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Method for preparing composite blood pressure lowering peptide by using marine organism

A blood pressure-lowering peptide, the technology of marine organisms, applied in the field of peptide preparation

Active Publication Date: 2012-04-11
山东好当家海森药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, at present, there are no research reports on the use of various marine organisms to prepare compound hypotensive peptides

Method used

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  • Method for preparing composite blood pressure lowering peptide by using marine organism
  • Method for preparing composite blood pressure lowering peptide by using marine organism

Examples

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Effect test

Embodiment 1

[0021] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0022] a. Selection and processing of raw materials Weigh 10kg of sea cucumber, 50kg of jellyfish, 25kg of kelp and 15kg of Chinese hairy shrimp as raw materials, soak in cold water for 36 hours, remove salt, wash, remove and drain, and use a beater respectively Perform beating treatment, then add 20 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0023] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) until the solution center temperature is 100° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0024] Adjust the temperature of the sea cucumber slurry to 55°C, add 90 g of compound prot...

Embodiment 2

[0033] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0034] a. Selection and processing of raw materials Weigh 5kg of sea cucumber, 65kg of jellyfish, 10kg of kelp and 20kg of Chinese hairy shrimp as raw materials, soak in cold water for 48 hours, remove salt, wash, remove and drain, and use a beater respectively Perform beating treatment, then add 10 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0035] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) to a solution center temperature of 95° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0036] Adjust the temperature of the sea cucumber slurry to 50°C and add 25 g of compound protease...

Embodiment 3

[0045] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0046] a. Selection and processing of raw materials Weigh 8kg of sea cucumber, 60kg of jellyfish, 22kg of kelp and 10kg of Chinese hairy shrimp as raw materials, soak them in cold water for 24 hours, remove the salt, wash them, remove and drain the water, and use a beater respectively Perform beating treatment, then add 30 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0047] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) until the solution center temperature is 100° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0048] Adjust the temperature of the sea cucumber slurry to 52°C, ad...

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Abstract

The invention relates to a method for preparing composite blood pressure lowering peptide by using a marine organism. The method comprises the following steps of: weighing the following raw materials in percentage by weight: 5-10 percent of sea cucumber, 50-65 percent of jellyfish, 10-25 percent of kelp and 10-20 percent of acetes chinensis; desalting and softening respectively; pulping respectively and then adding water to grind into paste; heating to kill off microorganisms; cooling, then performing enzymatic treatment and deactivating enzyme; cooling and then centrifuging, and filtering supernatant through a tubular membrane to remove macromolecules; and grading filtrate through an organic membrane with molecular weight cutoff of 3,000 Dalton, collecting cutoff liquid of below 3,000 Dalton, and nanofiltering, concentrating, desalting, drying and compounding to obtain the composite blood pressure lowering peptide. In the method, an optimal enzymolysis technical scheme is determined; and in tubular filtration, ultrafiltration, nanofiltration separation extraction technologies, the extraction efficiency is high, and the resultant composite blood pressure lowering peptide has an obvious pressure lowering effect. The method has the advantages of scientific material selection, rational process, easiness and convenience in operation and easiness in implementation of industrial production.

Description

technical field [0001] The invention relates to the preparation of a peptide, in particular to a method for preparing a compound blood pressure-lowering peptide by using marine organisms. Background technique [0002] Antihypertensive peptides, also known as angiotensin converting enzyme (Angiotensin Converting Enzyme, EC3.4.15.1, ACE) inhibitory peptides, are a class of peptides isolated from food proteins that can significantly lower blood pressure, usually small molecular peptide. Among the many antihypertensive substances, antihypertensive peptides have become a hotspot in the research of active peptides because of their non-toxic and side effect antihypertensive function. The antihypertensive peptide isolated from natural food protein, although the antihypertensive effect is not as strong as that of synthetic drugs, but it has high safety, and it only has antihypertensive effect on hypertensive patients, and has no antihypertensive effect on normal blood pressure. Exc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 张绵松胡炜王志伟袁文鹏刘新刘昌衡孙永军唐聚德
Owner 山东好当家海森药业有限公司
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