77 results about "Cystamine Dihydrochloride" patented technology

The invention relates to polysaccharide hydrogel with double responses of PH and oxidation reduction as well as a preparation method and application of the polysaccharide hydrogel. The polysaccharide hydrogel is prepared from the following raw material components: a cystamine dihydrochloride solution and a cellulose acetoacetate solution. The preparation method comprises the following steps: under the condition of room temperature, adding the cystamine dihydrochloride solution into the cellulose acetoacetate solution, oscillating, uniformly mixing and standing to obtain a finished product. The preparation method disclosed by the invention has the advantages of simple process, safe operation, mild reaction conditions and suitability for most polysaccharide derivatives; meanwhile, cellulose is used as a natural green material, and has the characteristics of abundant resources, good biocompatibility and biodegradability. With unique responsiveness and biocompatibility, the hydrogel has good application potential and a broad market prospect in the field of drug carrier release.

The invention relates to a fluorescent probe for detecting the intracellular mercury ions, a synthesizing method thereof and the purposes. The fluorescent probe has a good selectivity on the mercury ions and also has good cell permeability; and the probe has no toxic side effects and is applied to the intracellular mercury ion detection. The method comprises the following steps: dissolving 1 mol of 3-carboxyl rhodamine into a dry organic solvent, slowly dripping 1-2 mol of POCl3 under stirring, heating, refluxing, removing the organic solvent and obtaining the crude rhodamine chloride; dissolving 1 mol of 2-aminoethanethiol hydrochloride or cystamine dihydrochloride in a dry organic solvent, adding 4-8 mol of acid binding agent, slowing dripping the obtained rhodamine chloride in ice bath, removing the organic solvent and obtaining the target product of the formula (I) or the formula (II), wherein, X is O; and dissolving the equivalent mol of the target product and the Lawson reagent in a dry organic solvent, heating and refluxing under the protection of inert gas, and finally obtaining the target product of the formula (I) or the formula (II) through column chromatography separation , wherein, X is S.

The invention discloses a biological functionalized gold nanorod molecular probe as well as a preparation method and application thereof. The preparation method comprises: cystamine dihydrochloride substitutes for cetyltrimethyl ammonium bromide (CTAB) on the gold nanorod, and EDC / NHS is coupled with specific biomolecules to obtain the biological functionalized gold nanorod molecular probe. The biological functionalized gold nanorod molecular probe has the advantages of stable fluorescence performance, favorable biocompatibility and simple surface functionalization process, has the functions of near-infrared absorption and fluorescence emission, can label cells or tissues in a specific way, and can emit fluorescence with different colors by regulating different excitation wavelengths, thereby realizing the goal of carrying out multicolor labeling by using a single gold nanorod molecular probe. The biological functionalized gold nanorod molecular probe has strong drift resistance, and is a novel fluorescent probe which can replace the traditional fluorochrome.

The invention discloses a responsive chitosan microsphere / cellulose hydrogel drug-loading composite membrane and preparation thereof. The preparation includes: preparing chitosan microspheres that load tetracycline hydrochloride which is an antibacterial drug; dispersing the prepared chitosan microspheres and 5-fluorouracil evenly in three-dimensional network structure formed by crosslinking carboxymethyl cellulose and cystamine dihydrochloride, wherein 5-fluorouracil is an anticancer drug. The responsive chitosan microsphere / cellulose hydrogel drug-loading composite membrane has good biocompatibility and reduction response, can release tetracycline hydrochloride and 5-fluorouracil in controlled manner in cancer tissue areas, and has antibacterial and anticancer functions.

