Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate

A Pichia pastoris and hemicellulose technology, applied in the biological field, can solve the problem that it is difficult to achieve high efficiency in biological detoxification

Active Publication Date: 2012-08-15
谷创芯生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of evaporative concentration, or dilute acid steam explosion hydrolysis with low liquid / solid ratio, the concentration of toxic substances in the hydrolyzate will increase with the increase of sugar concentration. Biological detoxification will be difficult to achieve high efficiency

Method used

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  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate
  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate
  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Dry 3 kg of bagasse, add H2SO4 solution (2.5%, w / w) according to the solid-liquid ratio (w / v) of 1:7, hydrolyze for 2 hours at 120 ° C, collect the filtrate by centrifugation, wash the filter residue with water once, combine the filtrates, and adjust them with lye When the pH value reaches 3-7, vacuum concentration is 3-5 times, and the precipitate is removed by suction filtration to obtain 3.5 L of bagasse hemicellulose hydrolyzate. The xylose content of this hydrolyzate is about 12% (w / w).

Embodiment 2

[0072] 5 kg of dry corncobs were crushed to a particle size of less than 1 cm, added 1% (w / w) H2SO4 solution at a solid-to-liquid ratio of 1:10 and soaked for 1 hour, and centrifuged to remove free acid. The pickled corn cobs are put into a 50L steam explosion tank, steam is introduced and the pressure rises to 0.7Mpa for 5 minutes, and then the steam valve is opened to release the pressure and explode instantly. Collect the steam explosion material in the material cavity and collect the steam explosion liquid by centrifugation, neutralize with calcium carbonate to pH 4-7, remove the sediment by suction filtration, and obtain 12L of corncob steam explosion hydrolyzate. The xylose content of this steam explosion hydrolyzate is about 11% (w / w).

Embodiment 3

[0074] According to the ratio of 100g of bagasse to 0.8g of hydrochloric acid, evenly spray 10% hydrochloric acid solution on the raw material of bagasse, and leave it overnight to make the acid liquid diffuse evenly in the bagasse tissue. Put the bagasse that has been acid-treated overnight into a steam explosion tank, feed steam, and maintain it for 3 minutes under the pressure cooking condition of 0.7MPa, then start the explosion and release the pressure instantly. Collect all samples in the material chamber, and collect the liquid part by centrifugal filtration. Wash the residue once with a little water, combine the two filtrates, concentrate twice in vacuum, adjust the pH value to 4-7 with alkali, and remove the precipitate by centrifugation to obtain the bagasse hemicellulose steam-exploded hydrolyzate with a xylose content of about 120g / L.

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Abstract

The invention discloses a novel pichia strain and a method for mixing and culturing the same to biologically detoxify hemicellulose hydrolysate. The novel pichia strain comprises pichia galeiformis CCTCCNO:M 209245 (B-10), pichia occidentalis CCTCC NO:M 209273 (C-4), and pichia kudriavzevii CCTCC NO:M 209274 (D-6) which have degrading activity for 12 known microbial metabolism inhibitors existed in the hemicellulose hydrolysate, has the characteristics of good poison resistance and quick detoxifying process and does not use pentose or pentose alcohol. In the invention, three strains are used for jointly carrying out biological detoxifying fermentation for the hemicellulose hydrolysate, and the xylitol fermentation performance of the treated hydrolysate can reach a fermentation performanceachieved by using pure xylose as stroma. The invention has the advantages of environmental protection and cost saving.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to Pichia pastoris strains isolated from nature, enlarged and mixed for fermenting dilute acid hydrolyzate of hemicellulose, degrading substances toxic to xylitol-producing yeast, and The detoxified hydrolyzate is used in the method of fermenting and producing xylitol. Background technique [0002] Lignocellulose is the main component of the plant cell wall and consists of three major components: cellulose, hemicellulose and lignin. Crop straw is the easiest resource for human society to concentrate on obtaining lignocellulose resources. The cellulose and hemicellulose in it are hydrolyzed into corresponding sugars, and then fermented to produce chemicals needed by human society. This is the transformation of this low-value resource into high-quality An effective way to add value products. [0003] Cellulose is a polymer of glucose, and the hydrolysis product is mainly gluc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P39/00C12P19/02C12R1/84C12R1/74
Inventor 覃香香林兰英张厚瑞卫力
Owner 谷创芯生物科技(厦门)有限公司
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