69results about How to "Rapid endothelialization" patented technology

The invention relates to medical equipment, particularly a medical scaffold with an inner coating film, and a preparation method for the medical scaffold, and belongs to the field of medical equipment. In the medical scaffold with the inner coating film, a non-woven inner coating film of which fibers are distributed on the axial direction of the medical scaffold is prepared on the inner surface of an inner film by an electrospinning method by taking a coating film scaffold as a basis, wherein the coating film scaffold consists of a netlike scaffold which is formed by weaving medical alloy wires, an electrospinning non-woven inner film and an electrospinning non-woven outer film; and the thickness of the inner coating film is 2 to 6 mu m. During preparation of the medical scaffold with the inner coating film, the inner coating film, the inner film and the outer film are prepared sequentially, and the inner coating film in the medical scaffold improves the biocompatibility with tract tissues of a human body, facilitates adhesion, migration and reproduction of endothelial cells at two ends of the medical scaffold, promotes quick endothelialization of the medical scaffold, and prevents the medical scaffold from becoming narrow again. The preparation method for the medical scaffold is simple, convenient to operate, and low in cost, and suitable for preparation of medical scaffolds with different diameters and lengths.

The invention relates to a composite drug stent and a preparation method thereof. The stent includes a stent body and active drugs; wherein, the stent body is a perforated medical material with good biocompatibility, which can be made by optionally using stainless steel, cobalt-based alloy, titanium alloy, nickel-titanium alloy, or polylactic acid bio-polymer material; the active drugs include special endothelial progenitor cell antibody drug and anti-smooth muscle cell proliferation drug. The internal surface of the perforated stent body is fixedly provided with the special endothelial cell antibody drug, while the external surface of the perforated stent body is coated with the anti-smooth muscle cell proliferation drug. The preparation method includes as follows: (1) pretreating the surface of the stent body; (2) making holes; (3) post-treating the surface of the stent body; (4) dispensing medicines; (5) coating the external surface; (6) fixing the internal surface; (7) drying at low temperature. The preparation method can selectively absorb endothelial progenitor cells and facilitate in repairing endodermis, and can effectively inhibit the proliferation and migration of vascular smooth muscle cells, persistently and effectively reduce the newborn endangium, effectively prevent the stent from narrowing, and avoid the risk of tardive thrombosis.

The invention discloses a valve clamping device. The valve clamping device comprises a first clamping component, a second clamping component and a fixing component. The fixing component is used for fixing the first clamping component and the second clamping component. The first clamping component comprises at least two first clamping arms. The second clamping component comprises a corresponding number of second clamping arms. A pair of clamps can be formed by each first clamping arm and the corresponding second clamping arm. The valve clamping device has the advantages that the valve clampingdevice can employ the smooth-transition top design, the high-clamping-stability clamping tooth design and the 'one-stripe-for-dualpurpose' fixing ring deign, so that damage to valves during movement of the clamping device is reduced, users capture the valves conveniently, and clamping performance is improved while damage to the valves is reduced; the overall structure of the clamping device has high machinability, and production and manufacture of factories are facilitated.

The invention discloses a preparation method of a surface coating of intravascular stents or cardiac valves with excellent biocompatibility, which includes the steps: A. the preparation of a coating solvent: heparin is dissolved in deionized water, the pH value thereof is adjusted to 7 to 8 and then an endothelial cell growth factor is added to form a heparin / growth factor solution; collagens are dissolved in acetum to form a collagen solution; B. dip-coating: the intravascular stents or the cardiac valves are immersed in the heparin / growth factor solution and a layer of heparin / growth factor is dipped and coated on the surface of the intravascular stents or the cardiac valves, and then the intravascular stents or the cardiac valves are taken out for being cleaned by the deionized water and being dried by nitrogen gas; and the intravascular stents or the cardiac valves are immersed in the collagen solution, coated with a layer of collagen, and taken out for being cleaned by the deionized water and being dried by nitrogen gas; the steps of dip-coating by the use of the heparin / growth factor and the collagen are repeated in sequence for 1 time to 60 times; finally the step of dip-coating by the use of the heparin / growth factor is carried out again to obtain the surface coating with excellent biocompatibility of the intravascular stents or the cardiac valves. The intravascular stents or the cardiac valves prepared have excellent biocompatibility and low preparation cost.

