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Hydrogel with ROS response and preparation method and application thereof

A hydrogel and chitosan technology, applied in the field of ROS-responsive hydrogel and its preparation, can solve the problems of narrowness, poor water solubility of chitosan, and poor adhesion, so as to solve the problems of poor adhesion and water solubility. The effect of poor sex and prevention of restenosis

Active Publication Date: 2021-07-13
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention provides a ROS-responsive hydrogel and its preparation method and application, which introduces catechol groups, endows chitosan with a certain adhesion ability, and simultaneously acts as a fast Endothelialization, prevent restenosis, effectively solve the problems of poor water solubility and poor adhesion of chitosan

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  • Hydrogel with ROS response and preparation method and application thereof
  • Hydrogel with ROS response and preparation method and application thereof
  • Hydrogel with ROS response and preparation method and application thereof

Examples

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Embodiment 1

[0044] A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0045] (1) Catechol-modified chitosan is added in the Tris-base solution, magnetically stirred until fully dissolved, then stirred for 1h under ice-bath conditions to obtain an intermediate solution;

[0046] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0047] Catechol-modified chitosan is prepared by the following method:

[0048] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain chitosan solution;

[0049] (2) Dissolve 3,4-dihydroxyphenylpropionic acid in deioniz...

Embodiment 2

[0054] A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0055] (1) Catechol-modified chitosan is added in the Tris-base solution, magnetically stirred until fully dissolved, then stirred for 2h under ice-bath conditions to obtain an intermediate solution;

[0056] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0057] Catechol-modified chitosan is prepared by the following method:

[0058] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain chitosan solution;

[0059] (2) Dissolve 3,4-dihydroxyphenylpropionic acid in deioniz...

Embodiment 3

[0064] A kind of hydrogel with ROS response, its preparation method comprises the following steps:

[0065] (1) Catechol-modified chitosan is added in the Tris-base solution, magnetically stirred until fully dissolved, then stirred for 2h under ice-bath conditions to obtain an intermediate solution;

[0066] (2) Dissolve cystamine dihydrochloride in the Tris-base solution, then dropwise add it to the intermediate solution obtained in step (1) under stirring conditions, continue stirring for 5 minutes, and then place it at a temperature of 37°C to form a gel to obtain ROS-responsive hydrogels.

[0067] Catechol-modified chitosan is prepared by the following method:

[0068] (1) Add chitosan to RO water, then add concentrated hydrochloric acid and stir until completely dissolved, adjust the pH value to 5 by using 1mol / L sodium hydroxide solution and 1mol / L hydrochloric acid solution to obtain chitosan solution;

[0069] (2) Dissolve 3,4-dihydroxyphenylpropionic acid in deioniz...

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Abstract

The invention provides hydrogel with ROS response and a preparation method and application thereof, during preparation, carbodiimide is used as a catalyst, catechol groups are grafted on chitosan macromolecules, and solubility and adhesion of chitosan are improved. Cystamine is used as a cross-linking agent, and amino of cystamine and benzoquinone formed after catechol groups are oxidized are subjected to Michael addition reaction to form a cross-linked network, so that the hydrogel is formed. The invention further discloses the hydrogel with ROS response prepared by the method and application of the hydrogel. The disulfide bond contained in cystamine can be broken and converted into sulfinic acid under the action of active oxygen, so that the structure of the hydrogel is destroyed, the hydrogel can play an intelligent drug release role in an atherosclerosis site where more active oxygen substances are generated, burst release of the drug in a diseased region is avoided, and risks of restenosis in blood vessels, delayed healing of endothelium, advanced thrombus and the like are expected to be reduced.

Description

technical field [0001] The invention belongs to the technical field of cardiovascular treatment, in particular to a hydrogel with ROS response and its preparation method and application. Background technique [0002] The use of environment-responsive materials to control drug release is a current research hotspot, and it has been proven to have good effects in the field of targeted nanocarriers, various surface coatings, and hydrogels. In the research of environment-responsive hydrogels, the response mode is mainly reflected in the changes of quality, swelling ability and degradation ability according to the changes of the surrounding environment. Due to the presence of inflammation, the microenvironment of the atherosclerotic site has a weakly acidic pH (≈6.5, normal human body pH=7.4) and contains more reactive oxygen species, which makes it difficult for the preparation of intelligent environment-responsive hydrogels suitable for this environment. Glue provides the base....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/722A61K47/54A61P9/10C08J3/075C08B37/08C08L5/08
CPCA61K9/06A61K31/722A61K47/545A61K47/54A61P9/10C08J3/075C08B37/003C08J2305/08
Inventor 赵安莎刘力路槟阳杨苹
Owner SOUTHWEST JIAOTONG UNIV
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