Biomimetic mineralized membrane prepared based on layered eggshell membrane and protein induction, preparation method and application
A technology of biomimetic mineralization and eggshell membrane, which is applied in the field of medical biomimetic materials, can solve the problems of long preparation cycle and slow mineralization effect, and achieve the effects of improving mineralization efficiency, reducing mineralization time, and promoting mineral formation
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Embodiment 1
[0032] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,
[0033] S1, material preparation
[0034] To prepare the eggshell membrane, remove the egg white and yolk from the fresh egg, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 17% (v / v) citric acid solution for 50 minutes to obtain the outer eggshell membrane. layer eggshell membrane, the obtained outer eggshell membrane is cleaned with deionized water, cut to a suitable size, and dried for later use;
[0035] To prepare the enzymatic solution, dissolve in 1L deionized water for every 3g of pepsin and 5g of L-cysteine, and adjust the pH to 8 with 0.1M hydrochloric acid;
[0036]To prepare protein mineralization solution, every 0.3 g of phosvitin was dissolved in 1 L of 1 times simulated body fluid to obtain protein mineralization solution;
[0037] S2. ...
Embodiment 2
[0040] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,
[0041] S1, material preparation:
[0042] To prepare the eggshell membrane, remove the egg white and yolk from the fresh egg, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 17% (v / v) malic acid solution for 50 minutes to obtain the outer shell membrane. Layer eggshell membrane, clean the obtained inner layer eggshell membrane with deionized water, cut it to a suitable size, and dry it for later use;
[0043] To prepare the enzymatic solution, dissolve in 1L of deionized water for every 3g of keratinase and 5g of sodium sulfide, and adjust the pH to 6 with 0.1M hydrochloric acid;
[0044] Prepare a protein mineralization solution, dissolve every 0.3 g of phosvitin in 1 L of 1.5 times simulated body fluid to obtain a protein mineralization solution;
...
Embodiment 3
[0048] A method for preparing a biomimetic mineralized membrane based on layered eggshell membranes and protein induction, comprising the following steps,
[0049] S1, material preparation:
[0050] To prepare the eggshell membrane, remove the egg white and yolk from the fresh eggs, mechanically separate the inner eggshell membrane from the air chamber at the blunt end, and soak the remaining part in 15% (v / v) acetic acid solution for 45 minutes to obtain the outer layer For the eggshell membrane, clean the obtained inner layer eggshell membrane with deionized water, cut it to a suitable size, and dry it for later use;
[0051] To prepare the enzymatic solution, dissolve 3 g of alkaline protease and 5 g of sodium sulfite in 1 L of deionized water, and adjust the pH to 8 with 0.1 M hydrochloric acid;
[0052] Prepare a protein mineralization solution, dissolve every 0.1 g of phosvitin in 1 L of 10 times simulated body fluid to obtain a protein mineralization solution;
[0053...
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