The invention discloses a method which adopts the surface of a titanium and titanium alloy cardiovascular implantation device to fix CD34 antibody or CD133 antibody directionally, and comprises the following main steps: A. activation treatment is carried out, so as to form hydroxyl on the surface; B. a layer of avidin is adsorbed on the activation surface of the step A; C. a layer of biotinylated protein A is adsorbed by the avidin layer; D. on the surface of the protein A layer obtained, a layer of specific antibodies of the membrane protein of endothelial progenitor cells- CD34 antibodies or CD133 antibodies are fixed directionally, so the titanium and titanium alloy cardiovascular implantation device can capture the endothelial progenitor cells in blood after being implanted into the human body, thereby leading to rapid endothelialization of the implantation device surface and leading the implantation device to have excellent anticoagulant function and restenosis occurring resistance function.

A vascular stent and a method for manufacturing same. The vascular stent has a mesh-shaped tubular structure. The mesh-shaped tubular structure is formed by braiding a metal wire and a biodegradable wire. The vascular stent has the following advantages: First, the stent has a thinner wall, facilitating implantation of the vascular stent into a narrower blood vessel and reducing the risk of restenosis after the stent is implanted in a small blood vessel. Secondly, the rejection caused by the presence of foreign bodies in the human body is reduced, and endothelialization of tissues is promoted. Thirdly, the vascular stent is allowed to have a greater mesh density, so that hemodynamics at the lesion site in the blood vessel can be improved after the vascular stent is implanted in the human body.

The invention provides an endovascular stent modification method with the combination of a nanometer coating and a microporous surface, which comprises the steps of: forming micropores on the surface of an implantable medical device, namely an NiTi alloy or 316L stainless steel endovascular stent, preparing a layer of SiOx: H nanometer coating on the surfaces of the micropores, ensuring that the stent can be quickly endothelialized after implanted into a human body and has good blood compatibility and corrosion resistance, and selecting a trimethyl silane (TMS) monomer or mixed gas of the trimethyl silane monomer and oxygen O2 as reaction gas aerated for preparing the nanometer coating, or orderly aerating the TMS, CH4 and O2 gas monomers respectively. The stent modified by the method can be quickly endothelialized after implanted into a pathological change position, and effectively reduce the formation of thrombus; and the biocompatibility (such as corrosion resistance, and the like) of the stent is obviously superior to that of an unmodified stent, so that the incidence rate of ISR is reduced after the sent is implanted through surface modification technology based on stents which have come into the market.

Provided are a coating structure of a medical biodegradable zinc alloy stent and a preparation method thereof. The stent consists of a coating covering the surface of a zinc alloy stent body (Zn), andcomprises three layers of S1, S2 and S3, the S1 is an oxide coating, the S2 is a polymer coating, and the S3 is a drug coating.

The present invention relates to a novel biodegradable vascular stent preparation method, which specifically comprises: taking a raw material, heating to achieve a melting state, and cooling to a room temperature to obtain a blank material; adopting a self-made mold, a temperature control device, a heating rod, a clamping device, a data acquisition card, a computer monitoring system and other components to form a set of preparation equipment, paving the blank material in the mold cavity, placing the mold in the clamping device after the blank material achieves a set temperature, tightening the clamping device, and carrying out thermal insulation, cooling, mold opening, polishing and cleaning to obtain a primary stent; and placing the stent in an electrostatic spinning device to carry out electrostatic spinning direct-writing to prepare the composite mesh film stent. The prepared stent has characteristics of degradability, smooth surface and substantially increased mechanical strength, wherein a radial strength of the stent can be effectively enhanced and malformation proliferation hyperplasia of vascular endothelium on the stent can be inhibited with the mesh film. In addition, the whole preparation process is easy and easy to performe, and great practical significances are provided for treatment of cadiovascular or lumen stenosis diseases.

