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Dissolving and filming method of egg shell membrane

A technology of egg shell membrane and film forming method, which is applied in the field of dissolution and film formation of egg shell membrane, can solve the problems of expensive, difficult to remove, unfavorable application of biological materials, etc., and achieve the effect of low cost and cost reduction

Inactive Publication Date: 2003-07-30
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high concentration of highly toxic and foul-smelling β-thiopropionic acid solvent used in the preparation process, it is not only expensive, but also very difficult to remove from egg shell membrane products
Therefore, it is not conducive to the application in biomaterials

Method used

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  • Dissolving and filming method of egg shell membrane
  • Dissolving and filming method of egg shell membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Prepare an aqueous solution (co-solvent) containing a molar concentration of 1.25β-thiopropionic acid and a concentration of 10% 7-acid by mass percentage, add 60 grams of dried egg shell membranes to 2 liters of co-solvent, and lower the temperature while stirring Rise to 80°C and keep it. After 20.5 hours, it is found that the egg shell membrane is almost completely dissolved. When the solution is removed from the heater and cooled to room temperature, it is separated by a centrifuge and a small amount of precipitated substances are removed. Take a certain amount The separation solution was poured on a glass plate placed horizontally. After several hours, when the transparent separation solution had gradually formed a film and no longer had fluidity, it was washed with a certain amount of methanol and neutralized with PBS buffer. to neutrality, then washed with deionized water, dried and peeled off to obtain a planar egg shell membrane, which does not have the stench o...

example 2～ example 10

[0017] The preparation method and process are the same as in Example 1, except that the amount of raw egg shell membrane (ESM), the concentration of β-thiopropionic acid, the type and concentration of organic acid, and the dissolution temperature have been changed accordingly. The respective conditions and The obtained results are listed in Table 1.

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Abstract

The present invention belongs to the field of biological material preparing technology. Egg shell membrane is first dissolved completely in water solution of beta-thiopropionic acid and organic acid as cosolvent through heating to 80-95 deg.c and stirring; after centrifugal separatino and elimination of precipitate, the separated liquid is cast directly onto glass plate or steel plate and dried to form film; and the film is washed with methanol, neutralized with buffering phosphate solution, washed with deionized water, dried and peeled to obtain planar egg shell film. Without residual toxic solvent and crosslinking agent used, the egg shell film can be used widely.

Description

technical field [0001] The invention relates to a method for dissolving and forming egg shell membranes, and belongs to the technical field of biological material preparation. Background technique [0002] As a by-product of human life, egg shells exist in large quantities, and its inner membrane—egg shell membrane (ESM) is mainly composed of various proteins such as collagen (type I, V, X), osteopontin, salivary protein, etc., and a small amount of polysaccharides , has a wide range of potential applications and values ​​in heavy metal ion selective adsorption materials, artificial skin or dressing materials for skin treatment, enzyme immobilization carriers, cell adhesion growth matrix or drug controllable slow release materials, etc. However, the curved shape of egg shell membranes and the insoluble nature of containing a large number of cross-links limit the application of egg shell membranes. Therefore, it is urgently needed and very meaningful to find a method that ca...

Claims

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Application Information

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IPC IPC(8): A61K47/46A61L15/40A61L27/06B01J20/22C08J5/18C12N11/02
Inventor 于建弋峰郭朝霞李强
Owner TSINGHUA UNIV
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