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Production method and application of egg-shell membrane collagen peptides

A production method, membrane collagen technology, applied in the field of peptides, can solve problems such as product quality control, operability and energy consumption, and achieve the effect of simple operation steps and conditions, and low energy consumption

Inactive Publication Date: 2012-06-27
淮安鸿玛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to being limited by the performance of raw materials, the existing technology has problems in product quality control, operability, energy consumption, especially the realization of large-scale, environmentally friendly production, etc.

Method used

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  • Production method and application of egg-shell membrane collagen peptides
  • Production method and application of egg-shell membrane collagen peptides
  • Production method and application of egg-shell membrane collagen peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Take 1000 grams of fresh egg shells, wash them with water to remove foreign matter, crush the shells to remove the egg shells, and then prepare the protease solution (200ml, concentration 800U / L) with egg membranes containing a small amount of shells, set the temperature at 60°C , after stirring for 2 hours, slowly add NaOH solution, so that the mass concentration of NaOH in the mixture is 3%, and the temperature is 65°C, stir for 3 hours, and let stand. Take the supernatant, adjust the pH value to 7 with dilute hydrochloric acid to obtain a membrane protein solution, the volume of the membrane protein solution is 3 times of the original supernatant. Use 1k membrane equipment to desalt and concentrate, and spray dry at a temperature below 180°C to obtain egg membrane collagen polypeptide product A (about 37 grams).

Embodiment 2

[0038] Take 1000 grams of fresh egg shells, rinse with water to remove foreign matter, crush the shells to remove the egg shells, and then prepare the protease solution (200ml, concentration 1000U / L) with egg membranes containing a small amount of shells, set the temperature at 35°C , after stirring for 3 hours, slowly add NaOH solution, so that the mass concentration of NaOH in the mixture is 3%, and the temperature is 50°C, stir for 4 hours, and let stand. Take the supernatant, adjust the pH value to 9 with dilute hydrochloric acid to obtain a membrane protein solution, the volume of the membrane protein solution is 3 times of the original supernatant. Use a membrane with a membrane pore size of 300k to filter the concentrated membrane protein solution, add pure water to elute the salt in the solution to bring the pH value back to 7, and recover the concentrated solution and filtrate. The concentrated solution is spray-dried or freeze-dried at a temperature below 180° C. to ...

Embodiment 3

[0040] The filtrate obtained by preparing product B in Example 2 was separated by a 30k membrane, and the intercepted liquid and the secondary filtrate were recovered. The resulting intercepted solution was spray-dried at a temperature below 180° C. to obtain product C (about 15 grams) of the egg membrane collagen polypeptide.

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Abstract

The invention discloses a production method and application of egg-shell membrane collagen peptides. The method comprises the following steps of: (1) taking an egg membrane out of an egg shell, and smashing to over 40 meshes; (2) adding egg membrane powder obtained in the step (1)into a papain solution with the concentration of 800-1,000 U / L, and stirring at the temperature of 35-50 DEG C for 2-3 hours to obtain a mixed material, wherein the solid-liquid ratio is 1:(2-5); (3) adding an NaOH solution into the mixed material obtained in the step (2) till the mass concentration of NaOH is 2-4.5 percent, stirring at the temperature of 45-85 DEG C for 3-4 hours, standing, and taking a supernatant; and (4) adjusting the pH value of the supernatant obtained in the step (3) to 7-11 with dilute hydrochloric acid to obtain an egg-membrane collagen peptide liquid, desalting, concentrating and drying to obtain egg-shell membrane collagen peptides. The production method has simple operation steps and simple conditions, egg-shell membrane collagen peptides can be produced on a large scale, energy consumption is low, environmental pollution is avoided simultaneously, and two or more polypeptide surfactants can be produced simultaneously; and by changing operation conditions, the performance of the polypeptide surfactant can be adjusted and controlled.

Description

technical field [0001] The invention relates to the technical field of polypeptides, in particular to a production method and application of egg membrane collagen polypeptides. Background technique [0002] As a by-product of human life, egg shells exist in large quantities, and the egg shell membranes attached to them are particularly likely to cause great pollution to the environment. Egg membrane contains a variety of proteins (about 90%) such as: collagen (type I, V, X), osteopontin, albumin, salivary protein, etc., and also contains chondroitin sulfate, hyaluronic acid, N-acetyl Glucosamine galactose, glucuronic acid, etc., are mainly used to treat and relieve joint pain, etc., and are high-quality raw materials for beauty, health care products, cosmetics, and daily chemical washing. Egg membrane proteins have broad application prospects in selective adsorption materials for heavy metal ions, artificial skin or dressing materials for skin therapy, enzyme immobilization...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K8/65A61Q11/00A61Q15/00A61Q19/00A61Q19/08A61Q19/10
Inventor 吕军仁
Owner 淮安鸿玛生物科技有限公司
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