The invention discloses a chemical synthesis method of valnemulin hydrochloride, which comprises the following steps of: taking refined pleuromutilin as raw material, carrying out sulfonation by paratoluensulfonyl chloride, and reacting with dimethyl cysteamine hcl, to obtain the pleuromutilin dimethyl cysteamine substitute; reacting D-valine, methyl acetoacetate and potassium hydroxide to obtain (R)-2-(1-methoxycarbonyl group-2-allyl) amino-3-methyl potassium butyrate, activating by ethyl chloroformate and reacting with the pleuromutilin dimethyl cysteamine substitute, adjusting PH value, carrying out reverse phase extraction, and carrying out freeze-drying to obtain the valnemulin hydrochloride. The method has the advantages that due to the refining of the raw material pleuromutilin, the impurities in the product can be effectively removed, and the purifying process can be simplified from the source; the carboxyl of D-valine can be activated by the ethyl chloroformate, so that the reaction is easier to carry out; and due to the pH adjustment, the reverse phase extraction, and the freeze-drying, the product can be obtained, so that the product is stable in quality, and high in purity.

The invention provides a magnetic material for efficiently detecting a circulating tumor cell and a preparation method of the magnetic material. The preparation method comprises the steps of: (1) allowing hyaluronic acid, 1-(3-dimethylamino propyl)-3-ethyl-carbodiimide hydrochloride, 1-hydroxybenzotriazole, cysteamine hydrochloride and dithiothreitol to react to prepare HA-SH, (2) grafting rhodamine onto HA-SH to prepare RhB-HA-SH, (3) allowing an Fe3O4 nanoparticle, RhB-HA-SH and H2O2 to react to prepare superparamagnetic fluorescent HA-SH, and (4) allowing superparamagnetic fluorescent HA-SH, 1-(3-dimethylamino propyl)-3-ethyl-carbodiimide hydrochloride, 1-hydroxybenzotriazole, PEG-FA and anti-EpCAM (epithelial cell adhesion molecule) to perform antibody reaction to prepare the magneticmaterial. The magnetic material is high in saturation magnetization, and good in magnetic response and biocompatibility, can achieve specific binding with the circulating tumor cell and has certain universality.

The invention relates to a method using alkaline hydrolysis to prepare cysteamine hydrochloride, in particular to synthesizing cysteamine hydrochloride in alkali surroundings, which is characterized in that: ethanolamine solution and sulfate solution are used as raw material; 2- amino ethyl sulfate is firstly synthesized before made into ring in alkaline solution by 2-amino ethyl sulfate and carbon disulfide, thereby getting Alpha -mercaptothiazoline; alkaline hydrolysis is made upon Alpha -mercaptothiazoline to produce cysteamine hydrochloride. The preparation method for cysteamine hydrochloride by using alkaline hydrolysis has the advantages of simple method, short production period and low production cost. The recovery rate of synthesizing Alpha -mercaptothiazoline reaches more than 90%. The cysteamine hydrochloride can be widely applied in manufacturing anti-ulcer drugs such as ranitidine and cimetidine, manufacturing animal fodder additive, manufacturing cosmetic and hair wave-setting agent, manufacturing biochemical reagent and heavy metal ion complexion agent and manufacturing agent treating radiation syndrome and acute tetraethyl lead poisoning and in other areas.

The invention discloses a compound which is 2[2-(1-ethanethiol)] urea-4 [1H]-pyrimidone. The invention also discloses a synthesis method of the compound, which comprises the steps: enabling cystamine dihydrochloride to react with activated ureido pyrimidine S1 to obtain a 2-urea-4[1H]-pyrimidine disubstituted sulfur-sulfur compound S2; and reducing the sulfur-sulfur compound S2 by using DTT (Dithiothreitol) to obtain the compound. The invention also discloses a method for synthesizing a double-UPy substituted compound by using the compound disclosed by the invention. According to the method, the compound has a free radical thiol-alkynyl click chemical reaction with an alkane alkynyl-terminated compound CHR to obtain the double-UPy substituted compound. According to the method disclosed by the invention, the thiol-alkynyl click chemical reaction is utilized to synthesize the double-UPy substituted compound for the first time; raw materials are easy to obtain, the application range is wide, the separation yield is high, and the synthesis method is scientific and reasonable; therefore a general method is provided for synthesizing the double-UPy substituted compound.

