Method for inducing test-tube bulblet of lily

A technology of small bulbs and lilies, which is applied in the field of induction of lily small bulbs, can solve the problems of species degradation, virus accumulation, small reproduction coefficient, etc., and achieve the effects of preventing species degradation, high survival, and reducing costs

Active Publication Date: 2012-10-31
CHINA THREE GORGES CORPORATION +1
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  • Summary
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  • Description
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AI Technical Summary

Problems solved by technology

[0002] For a long time, lilies have been propagated by the traditional ways of dividing bulbs, bulbils, scale cuttings, scale embedding, etc., which have shortcomings such as small reproduction coefficient, species degradation, and virus accumulation.

Method used

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Examples

Experimental program
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Embodiment Construction

[0019] Differentiation medium preparation: take Ms as the basic medium, add 30g / L sucrose and 5g / L agar powder to the medium, and the hormone combination in the medium is: 6-BA1.5mg / L+NAA0.5mg / L +IBA0.1mg / L;

[0020] Preparation of bulb induction medium: Ms was used as the basic medium, sucrose 30g / L and agar powder 5g / L were added to the medium, and the combination of hormones added in the medium was: 6-BA0.5 mg / L +NAA0.1 mg / L.

[0021] Wherein: 6-BA is 6-benzyladenine, NAA is naphthalic acid, and IBA is indolebutyric acid.

[0022] Induction process example:

[0023] 1) Low-temperature treatment of scales: select healthy lily bulbs, wash them and place them in a refrigerator at 5°C for about 7-10 days;

[0024] 2) Sterilization of explants: Peel off the outer 1-2 layers of scales from the bulbs stored at low temperature, select the inner scales as explants, soak the explants in 5% washing powder solution for 10 minutes and wash them, then place Rinse in running water for...

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Abstract

The invention discloses a method for inducing a test-tube bulblet of lily. The method disclosed by the invention comprises the following steps of: inoculating the lily bulb as an explant into a differential culture medium; then firstly inducing to generate cluster seedlings, and then carrying out low temperature treatment on the cluster seedlings; inoculating the cluster seedlings into a bulb induction culture medium; inducing the test-tube bulblet; carrying out low temperature treatment at the temperature of 10 DEG C for 14 days; and culturing in a constant temperature culture room, thus being beneficial to expansion of the test-tube bulblet. A robust lily bulblet is induced in a test tube, thus growth time of tissue culture seedling can be beneficially shortened and production cost can be reduced.

Description

technical field [0001] The invention relates to a rapid breeding method for lilies, in particular to a method for inducing lily bulblets. Background technique [0002] For a long time, lily has been propagated by the traditional ways of dividing bulbs, bulbils, scale cuttings, scale embedding, etc., but there are shortcomings such as small reproduction coefficient, species degradation, and virus accumulation. Contents of the invention [0003] Aiming at the deficiencies in the prior art, the object of the present invention is to provide a method for inducing lily bulblets that shortens the production time of tissue cultured seedlings, reduces costs, effectively removes viruses in plants, prevents species degradation, and has low cultivation costs. [0004] The object of the present invention is achieved in that a kind of induction method of lily bulblet comprises the following steps: [0005] 1) Low-temperature treatment of scales: select robust lily bulbs, wash and refri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邱利文张国禹黄桂云马晓波胡梅香吴笛张海波
Owner CHINA THREE GORGES CORPORATION
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