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Biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, preparation method and application

A technology of biomimetic mineralization and eggshell membrane, which is applied in the field of medical biomimetic materials, can solve the problems of long preparation cycle and slow mineralization effect, and achieve the effects of improving mineralization efficiency, reducing mineralization time, and promoting mineral formation

Active Publication Date: 2020-06-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported that natural eggshell membranes can be used as templates to construct eggshell membrane / hydroxyapatite composites, there are disadvantages such as long preparation period and slow mineralization effect, and the preparation process needs to be further improved.

Method used

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  • Biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, preparation method and application
  • Biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, preparation method and application
  • Biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, preparation method and application

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Effect test

Embodiment 1

[0032] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,

[0033] S1, material preparation

[0034] To prepare the eggshell membrane, remove the egg white and yolk from the fresh egg, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 17% (v / v) citric acid solution for 50 minutes to obtain the outer eggshell membrane. layer eggshell membrane, the obtained outer eggshell membrane is cleaned with deionized water, cut to a suitable size, and dried for later use;

[0035] To prepare the enzymatic solution, dissolve in 1L deionized water for every 3g of pepsin and 5g of L-cysteine, and adjust the pH to 8 with 0.1M hydrochloric acid;

[0036]To prepare protein mineralization solution, every 0.3 g of phosvitin was dissolved in 1 L of 1 times simulated body fluid to obtain protein mineralization solution;

[0037] S2. ...

Embodiment 2

[0040] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,

[0041] S1, material preparation:

[0042] To prepare the eggshell membrane, remove the egg white and yolk from the fresh egg, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 17% (v / v) malic acid solution for 50 minutes to obtain the outer shell membrane. Layer eggshell membrane, clean the obtained inner layer eggshell membrane with deionized water, cut it to a suitable size, and dry it for later use;

[0043] To prepare the enzymatic solution, dissolve in 1L of deionized water for every 3g of keratinase and 5g of sodium sulfide, and adjust the pH to 6 with 0.1M hydrochloric acid;

[0044] Prepare a protein mineralization solution, dissolve every 0.3 g of phosvitin in 1 L of 1.5 times simulated body fluid to obtain a protein mineralization solution;

...

Embodiment 3

[0048] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,

[0049] S1, material preparation:

[0050] To prepare the eggshell membrane, remove the egg white and yolk from the fresh eggs, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 15% (v / v) acetic acid solution for 45 minutes to obtain the outer layer For the eggshell membrane, clean the obtained inner layer eggshell membrane with deionized water, cut it to a suitable size, and dry it for later use;

[0051] To prepare the enzymatic solution, dissolve 3 g of alkaline protease and 5 g of sodium sulfite in 1 L of deionized water, and adjust the pH to 8 with 0.1 M hydrochloric acid;

[0052] Prepare a protein mineralization solution, dissolve every 0.1 g of phosvitin in 1 L of 10 times simulated body fluid to obtain a protein mineralization solution;

[0053...

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Abstract

The invention belongs to the technical field of medical biomimetic materials, and particularly relates to a biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, a preparation method and application. The preparation method comprises the following steps of: S1, material preparation, including three parts of preparing an eggshell membrane, preparing enzymatic hydrolysate and preparing protein mineralized liquid, wherein the enzymatic hydrolysate is prepared by dissolving protease and a reducing agent in deionized water and adjusting the pH to 6-9, andthe protein mineralized liquid is prepared by dissolving water-soluble protein in mineralized liquid; and S2, preparation of the biomimetic mineralized membrane, namely soaking the inner eggshell membrane and / or the outer eggshell membrane in the enzymatic hydrolysate to obtain a modified eggshell membrane; and placing the modified eggshell membrane in the protein mineralized liquid for treatmentto obtain the biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction. The biomimetic mineralized membrane can be used as a bone tissue repair material, andthe preparation process has the characteristics of high mineralization efficiency, short preparation cycle time and good mineralization effect.

Description

technical field [0001] The invention belongs to the technical field of medical bionic materials, in particular to a biomimetic mineralization membrane prepared based on layered eggshell membrane and protein induction, a preparation method and an application. Background technique [0002] Bone defects are generally caused by genetic diseases, traffic accidents, pathological fractures, infections, etc. The repair of bone defects is a common problem in clinical practice, and tens of thousands of patients with bone injuries need treatment every year. [0003] Typically, the treatment of bone defects involves the use of autologous or allogeneic bone grafts. However, autologous bone grafts cause pain and complications at the donor site, and its size and shape are not easy to control; allograft bone grafts have limited sources, unpredictable healing ability, and low osteogenic ability, which cannot meet actual needs. Finding materials with good biocompatibility that can promote bo...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/46
CPCA61L27/227A61L27/3604A61L27/365A61L27/3687A61L27/3691A61L27/46A61L2430/02
Inventor 黄茜赵前程马美湖金永国付星
Owner HUAZHONG AGRI UNIV
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