The invention discloses a coating stent and a preparation method thereof, the coating stent is of a net structure, a component forming the net structure is composed of three layers of structures, the inner layer is a stent layer, the middle layer is an anticoagulant modified layer, and the outermost layer is a nano oligopeptide coating; the coating stent not only can resist blood coagulation and play a role in preventing thrombus, but also can promote rapid endothelialization of vascular cells and effectively promote cell regeneration of a vascular lesion area; and the whole production process is simple, pollution-free to the environment and low in cost.

The invention relates to a composite artificial blood vessel and a preparation method thereof. The artificial blood vessel has a double-layer tubular structure, and comprises a first nanofiber layer arranged on the inner layer and a second nanofiber layer arranged on the outer layer, wherein the first nano-fiber layer and the second nano-fiber layer are connected in an adhering manner, the first nanofiber layer is formed by rolling one, two or more layers of nanofiber membranes, the second nanofiber layer has an integrally formed structure, and microporous structures are arranged on the firstnanofiber layer and the second nanofiber layer. According to the invention, the degradable nanofiber small-caliber artificial blood vessel achieves three-dimensional infiltration of autologous cells and regeneration of new tissues before a large number of fibers begin to be degraded, so that the blood vessel still has good strength and good elasticity when the fibers are degraded in a large number.

The invention discloses an acellular dermis matrix enhanced thermally-induced phase separation nanofiber small-bore tubular stent and a preparation method thereof. The preparation method comprises the following steps: performing decellularization treatment on aorta of healthy animals; placing the matrix obtained by decellularization on a watch glass, dropwise adding ultrapure water to submerge all the matrix, and placing in a refrigerator for freezing; spinning nanofibers onto stainless steel shaft core of a mold, putting whole nanofiber and the shaft core into a vacuum drying oven for drying; sheathing the outer layer of the nanofiber with the tubular matrix as a mechanical reinforcing layer; and assembling the compounded double-layer stent together with the shaft core into the mold, and performing solution casting and treatment to obtain the tubular stent. The prepared tubular stent has good mechanical performance, bursting strength and biocompatibility, has no hypersensitivity on a human body in transplanting operation, and has affinity with the blood and peripheral tissues in a human body, thus having good application prospects in cardiovascular and cardiac repair surgeries.

The invention provides hydrogel with ROS response and a preparation method and application thereof, during preparation, carbodiimide is used as a catalyst, catechol groups are grafted on chitosan macromolecules, and solubility and adhesion of chitosan are improved. Cystamine is used as a cross-linking agent, and amino of cystamine and benzoquinone formed after catechol groups are oxidized are subjected to Michael addition reaction to form a cross-linked network, so that the hydrogel is formed. The invention further discloses the hydrogel with ROS response prepared by the method and application of the hydrogel. The disulfide bond contained in cystamine can be broken and converted into sulfinic acid under the action of active oxygen, so that the structure of the hydrogel is destroyed, the hydrogel can play an intelligent drug release role in an atherosclerosis site where more active oxygen substances are generated, burst release of the drug in a diseased region is avoided, and risks of restenosis in blood vessels, delayed healing of endothelium, advanced thrombus and the like are expected to be reduced.

The invention utilizes gene recombination technology to construct a fusion protein (VEGF-Fc) of endothelial cell growth factor VEGF and Fc domain of immunoglobulin G. On the one hand, the fusion protein can better maintain the biological activity of VEGF and prolong its half-life; The specific affinity of endothelial cells can promote the adhesion and proliferation of endothelial cells and induce the directional differentiation of stem cells to endothelial cells, and improve the biological activity of anticoagulation and induction of angiogenesis of materials.

