Biological tissue material and preparation method thereof

Abstract

The invention discloses a dry-state biological tissue material and a preparation method thereof.The preparation method comprises the steps that decellularization treatment is conducted on the biological tissue material through decellularization solutions, and the decellularization solutions comprise the first decellularization solution to the Nth decellularization solution with the concentration gradually increased; carrying out first-time anti-calcification treatment by adopting a dissolving solution, wherein the dissolving solution is used for dissolving phospholipid impurities in the biological tissue; carrying out second-time anti-calcification treatment by adopting a removal solution, wherein the removal solution is used for removing an oxidation product carboxyl of an aldehyde group in the cross-linking and decellularization processes; carrying out third-time anti-calcification treatment by adopting a reduction solution, wherein the reduction solution is used for protecting free aldehyde groups; dehydration solutions are adopted for dehydration treatment, the dehydration solutions comprise the first dehydration solution to the Mth dehydration solution with the concentration gradually increased, the concentration of the Mth dehydration solution is smaller than that of the Nth decellularization solution, the purposes of dissolving phospholipid, removing carboxylic acid and protecting aldehyde groups are achieved through three times of anti-calcification treatment, and a complete anti-calcification process is achieved; and high calcification resistance is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biological tissue material and a preparation method thereof. Background technique [0002] Currently, commonly used clinical biological tissues (such as animal-derived collagen fibrous tissue materials used in biological valves, biological patches, etc.) are usually cross-linked and fixed using chemical reagents (such as glutaraldehyde and / or formaldehyde storage). Biological tissue is generally preserved in a dilute aqueous solution containing glutaraldehyde and / or formaldehyde so that the components of the biological tissue can be kept sterile and hydrated. However, a large number of studies have proved that after the implantation of biological tissue prostheses, the residual glutaraldehyde will promote the calcification of the valve, and glutaraldehyde has high toxicity, and even a very low residual amount will cause toxicity in the human body. Conducive to the formation of end...

AI Technical Summary

Problems solved by technology

However, a large number of studies have shown that there are many problems in the above methods, such as insufficient decellularization leads to immune response or excessive decellularization leads to collagen structure damage; after biomaterials are implanted in vivo, the adsorption of lipoproteins and calcium-binding proteins by phospholipids on the surface of biological tissues May cause calcification, which can lead to hardening or degradation of biological material

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