The invention provides a bacterial strain reproduction method for accelerating restoration of eutrophic water. Acinetobacter, nitrogen and phosphorus removal bacteria, achromobacter xylosoxidans, saccharomyces cerevisiae strains, acinetobacter johnson and pseudomonas can exert different functions in the water and achieve the synergistic effect and mutual promotion, total nitrogen and total phosphorus in the water are effectively removed, the eutrophic water is purified, and growth of pathogenic bacteria can also be inhibited; a culture medium can activate the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas in the dormant state and promote reproduction; an enzyme is beneficial for the reproduction of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacterjohnson and the pseudomonas, and prolongs the proliferative period of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas, and the speed of purifying the eutrophic water is increased. According to the method, the proliferative period of the bacterial strains for eliminating eutrophication can be prolonged, and the reproduction is accelerated, which is beneficial for increasing the speed of restoration for the eutrophic water.