The invention discloses a preparation method of a crosslinking poly(p-phenylenebenzobisoxazole) film and belongs to the field of high polymer materials. The method comprises the steps that 2-methyl-4,6-diamido-resorcinol cystamine dihydrochloride and paraphthaloyl chloride are subjected to a reaction, and aromaticity polyamide with a methyl pendant group and a phenolic hydroxyl group is prepared; then the aromaticity polyamide is dissolved in a nonprotic polarity organic solvent to obtain a solution with a certain concentration, a substrate is coated with the solution, and heating is carried out to enable the solvent to evaporate to obtain a polyamide film; the polyamide film is treated at the high temperature to obtain the crosslinking poly(p-phenylenebenzobisoxazole) film. According to the method, extrusion forming is omitted, the presoma polyamide is dissolved in the organic solvent, the polyamide film with the certain thickness can be well prepared after the solvent is evaporated, the thickness of the crosslinking PBO film is controllable, and the film from nanoscale to microscale can be conveniently prepared. The preparation method can be applied to preparing photochromic devices, photovoltaic cells, organic light emitting diodes and other photoelectric devices, can also be used for a high-temperature resistance coating, and can well meet market requirements.

The invention discloses a synthetic method of dimethyl cysteamine hydrochloride. The method comprises the following steps of: synthesizing 5,5-dimethyl-2-isopropyl thiazoline by taking isobutylaldehyde, element sulfur, ammonia gas and triethylamine as raw materials; reducing the 5,5-dimethyl-2-isopropyl thiazoline into 5,5-dimethyl-2-isopropyl thiazolidine under the actions of sodium borohydride and acid; and reacting the 5,5-dimethyl-2-isopropyl thiazolidine and phenyl hydrazine under the air insulating situation to obtain dimethyl cysteamine hydrochloride. The method has the advantages of readily-available reaction raw materials, easiness for operating the reaction process, low requirement on the reaction equipment, relatively mild reaction conditions, feeding of the product of every step of reaction into a next step reaction after refining and purifying, high yield and high purity, and the purity of the finally-obtained dimethyl cysteamine hydrochloride is close to 100 percent.

The invention discloses a cysteamine hydrochlorate compound premix material for increasing the weight of meat poultry, and a method for preparing the same, wherein 10 to 30 portions of vitamin A, 3 to 10 portions of vitamin D3, 3 to 10 portions of vitamin E and one fifth to four fifths of 50 to 800 portions of xylo-oligosaccharide are evenly mixed, 0.5 to 1.5 portions of vitamin B2, 0.003 to 0.009 portion of vitamin B12, 0.50 to 1.0 portion of folic acid and one tenth to one fifth of 50 to 800 portions of xylo-oligosaccharide are evenly mixed, and the mixtures is evenly mixed with 100 to 900 portions of cysteamine hydrochlorate clathrate, 8 to 20 portions of methionine, 5 to 30 portions of lysine, 5 to 20 portions of solid emulsifier, 2 to 10 portions of glutamine and the remained carrier xylo-oligosaccharide to prepare the premix material. The compound premix material can supplement vitamins and amino acids needed for fast growth of meat poultry, strengthen the digestion, absorption and utilization of energy and nutrient substances such as nitrogen, calcium, phosphorus, and the like, improve the carcass merit, increase the meat factor, enhance the immunity function of organism, improve the illness resistant capability and the stress resistant capability, enforce the digestion and absorption of proteins and fats, improve the number of helpful bacteria in intestinal canals, enforce the digestion and absorption of nutrient substances and improve the feed conversion rate. The cysteamine hydrochlorate compound premix material can not only increase the producibility of meat poultry but also improve the meat quality.

The invention relates to a synthetic method of dimethyl cysteamine hydrochloride, and belongs to the technical field of organic synthesis. The method comprises the following processing steps: synthesizing 5,5-dimethyl-2-isopropyl thiazoline by taking isobutylaldehyde, element sulfur, ammonia gas and triethylamine as raw materials; reducing the 5,5-dimethyl-2-isopropyl thiazoline into 5,5-dimethyl-2-isopropyl thiazolidine under the actions of sodium borohydride and acid; and reacting the 5,5-dimethyl-2-isopropyl thiazolidine with a hydrochloric acid solution under nitrogen production to obtain the dimethyl cysteamine hydrochloride. The synthetic method has the advantages that the reaction raw materials are easily obtained; the reaction process is simple in operation; the requirement for reaction equipment is low; the reaction conditions are relatively mild; and the yield and content are high, and the content of the finally obtained dimethyl cysteamine hydrochloride is greater than 99%.

