Dissolving and filming method of egg shell membrane

A technology of egg shell membrane and film forming method, which is applied in the field of dissolution and film formation of egg shell membrane, can solve the problems of expensive, difficult to remove, unfavorable application of biological materials, etc., and achieve the effect of low cost and cost reduction
CN1432592AInactive Publication Date: 2003-07-30TSINGHUA UNIV

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2003-07-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the field of biological material preparing technology. Egg shell membrane is first dissolved completely in water solution of beta-thiopropionic acid and organic acid as cosolvent through heating to 80-95 deg.c and stirring; after centrifugal separatino and elimination of precipitate, the separated liquid is cast directly onto glass plate or steel plate and dried to form film; and the film is washed with methanol, neutralized with buffering phosphate solution, washed with deionized water, dried and peeled to obtain planar egg shell film. Without residual toxic solvent and crosslinking agent used, the egg shell film can be used widely.
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Description

technical field

[0001] The invention relates to a method for dissolving and forming egg shell membranes, and belongs to the technical field of biological material preparation. Background technique

[0002] As a by-product of human life, egg shells exist in large quantities, and its inner membraneegg shell membrane (ESM) is mainly composed of various proteins such as collagen (type I, V, X), osteopontin, salivary protein, etc., and a small amount of polysaccharides , has a wide range of potential applications and values ​​in heavy metal ion selective adsorption materials, artificial skin or dressing materials for skin treatment, enzyme immobilization carriers, cell adhesion growth matrix or drug controllable slow release materials, etc. However, the curved shape of egg shell membranes and the insoluble nature of containing a large number of cross-links limit the application of egg shell membranes. Therefore, it is urgently needed and very meaningful to find a method that ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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