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Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate

A Pichia pastoris and hemicellulose technology, applied in the biological field, can solve the problem that it is difficult to achieve high efficiency in biological detoxification

Active Publication Date: 2011-04-13
谷创芯生物科技(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of evaporative concentration, or dilute acid steam explosion hydrolysis with low liquid / solid ratio, the concentration of toxic substances in the hydrolyzate will increase with the increase of sugar concentration. Biological detoxification will be difficult to achieve high efficiency

Method used

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  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate
  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate
  • Novel pichia strain and method for mixing and culturing same to biologically detoxify hemicellulose hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Dry bagasse 3kg, add H2SO4 solution (2.5%, w / w) according to the solid-liquid ratio (w / v) of 1:7, hydrolyze at 120℃ for 2h, collect the filtrate by centrifugation, wash the filtrate once with water, combine the filtrate and adjust with lye The pH value is 3-7, vacuum concentration is 3 to 5 times, and the precipitate is removed by suction filtration to obtain 3.5L of bagasse hemicellulose hydrolysate. The xylose content of this hydrolyzate is about 12% (w / w).

Embodiment 2

[0072] Dry corn cob 5kg, crushed to a particle size of 1cm or less, add 1% (w / w) H2SO4 solution to soak for 1 hour at a solid-liquid ratio of 1:10, and centrifuge to remove free acid. The soaked corncobs are put into a 50L steam explosion tank, steam is injected and the pressure is increased to 0.7Mpa for 5 minutes, and then the steam valve is opened to release the pressure and burst instantly. Collect the steam explosion material in the material cavity and collect the steam explosion liquid by centrifugal separation, neutralize the calcium carbonate to a pH value of 4-7, and remove the sediment by suction filtration to obtain 12L of corncob steam explosion hydrolysate. The xylose content of this steam explosion hydrolysate is about 11% (w / w).

Embodiment 3

[0074] According to the ratio of 100 g bagasse to 0.8 g hydrochloric acid, the 10% hydrochloric acid solution was evenly sprayed on the bagasse raw material, and the solution was left overnight to make the acid solution diffuse evenly in the bagasse tissue. Put the bagasse that has been acid-treated overnight in a steam explosion tank, put in steam, maintain it for 3 minutes under a pressure of 0.7MPa, and then detonate to release the pressure instantly. Collect all the samples in the material cavity, and collect the liquid part by centrifugal filtration. The residue is washed once with a little water, the two filtrates are combined, and concentrated twice in vacuum. The pH value is adjusted to 4-7 with alkali, and the precipitate is removed by centrifugation to obtain the bagasse hemicellulose steam explosion hydrolysate, the xylose content of which is about 120g / L.

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Abstract

The invention discloses a novel pichia strain and a method for mixing and culturing the same to biologically detoxify hemicellulose hydrolysate. The novel pichia strain comprises pichia galeiformis CCTCCNO:M 209245 (B-10), pichia occidentalis CCTCC NO:M 209273 (C-4), and pichia kudriavzevii CCTCC NO:M 209274 (D-6) which have degrading activity for 12 known microbial metabolism inhibitors existed in the hemicellulose hydrolysate, has the characteristics of good poison resistance and quick detoxifying process and does not use pentose or pentose alcohol. In the invention, three strains are used for jointly carrying out biological detoxifying fermentation for the hemicellulose hydrolysate, and the xylitol fermentation performance of the treated hydrolysate can reach a fermentation performance achieved by using pure xylose as stroma. The invention has the advantages of environmental protection and cost saving.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and particularly relates to Pichia pastoris strains isolated from the natural world, expanded and mixed to ferment the dilute acid hydrolysate of hemicellulose, to degrade substances toxic to xylitol-producing yeast, and This detoxified hydrolysate is used in the method of fermentative production of xylitol. Background technique [0002] Lignocellulose, the main component of plant cell walls, is composed of cellulose, hemicellulose and lignin. Crop straw is the easiest resource for human society to obtain lignocellulose. The cellulose and hemicellulose are hydrolyzed into the corresponding sugars, and then the chemical products needed by human society are fermented to produce high-value resources. An effective way to add value products. [0003] Cellulose is a polymer of glucose, and the hydrolyzate is mainly glucose. Cellulose in its natural state tends to form a compact structure similar to cry...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P39/00C12P19/02C12R1/84C12R1/74
Inventor 覃香香林兰英张厚瑞卫力
Owner 谷创芯生物科技(厦门)有限公司
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