The invention relates to a plugging device and a preparation method thereof. The plugging device comprises a supporting net. The surface of the supporting net is coated with an anti-thrombus membrane,and the anti-thrombus membrane comprises an endothelialization promoting membrane. The endothelialization prompting membrane comprises a degradable polymer and endothelialization promoting matter dispersed in the degradable polymer. Or the endothelialization prompting membrane comprises the endothelialization promoting matter. The anti-thrombus membrane further comprises a controlled release membrane covering the endothelialization promoting membrane. The controlled release membrane comprises the degradable polymer. By means of the plugging device, thrombosis can be reduced or avoided.

The invention discloses an intravascular stent overlay film for in-situ intima regeneration and a preparation method of the intravascular stent overlay film. The method comprises the following steps:dividing silkworm natural silk into two groups, performing twisting and combining into silk thread groups, taking the other group as a monofilament group, performing degumming, performing dissolving in a lithium bromide neutral salt solution to prepare a silk fibroin dissolving solution, and then pouring the silk fibroin dissolving solution into a dialysis bag with a certain molecular weight cut-off; performing dialyzing to obtain a purified silkworm silk fibroin aqueous solution, and then concentrating the silk fibroin to a proper mass fraction by adopting a rotary evaporator; adding a hydrophilic flexible molecule cross-linking agent into the silk fibroin aqueous solution, performing uniform mixing, removing bubbles to obtain a modified silk fibroin solution, knitting monofilaments intoa tubular structure by adopting a knitting machine, performing coating to the fibroin tubular structure, performing rotary air drying on each coating in the circumferential direction under low-temperature hot air, and controlling the coating to be a uniform molecular layer, so as to obtain the intravascular stent overlay film for in-situ intima regeneration. The overlay film has excellent toughness, and in-situ induction of endovascular regeneration for regulating balance of a blood system is realized.

The invention relates to a composite drug stent and a preparation method thereof. The stent includes a stent body and active drugs; wherein, the stent body is a perforated medical material with good biocompatibility, which can be made by optionally using stainless steel, cobalt-based alloy, titanium alloy, nickel-titanium alloy, or polylactic acid bio-polymer material; the active drugs include special endothelial progenitor cell antibody drug and anti-smooth muscle cell proliferation drug. The internal surface of the perforated stent body is fixedly provided with the special endothelial cell antibody drug, while the external surface of the perforated stent body is coated with the anti-smooth muscle cell proliferation drug. The preparation method includes as follows: (1) pretreating the surface of the stent body; (2) making holes; (3) post-treating the surface of the stent body; (4) dispensing medicines; (5) coating the external surface; (6) fixing the internal surface; (7) drying at low temperature. The preparation method can selectively absorb endothelial progenitor cells and facilitate in repairing endodermis, and can effectively inhibit the proliferation and migration of vascular smooth muscle cells, persistently and effectively reduce the newborn endangium, effectively prevent the stent from narrowing, and avoid the risk of tardive thrombosis.

The invention discloses a spring ring. The spring ring comprises a spring ring body and a fiber bundle fixedly connected to the spring ring body. The spring ring has the following beneficial effects: according to the structure design of the provided spring ring, the applied friction force in a conveying pipe is lower while the spring ring is released; and the pushing force is more easily and better transmitted along the axial direction of the spring ring, and the stacking situation of the spring ring cannot be produced, so the operation difficulty of an operator is reduced, and the aneurysms rupture risk is reduced.

The invention relates to the field of interventional medical devices, and especially relates to a surface modification method for improving the corrosion resistance of a magnesium alloy intravascularstent. The method is suitable for a novel dual-drug degradable magnesium alloy intravascular stent for treating intraluminal stenosis, carrying a smooth muscle proliferation inhibitor and promoting endothelialization. The method is characterized in that a drug coating with an endothelialization promoting function is prepared on a magnesium alloy stent to accelerate the endothelialization process after stent implantation and improve the service environment of the stent, so the corrosion resistance of the magnesium alloy intravascular stent is improved. The magnesium alloy intravascular stent carries a drug for inhibiting intimal hypertrophy, and also carries a drug for promoting endothelialization. The stent can realize rapid endothelial regeneration of damaged parts through the action of the drug for promoting endothelialization after being implanted into a diseased blood vessel, so the service environment of the magnesium alloy stent is improved, and the corrosion resistance of the degradable magnesium alloy intravascular stent is improved.

