Biological material based on amniotic basement membrane, preparation method and application thereof

Abstract

The invention provides a biological material based on an amniotic basement membrane, a preparation method and application thereof. The biological material comprises a biological material body, water micro-droplets and an amnion basement membrane outer surface layer. According to the biological material disclosed by the invention, water-water micro-droplets are used as a biological cross-linking agent to cross-link the amnion basement membrane outer surface layer and the biological material body to reserve the non-immunogenicity of the amnion basement membrane, the outer surface layer of the amnion basement membrane can realize rapid endothelialization and obstruct calcium salt deposition, the anti-calcification performance of the biological material is improved, and the service life of the biological material based on the amnion basement membrane is prolonged; stable cross-linking of most collagen tissues can be realized by the water-water micro-droplets, the structural stability and mechanical strength of the whole biological material are improved, and the use of a chemical cross-linking agent is avoided, so that the protein activity of the biological material is prevented from being damaged; and according to the whole biological material, more crosslinking sites are provided through a double-crosslinking method of water-water micro-droplets and a chemical condensation effect, so that the structural stability and the mechanical strength of the biological material are improved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a biomaterial based on amniotic membrane basement membrane and its preparation method and application. Background technique [0002] Heart valve disease, vascular valve disease, periosteum disease, and blood-brain barrier disease need to be treated by replacing biological valves. Surgery is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which many patients cannot accept. Mechanical valves are durable, but require life-long anticoagulant drugs, which have a high incidence of thromboembolism and may cause bleeding complications. Biological valves have similar hydrodynamic properties to human heart valves and are as biocompatible as mechanical valves, and patients only need short-term or no anticoagulant drugs. However, biological valves are prone to calcification, have poor durability, and have certain immunogenicity. [0003] T...

AI Technical Summary

Problems solved by technology

However, amniotic membrane has low toughness and poor durability, so it cannot be directly applied to biological valves

The use of chemical cross-linking agents such as glutaraldehyde and genipin can cross-link the single-layer amniotic membrane into multi-layer amniotic membrane, which enhances the toughness and durability of use, but the phosphate groups and chemical cross-linking agents contained in cell fragments or cell membranes The exposure of carboxyl groups, aldehyde groups, amino groups and other groups of collagen fibers caused by cross-linking will promote the formation of calcium phosphate crystals and cause the calcification of biological valves.

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