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Bacterial strain reproduction method for accelerating restoration of eutrophic water

A technology of eutrophication and water body, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of slow growth rate, short colony growth period, complicated equipment, etc., and achieve faster reproduction. , the effect of prolonging the proliferative period

Inactive Publication Date: 2019-08-09
黄山市益天士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the commonly used physical and chemical restoration methods are generally expensive in investment, complicated in equipment, and not suitable for the restoration and treatment of large-scale water pollution.
However, microbial restoration technology cannot be widely used due to limitations such as short colony proliferation period and slow growth rate.

Method used

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  • Bacterial strain reproduction method for accelerating restoration of eutrophic water
  • Bacterial strain reproduction method for accelerating restoration of eutrophic water
  • Bacterial strain reproduction method for accelerating restoration of eutrophic water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The method for accelerating the proliferation of bacteria in repairing eutrophic water body comprises the following steps:

[0036] a) Soak apple powder, tomato powder, marigold powder and carrot powder with a particle size of 10-20 mesh in water for 1 hour, heat to 60°C for 3 hours, pass through a 300-500-mesh sieve after cooling, and filter the obtained filtrate After concentration, mix with glucose, agar, and beef broth, and adjust the pH to 6 with a pH regulator to obtain a culture medium;

[0037] The mass ratio of apple powder, tomato powder, calendula powder, carrot powder, water, filtrate, glucose, agar, and beef broth is 5:5:1:12:100:5:1:0.5:8:1:10; The volume ratio of the concentrated filtrate to water is 1:10.

[0038] b) After the medium is sterilized under high temperature and high pressure, take 6 parts and cultivate Acinetobacter, denitrification and phosphorus removal bacteria, Achromobacter xylosoxidans, Saccharomyces cerevisiae strains, Acinetobacter ...

Embodiment 2

[0049] The method for accelerating the proliferation of bacteria in repairing eutrophic water body comprises the following steps:

[0050] a) Soak apple powder, tomato powder, marigold powder and carrot powder with a particle size of 10-20 mesh in water for 1 hour, heat to 80°C for 2 hours, pass through a 300-500-mesh sieve after cooling, and filter the obtained filtrate After concentration, mix with glucose, agar, and beef broth, and adjust the pH to 8 with a pH regulator to obtain a culture medium;

[0051] The mass ratio of apple powder, tomato powder, calendula powder, carrot powder, water, filtrate, glucose, agar, beef broth is 10:3:2:10:150:3:2:0.4:10:1:15; The volume ratio of concentrated filtrate to water is 1:15.

[0052] b) After the culture medium was sterilized under high temperature and high pressure, 6 parts were taken and cultured respectively at room temperature for Acinetobacter, Nitrogen and Phosphorus Removal bacteria, Achromobacter xylosoxidans, Saccharomy...

Embodiment 3

[0063] The method for accelerating the proliferation of bacteria in repairing eutrophic water body comprises the following steps:

[0064] a) Soak apple powder, tomato powder, marigold powder and carrot powder with a particle size of 10-20 mesh in water for 1 hour, heat to 70°C for 2 hours, pass through a 300-500-mesh sieve after cooling, and filter the obtained filtrate After concentration, mix with glucose, agar, and beef broth, and adjust the pH to 7 with a pH regulator to obtain a culture medium;

[0065] The mass ratio of apple powder, tomato powder, calendula powder, carrot powder, water, filtrate, glucose, agar, and beef broth is 8:4:1.5:11:120:4:1.5:0.45:9:1:12; The volume ratio of concentrated filtrate to water is 1:13.

[0066] b) After the medium is sterilized under high temperature and high pressure, take 6 parts and cultivate Acinetobacter, denitrification and phosphorus removal bacteria, Achromobacter xylosoxidans, Saccharomyces cerevisiae strains, Acinetobacter...

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Abstract

The invention provides a bacterial strain reproduction method for accelerating restoration of eutrophic water. Acinetobacter, nitrogen and phosphorus removal bacteria, achromobacter xylosoxidans, saccharomyces cerevisiae strains, acinetobacter johnson and pseudomonas can exert different functions in the water and achieve the synergistic effect and mutual promotion, total nitrogen and total phosphorus in the water are effectively removed, the eutrophic water is purified, and growth of pathogenic bacteria can also be inhibited; a culture medium can activate the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas in the dormant state and promote reproduction; an enzyme is beneficial for the reproduction of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacterjohnson and the pseudomonas, and prolongs the proliferative period of the acinetobacter, the nitrogen and phosphorus removal bacteria, the achromobacter, the xylosoxidans, the saccharomyces cerevisiae strains, the acinetobacter johnson and the pseudomonas, and the speed of purifying the eutrophic water is increased. According to the method, the proliferative period of the bacterial strains for eliminating eutrophication can be prolonged, and the reproduction is accelerated, which is beneficial for increasing the speed of restoration for the eutrophic water.

Description

technical field [0001] The invention relates to the field of strain propagation, in particular to a strain propagation method for accelerating restoration of eutrophic water bodies. Background technique [0002] Eutrophication is a global environmental problem. Although the phenomenon of eutrophication has been widely known, its mechanism is still poorly understood, which adds a lot of difficulty to the prevention and control of eutrophication. Among the various elements that affect productivity, nitrogen and phosphorus are the most common limiting nutrient factors, so controlling nitrogen and phosphorus loads has become the focus of eutrophication control in many aquatic ecosystems. At present, the most commonly used measure to control eutrophication is to control the inflow of exogenous pollutants. Although the control of point source pollution is relatively easy to operate, it is difficult to fully implement the control of non-point source pollution. In addition, sediment...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N1/20C02F3/34C12R1/865C12R1/01C12R1/38
CPCC02F3/34C02F3/347C12N1/18C12N1/20
Inventor 汪军陈庆裕
Owner 黄山市益天士生物科技有限公司
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