Biological material based on amniotic basement membrane, preparation method and application thereof

A biomaterial and basement membrane technology, applied in the field of biomedical materials, can solve the problems of not being able to directly apply biological valves, promoting the formation of calcium phosphate crystals, and calcification and decay of biological valves, so as to improve the anti-calcification performance, prolong the use time, and block calcium. Effects of salt deposition

Pending Publication Date: 2022-02-11
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, amniotic membrane has low toughness and poor durability, so it cannot be directly applied to biological valves
The use of chemical cross-linking agents such as glutaraldehyde and genipin can cross-link the single-layer amniotic membrane into multi-layer amniotic membrane, which enhances the toughness and durability of use, b

Method used

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  • Biological material based on amniotic basement membrane, preparation method and application thereof
  • Biological material based on amniotic basement membrane, preparation method and application thereof
  • Biological material based on amniotic basement membrane, preparation method and application thereof

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preparation example Construction

[0028] The embodiment of the present application provides a biomaterial based on amnion basement membrane, such as figure 1 As shown, it includes: biological material body 1, water micro-droplet 3 and outer layer of amnion basement membrane 2, water micro-droplet 3 is located between biomaterial body 1 and outer layer of amnion basement membrane 2, water micro-droplet 3. Connect the outer layer 2 of the amnion basement membrane to the outer surface of the biomaterial body 1; the preparation method of the biomaterial body 1 includes the following steps: reacting the polymer biomaterial, the cross-linking agent and the initiator at 65-75°C for 20-24 hours , and then heated up to 95-100° C. to continue the reaction for 20-24 hours to obtain the biomaterial body.

[0029] It should be noted that, in the amnion basement membrane-based biological material of the embodiment of the present application, the water micro-droplets are used as a biological cross-linking agent to cross-link...

Embodiment 1

[0055] The embodiment of the present application provides a method for preparing a biomaterial based on amniotic membrane basement membrane, comprising the following steps:

[0056] S1. Acquisition of amnion: Obtain the amnion in a sterile environment, soak it in saline containing penicillin, streptomycin and penicillin for 30 minutes, wash it several times to remove the blood, and then bluntly separate the amnion from the chorion of the placenta , spread its upper surface on a sterilized cellulose acetate membrane, and set aside;

[0057] S2. Acquisition of amniotic basement membrane: the amniotic membrane is separated from the fibroblast layer, spongy layer, epithelial cell layer and compact layer, then dehydrated and decellularized, and washed with saline containing penicillin and streptomycin to obtain the amniotic basement membrane ;

[0058] S3. Soak the amniotic basement membrane in an aqueous solution of lysine with a mass concentration of 4% for 24 hours, then place ...

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Abstract

The invention provides a biological material based on an amniotic basement membrane, a preparation method and application thereof. The biological material comprises a biological material body, water micro-droplets and an amnion basement membrane outer surface layer. According to the biological material disclosed by the invention, water-water micro-droplets are used as a biological cross-linking agent to cross-link the amnion basement membrane outer surface layer and the biological material body to reserve the non-immunogenicity of the amnion basement membrane, the outer surface layer of the amnion basement membrane can realize rapid endothelialization and obstruct calcium salt deposition, the anti-calcification performance of the biological material is improved, and the service life of the biological material based on the amnion basement membrane is prolonged; stable cross-linking of most collagen tissues can be realized by the water-water micro-droplets, the structural stability and mechanical strength of the whole biological material are improved, and the use of a chemical cross-linking agent is avoided, so that the protein activity of the biological material is prevented from being damaged; and according to the whole biological material, more crosslinking sites are provided through a double-crosslinking method of water-water micro-droplets and a chemical condensation effect, so that the structural stability and the mechanical strength of the biological material are improved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a biomaterial based on amniotic membrane basement membrane and its preparation method and application. Background technique [0002] Heart valve disease, vascular valve disease, periosteum disease, and blood-brain barrier disease need to be treated by replacing biological valves. Surgery is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which many patients cannot accept. Mechanical valves are durable, but require life-long anticoagulant drugs, which have a high incidence of thromboembolism and may cause bleeding complications. Biological valves have similar hydrodynamic properties to human heart valves and are as biocompatible as mechanical valves, and patients only need short-term or no anticoagulant drugs. However, biological valves are prone to calcification, have poor durability, and have certain immunogenicity. [0003] T...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36A61L27/14A61L27/26A61L27/50A61L27/60
CPCA61L27/3604A61L27/3687A61L27/14A61L27/60A61L27/26A61L27/50A61L2430/20A61L2430/40A61L2430/02C08L89/00C08L5/02C08L71/02
Inventor 周辉王月虹
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
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