The invention relates to the field of medical instruments, in particular to an aneurysm plugging device and a preparation method thereof. The aneurysm plugging device comprises a plugging device body of a net-shaped woven structure. A first binding part and a second binding part are formed at the two ends of the net-shaped woven structure respectively. The free end of at least one woven tow in the first binding part and the second binding part faces the interior of the net-shaped woven structure. The aneurysm plugging device is further provided with a far-end developing mark and a near-end developing mark, and the far-end developing mark and the near-end developing mark are arranged on the first binding part and the second binding part respectively. The aneurysm plugging device is high in metal coverage rate, can effectively reduce compression of an implantation device on aneurysm and interference of the implantation device on blood flow in a tumor cavity, and is beneficial to rapid endothelialization of cells.

The invention provides a woven reinforced degradable polyurethane elastomer artificial blood vessel and a preparation method thereof. The inner layer of the artificial blood vessel adopts activated H2N-PEG-COOH, heparin and (Mpa)-GGGGGREDV polypeptide to treat a C-PEEUU-NH2 electrospun nanofiber tubular stent to obtain a C-PEEUU-PEG-Hep / REDV tubular stent, the middle layer adopts a woven reinforced layer of nanofiber yarns woven by dissolving and mixing one or more master batches of PLGA, PDO and PGA according to a mass ratio, and the outer layer is a macroporous layer prepared by thermally induced phase separation. The woven reinforced degradable polyurethane elastomer artificial blood vessel provided by the invention can be used for tissue regeneration and construction of cardiovascularand cerebrovascular diseases, the preparation method is simple and efficient, the price is low, and the prepared artificial blood vessel has resilience, compliance, bursting strength and biocompatibility matched with autologous vascular tissues. The artificial blood vessel has no hypersensitivity to a human body in a transplantation operation, has affinity with blood and peripheral tissues in thehuman body, and has a good application prospect in repair operations of cardiology surgery, nephrology surgery and brain surgery.

The invention belongs to the technical field of medical instruments, and discloses a biological factor coating stent and a preparation method thereof. The biological factor coating stent comprises a Ni-Ti stent with a PDA coating, a DA stent with a heparin sodium coating, a DA-Hep stent with a VEGF coating, a DA-Hep stent with an anti-CD 34 antibody coating, a stent without heparin, and a stent simultaneously loaded with PDA, biological factors and heparin. Self-polymerization of dopamine DA is utilized to form polydopamine PDA, and an irreversible organic complex is formed on the metal surface and metal, so that the PDA is used as a connecting stent and a biological factor medium coating; and after a polydopamine PDA coating is constructed on the surface of the stent, heparin Hep, vascular endothelial growth factor VEGF and an anti-CD 34 antibody are sequentially loaded by utilizing Schiff base reaction and Michael addition, and the biological factor coating stent is obtained. Endothelial progenitor cells in blood circulation are effectively captured, and proliferation and migration of endothelial cells around the neck of an aneurysm are promoted.

The invention relates to a chondroitin sulfate modified collagen / polycaprolactone vascular repair scaffold and a preparation method and application thereof. According to the invention, coupling between chondroitin sulfate and collagen is realized by adopting a reductive amination method with a relatively mild reaction process, the chondroitin sulfate modified collagen obtained by chemical coupling is used as a base material of the scaffold, polycaprolactone is used as a mechanical reinforcing material, and the chondroitin sulfate modified collagen / polycaprolactone vascular repair scaffold is prepared by an electrostatic spinning method. The vascular repair scaffold has a good antithrombotic effect, can realize rapid endothelialization, is expected to be used as a vascular repair scaffold material, is applied to transplantation, repair and regeneration of small blood vessels, and is used for treating cardiovascular diseases which seriously threaten human health.

