Method for preparing thallus protein extractum from bacterial residue of amino acid fermentation liquor

A technology of bacterial protein and fermentation liquid, which is applied in the direction of fermentation and microbial dissolution, which can solve the problems of no obvious increase in yield and less products, and achieve the effects of increasing the smoothness of separation, improving the color of the solution, and improving the filtration efficiency

Pending Publication Date: 2019-11-08
宜昌三峡普诺丁生物制药有限公司
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  • Claims
  • Application Information

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Problems solved by technology

The inventor tries multiple enzymes to carry out enzymolysis, but obtains seldom product all the time, and yield is only below 10%, finds that bacterium is almost without damage under the microscopic examination of enzymolysis liquid ( figure 1 ); then use lysozyme to treat the thalline before enzymolysis, and then enzymolysis, the yield is still not significantly improved

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  • Method for preparing thallus protein extractum from bacterial residue of amino acid fermentation liquor
  • Method for preparing thallus protein extractum from bacterial residue of amino acid fermentation liquor
  • Method for preparing thallus protein extractum from bacterial residue of amino acid fermentation liquor

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Embodiment 1

[0035] A method for preparing bacterial protein extract from bacterial residue of amino acid fermentation liquid, the steps of which are:

[0036] S1: Filtrate the amino acid fermentation liquid with a ceramic membrane, get the thick slurry bacterial residue solution, and measure the protein content by the Kjeldahl method;

[0037] S2: after the high temperature and high pressure treatment is carried out with the concentrated pulp bacterial residue solution liquid, add protease to carry out enzymolysis;

[0038] S3: After the enzymolysis reaction is completed, add a mixed solution of activated carbon and diatomaceous earth under stirring, stir evenly and let stand for about 30 minutes, and filter to obtain a decolorizing solution;

[0039] S4: Extraction preparation: the decolorization solution is concentrated under reduced pressure to obtain bacterial protein extract.

[0040] Preferably, the protein content of step S1 is 10%.

[0041] Preferably, in the step S2, the high t...

Embodiment 2

[0066] On the basis of Example 1, activated carbon, diatomaceous earth, and chitosan in the decolorization process of the step S3 are pretreated and then added to the filtration operation process. The specific pretreatment method is activated carbon, diatomite, carboxylate Methyl chitosan is added to the aqueous solution at a mass ratio of 1:4:0.1 to obtain a mixed solution with a mass concentration of carboxymethyl chitosan of 3g / L. It is ready-to-use and is added to the reaction solution in a corresponding proportion when used. .

[0067] Table 9

[0068] Chitosan Chitosan free Bacterial residue thick slurry volume (ml) 500 500 Activated carbon dosage (W / V) 1％ 1％ Chitosan dosage (W / V) 1‰ - Diatomaceous earth dosage (W / V) 4％ 4％ Filtration vacuum pressure (Mpa) -0.1 -0.1 Filtration time 0.5h 1.2h Filtrate transmittance 86％ 75％

[0069] It can be seen from Table 9 that after adding carboxymethyl chitosan, carbo...

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Abstract

The invention relates to a method for preparing thallus protein extractum from bacterial residue of amino acid fermentation liquor. The method comprises the following steps of: filtering amino acid fermentation liquor with a ceramic membrane, taking a concentrated slurry fungus residue solution, and measuring the protein content by a Kjeldahl determination method; adjusting the pH value of the concentrated slurry fungus residue solution to 7.0-8.5 by using alkali, carrying out high-temperature high-pressure treatment, and adding protease for enzymolysis; after the enzymolysis reaction is finished, preserving heat, adding activated carbon, and performing decolorizing and filtering to obtain a decolorized liquid; and preparing the extractum: concentrating the decolorized liquid under reducedpressure to obtain the thallus protein extractum. The method subjects amino acid fermentation thalli to high-temperature high-pressure wall breaking, enzymolysis, filtration and concentration so as to obtain the extractum with a micromolecule bioactive peptide (namely, oligopeptide) content exceeding 90%, thereby greatly improving the added value, wherein the market price of the thallus crude protein is about 2500 thousand yuan per ton, and the market price of the extractum is about 20-30 thousand yuan per ton.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, in particular to a method for preparing bacterial protein extract from bacterial residue of amino acid fermentation liquid. Background technique [0002] The production of amino acids by microbial fermentation will produce a large amount of bacterial residues, which are rich in organic substances such as bacterial proteins. If they are directly treated as wastewater, a large amount of cost will be invested, which will increase the burden of environmental protection treatment; some enterprises will use them to remove water and dry them It can be made into feed rich in crude protein, or used in the production of compound fertilizers. Although it can relatively reduce investment and even obtain certain economic benefits, the added value is low, generally around 2000 yuan / kg; Alkaline hydrolysis to obtain a mixed solution of various amino acids, which is then used to produce feed rich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12P21/06
CPCC12N1/06C12P21/06
Inventor 张国龙曾宪亮郑炜
Owner 宜昌三峡普诺丁生物制药有限公司
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