Hydrogen) of 1 to 3; immersing for 1 to 4 days at room temperature and washing with the de-ionized water; then putting the egg shell membranes into a dopamine solution with the concentration of 2mg / mL to 4mg / mL and the pH of 8.5 to 9.5; immersing for 6h to 20h at room temperature and washing with the de-ionized water; then putting the egg shell membranes into a silver nitrate solution with the concentration of 10nM to 50nM; immersing for 6h to 20h at room temperature without light, and then washing with the de-ionized water; and naturally drying at room temperature to obtain the egg shell membrane composite nano-silver thin film used for the artificial skin. The prepared egg shell membrane composite nano-silver thin film is used for manufacturing the artificial skin, has excellent mold resistance, and has no obvious biological toxicity; and the preparation method is simple in process, less in consumed time and low in cost.

Description

technical field [0001] The invention belongs to the technical field of material application, and in particular relates to a preparation method and application of an egg membrane composite nano-silver thin film for artificial skin. Background technique [0002] As the largest organ of the human body, the skin can protect internal organs from damage and maintain water and electrolyte balance. Skin defects caused by burns, trauma, diabetic foot ulcers, etc. will lead to serious infection, water and electrolyte imbalance, scar hyperplasia and other problems in the body, which will bring huge mental and physical pain and heavy medical burden to patients. Therefore, restoring the barrier function of the skin has become a top priority. However, some patients with large skin defects, such as patients with extensive burns, cannot effectively seal the wound in time due to their limited skin source. In order to solve this problem, it is very necessary to apply a suitable artificial s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/54A61L27/36A61L15/40A61L15/46
CPCA61L15/40A61L15/46A61L27/3604A61L27/54A61L27/60A61L2300/104A61L2300/404A61L2300/80
Inventor 刘梦龙邢孟秋吴军罗高兴贺伟峰徐瑞王玉振王颖
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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