The invention relates to a viscous nanofiber hydrogel dressing capable of being peeled on demand and a preparation method thereof. An auxiliary material is prepared from raw materials containing a natural polymer material, a catechol compound, cystamine dihydrochloride and a coupling agent through electrostatic spinning and post-treatment. According to the method disclosed by the invention, nanofiber hydrogel is endowed with excellent mechanical strength and adhesion performance, and when the dressing is replaced or removed, the dressing can be easily stripped without pain by dropwise adding water on the adhesive dressing tightly attached to the skin, and secondary injury to a wound is avoided. The removed dressing is placed in the air to evaporate certain moisture, and when the moisture content of the dressing is about 150%, the dressing still has viscidity, so that repeated adhesion is achieved, and the service life of the dressing is prolonged.

The invention discloses a polyacrylic acid-S-S-drug copolymer and a preparation method thereof, and belongs to the fields of polymer chemistry and medicinal preparations. A drug derivative is preparedfrom a drug and cystamine dihydrochloride; and polyacrylic acid and the drug derivative are condensed to form the polyacrylic acid-S-S-drug copolymer. The polyacrylic acid-S-S-drug copolymer can spontaneously form an amphiphilic polymer micelle in an aqueous solution, the linkage bond is a disulfide bond, and the linkage bond can responsively rupture at the lesion to release the drug; and additionally, the polyacrylic acid can well improve the water solubility of the drug, and can be used to prepare a redox-sensitive polymer prodrug for improving the solubility of the poorly soluble drug. Theinvention also discloses a preparation method of the PAA-S-S-GA copolymer, and a use of the PAA-S-S-GA copolymer as an anticancer drug carrier.

The invention relates to a method for synthesizing 2,2-dimethyl cysteamine hydrochloride. The method comprises the steps that ketone or aldehyde, ammonium hydroxide and dimethyl ethylene sulfide are subjected to ring closing to obtain 5,5-dimethyl-2-substitued thiazolidine, and the 5,5-dimethyl-2-substitued thiazolidine is subjected to ring opening under the effect of hydrochloric acid to obtain 2,2-dimethyl cysteamine hydrochloride. The method has the advantages that a midbody does not need to be purified and can be put to a next reaction only through simple liquid separation operation, no phenylhydrazine is adopted in a reaction, the reaction time is greatly shortened, and the capacity is greatly improved. The raw materials of the reaction are easy to obtain, simple unit operations are adopted in the technology, the requirement for reaction equipment is low, the reaction conditions are mild, the yield and content are high, and the method is suitable for industrial production, and the content of the 2,2-dimethyl cysteamine hydrochloride is higher than 99%.

The invention relates to a preparation method of polyaniline-loaded gamma-PGA (Propylene Glycol Alginate) nano-hydrogel. The preparation method comprises the following steps: carrying out double emulsification reaction on EDC (Dichloroethane)-activated gamma-PGA to obtain a W / O / W polymer emulsion; dissolving cystamine dihydrochloride into ultrapure water, then dropwise adding a mixed solution into the W / O / W polymer emulsion, and reacting to obtain gamma-PGA / Cys nano-hydrogel; dissolving the gamma-PGA / Cys nano-hydrogel into ultrapure water with a pH value of 3.00, then adding the cystamine dihydrochloride, standing in an ice bath for 44 minutes to 1 hour, then adding ammonium persulfate for reacting, dialyzing and purifying to obtain a finished product. The preparation method disclosed by the invention has the advantages of simple process, easiness in operation and separation, wide source of raw materials and low cost; the prepared gamma-PGA hydrogel is uniform in particle size distribution and has the advantages of good water solubility, colloidal stability, biocompatibility, no adverse influence on a living body and potential application value in the field of photothermal therapy.