The invention relates to a method for preparing a P(LLA-CL) / collagen double-layer vascular stent coordinated and regulated by heparin and gemini, comprising: mixing P(LLA-CL) and collagen in a solvent to obtain a composite spinning Silk liquid; dissolving heparin sodium and VEGF in the diluent to obtain the drug solution loaded on the inner layer; dissolving PDGF in the diluent to obtain the drug solution loaded on the outer layer; the drug solution loaded on the inner layer is the core layer, and the spinning solution is the shell The inner layer of the stent is obtained by coaxial electrospinning; the drug solution loaded on the outer layer is the core layer, and the spinning solution is the shell layer, and the outer layer of the stent is obtained by bidirectional conjugate electrospinning, which is continuously received in the inner layer of the stent On the outside, a double-layer vascular stent was obtained, cross-linked, and obtained. The vascular stent of the present invention has excellent mechanical properties and biocompatibility, can mimic the components, structures and functions of natural blood vessels, and is beneficial to the in situ regeneration of vascular tissues and the reconstruction of multilayer structures, and is of great importance in vascular tissue engineering. Applications.

The invention discloses a preparation method of an artificial blood vessel, which comprises the following steps: preparing a polylactic acid solution by using polylactic acid as a raw material and hexafluoroisopropanol as a solvent; adding collagen sponge from marine organisms into the polylactic acid solution, and preparing a collagen sponge-polylactic acid composite solution; printing the artificial blood vessel at a platform temperature of -35 DEG C to-10 DEG C by adopting a biological 3D printer according to a file with a suffix name of stl output by cartographic software Auto CAD by applying the composite solution, and enabling the shape of the artificial blood vessel to meet the requirement of a target site of a human body to-be-implanted; and performing a cross-linking reaction on the interior of the material of the artificial blood vessel through glutaraldehyde steam. The preparation method is simple, the product is controllable, the prepared artificial blood vessel can be degraded in the human body, the use requirement is met, the replaceable artificial blood vessel can be customized for a patient according to the actual situation of the patient and the operation requirement, and the individuation level is high.

The invention discloses a covered stent, which comprises a support structure and a tectorial membrane unit, and is characterized in that: the tectorial membrane unit comprises an inner-layer tectorial membrane and an outer-layer tectorial membrane which coats the support structure; the outer-layer membrane coats on the outer side of the inner-layer tectorial membrane; the inner-layer tectorial membrane is a biological membrane with good blood compatibility, and the outer-layer tectorial membrane is a biological membrane which is easy to induce the formation of thrombus; the inner-layer tectorial membrane contacting blood has good blood compatibility, can effectively reduce the formation of the thrombus, and maintains normal blood flowing channels; and the outer-layer tectorial membrane can induce the formation of the thrombus in aneurysm or thrombus of bleeding parts, promotes the aneurysm to be quickly activated and reduced, reduces the mass effect in vivo, simultaneously effectivelyadheres endothelial cells, promotes differentiation and proliferation of cells, quickens the endothelialization of lesion parts of vessels, and can more effectively treat vascular diseases such as the aneurysm and the like.

The invention discloses a bilobalide B-sirolimus asymmetric compound drug eluting stent and a preparation method thereof. A bilobalide B-polylactic acid coating is coated on the inner surface the stent; and a bilobalide B-polylactic acid coating and a sirolimus-polylactic acid coating are sequentially coated on the outer surface of the stent. The preparation method comprises the following steps: 1, mixing bilobalide B with polylactic acid in a mass ratio of 1:(0.7-2) to obtain serous fluid for later use, and mixing sirolimus with polylactic acid in a mass ratio of 1:(0.7-2) to obtain serious fluid for later use; 2, spraying the mixed serous fluid of bilobalide B and polylactic acid to the inner and outer surfaces of the stent, and drying at a low temperature; and 3, roller-coating the mixed serous fluid of sirolimus and polylactic acid to the outer surface of the stent, and drying at a low temperature to obtain the bilobalide B-sirolimus asymmetric compound drug eluting stent. The asymmetric compound drug eluting stent disclosed by the invention has good effects of resisting thrombus, promoting vascular stent endothelialization and resisting restenosis, and can be used as a new-generation coronary medicament stent.