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Biological material, preparation method and application thereof

A biomaterial and cross-linking reaction technology, applied in the field of biomedical materials, can solve the problems of bio-heart valves such as easy degradation, achieve endothelialized barrier, improve structural stability and mechanical strength, and prolong the use time

Active Publication Date: 2022-02-11
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, glutaraldehyde-crosslinked biological heart valves are prone to degradation and calcification, resulting in only about 10 years of effective service life for biological valves.

Method used

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  • Biological material, preparation method and application thereof
  • Biological material, preparation method and application thereof
  • Biological material, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039] The preparation method of the amnion basement membrane double-layer cross-linking unit is as follows: soak the amnion basement membrane in an aqueous solution containing small molecular substances of amino groups; then place the soaked amnion basement membrane The crosslinking reaction occurs in the aqueous solution of imide.

[0040]It should be noted that the soaked decellularized amniotic membrane or amnion basement membrane is placed in an aqueous solution containing carbodiimide and N-hydroxysuccinimide, and carbodiimide activates the carboxyl groups of the decellularized amnion or amnion basement membrane , and N-hydroxysuccinimide makes the activated carboxyl group and amino group undergo stable cross-linking, and dehydration condensation forms peptide bonds, thereby realizing the stable cross-linking of other extracellular matrices except collagen, including elastin. couplet.

[0041] Specifically, the decellularized amnion or amniotic basement membrane is plac...

Embodiment 1

[0067] The embodiment of the present application provides a method for preparing a biological material, comprising the following steps:

[0068] S1. Acquisition of amnion: Obtain the amnion in a sterile environment, soak it in saline containing penicillin, streptomycin and penicillin for 30 minutes, wash it several times to remove the blood, and then bluntly separate the amnion from the chorion of the placenta , spread its upper surface on a sterilized cellulose acetate membrane, and set aside;

[0069] S2. Acquisition of amniotic basement membrane: the amniotic membrane is separated from the fibroblast layer, spongy layer, epithelial cell layer and compact layer, then dehydrated and decellularized, and washed with saline containing penicillin and streptomycin to obtain the amniotic basement membrane ;

[0070] S3. Acquisition of amniotic basement membrane fluid: after the amniotic basement membrane is crushed, trypsin and nuclease are added for enzymatic hydrolysis, and then...

Embodiment 2

[0077] The embodiment of the present application provides a method for preparing a biological material, comprising the following steps:

[0078] S1. Acquisition of amnion: Obtain the amnion in a sterile environment, soak it in saline containing penicillin, streptomycin and penicillin for 30 minutes, wash it several times to remove the blood, and then bluntly separate the amnion from the chorion of the placenta , spread its upper surface on a sterilized cellulose acetate membrane, and set aside;

[0079] S2. Acquisition of amniotic basement membrane: the amniotic membrane is separated from the fibroblast layer, spongy layer, epithelial cell layer and compact layer, then dehydrated and decellularized, and washed with saline containing penicillin and streptomycin to obtain the amniotic basement membrane ;

[0080] S3. Soak the amniotic basement membrane in an aqueous solution of lysine with a mass concentration of 4% for 24 hours, then place the amniotic basement membrane in wat...

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Abstract

The invention provides a biological material, a preparation method and application thereof. The biological material comprises a biological material body, water-water micro-droplets and an amniotic basement membrane liquid surface layer, or the biological material comprises two amniotic basement membrane double-layer cross-linking units and water-water micro-droplets located between the amniotic basement membrane double-layer cross-linking units. According to the invention, water-water micro-droplets are used as a biological cross-linking agent to cross-link an amniotic basement membrane liquid surface layer and a biological material body, or cross-link an amniotic basement membrane double-layer cross-linking unit to retain the non-immunogenicity of the amniotic membrane and the amniotic basement membrane, and the surface layer formed by amniotic basement membrane liquid and the amniotic basement membrane double-layer cross-linking unit can realize rapid endothelialization and block calcium salt deposition, so that the anti-calcification performance of the biological material is improved, and the service time of the biological material is prolonged; and the structural stability and the mechanical strength of the whole biological material are improved by adopting a double-crosslinking mode.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a biomaterial and its preparation method and application. Background technique [0002] Heart valve disease is a common disease of valve degeneration. Anatomically, it manifests as narrowing of the blood passage or valvular insufficiency. Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. Mechanical valves are durable, but require life-long anticoagulant drugs, which have a high incidence of thromboembolism and may cause bleeding complications. Biological valves have similar hydrodynamic propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/14A61L27/26A61L27/50A61L27/60
CPCA61L27/3604A61L27/3687A61L27/14A61L27/60A61L27/26A61L27/50A61L2430/20A61L2430/40A61L2430/02C08L89/00C08L5/02C08L71/02
Inventor 周辉王月虹
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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