The invention discloses a degradable self-repairing conductive hydrogel, a preparation method and application thereof. The preparation method comprises the following steps: modifying cystamine dihydrochloride (CSA) on a branched chain of hyaluronic acid (HA) through an amido bond to obtain cystamine dihydrochloride-hyaluronic acid (CSA-HA); modifying pyrrole-1-propionic acid (Py) on CSA of CSA-HA through an amido bond to obtain pyrrole-1-propionic acid-cystamine dihydrochloride-hyaluronic acid (Py-CSA-HA); and soaking in a ferric chloride solution after the Py-CSA-HA is self-formed into gel, so that Py is polymerized into polypyrrole (PPy), and the degradable self-repairing conductive hydrogel is obtained. The method provided by the invention is simple and effective, the operation is simple and convenient, and the hydrogel has self-repairing, degradable and conductive functions, so that the hydrogel has better nerve repairing performance and electrical signal conduction performance when being applied to the aspect of nerve repairing biological tissue materials, especially conductive nerve scaffold materials.

The invention discloses a pig feed for improving the pork quality and a preparation method of the pig feed. The pig feed comprises the following components in part by weight: 20-70 parts of corn, 1-40 parts of bean pulp, 3-20 parts of wheat bran, 0.01-0.1 part of enveloped mercaptamine, 0.1-0.5 part of selenomethionine, 2-10 parts of big red bean powder, 5-30 parts of rice bran, 1-4 parts of a vitamin premix and 1-4 parts of a mineral element premix. The reasonable amount of enveloped mercaptamine, selenomethionine and big red bean powder are added in the feed, so that the prepared pig feed improves the daily gain and the feed intake of a pig, the feed conversion ratio of the fattening pig is reduced, the conversion rate of the feed is improved, the cooking loss and the malondialdehyde content of the pork are reduced, the inoxidizability of the pork is improved, and therefore, the meat quality and the meat color of the pork are effectively improved, and the shelf life of the pork is prolonged.

The invention discloses a preparation method and application of amino modified Fe3O4 nano microspheres. By taking ferric trichloride hexahydrate, anhydrous sodium acetate, polyethylene glycol, cysteamine hydrochloride (CSH) and ethylene glycol as raw materials, the amino-modified Fe3O4 nano microspheres (Fe3O4-NH2) are prepared. Okadaic acid in scallops is extracted by using the Fe3O4-NH2 as an adsorbent. Extraction conditions of the Fe3O4-NH2 on okadaic acid are optimized; and when the pH value is 7, the mass of the adsorbent is 20 mg, the vortex adsorption time is 20 min, the eluent is a mixture of ammonia water and methanol in a ratio of 10%: 90%, and the volume of the eluent is 4 mL, the extraction efficiency of the Fe3O4-NH2 on the okadaic acid in shellfish is optimal.

The invention discloses a sodium alginate-based nitric oxide donor as well as a synthesis method and application thereof. The synthesis method comprises the following steps: dissolving sodium alginate in ultrapure water, adding an activating agent for reaction, adding L-cysteine hydrochloride or cystamine dihydrochloride for continuous reaction; and dialyzing the reaction solution, adding tert-butyl nitrite, continuously reacting, dialyzing the obtained solution, and freeze-drying to obtain the sodium alginate nitric oxide donor. The sodium alginate nitric oxide donor synthesized by the method is S-nitrosylated sodium alginate, sulfydryl is grafted on sodium alginate, SNO groups are formed through nitrosation, the synthesis method is simple and easy to implement, non-toxic and low in cost, and the prepared nitric oxide donor is long in release time, can exist stably, and can be used for preparing the sodium alginate nitric oxide donor. And the material and other polymer materials are expected to be applied to preparation of multifunctional nitric oxide release composite materials.

The invention discloses an expanded silk wadding quilt based on mulberry silk and a preparation method of the expanded silk wadding quilt. The preparation method comprises the following steps: addinga mixed solution of turmeric extract, acetic acid and carboxymethylcellulose into a degummed silk fiber solution, performing ultrasonic treatment, adding polyurea-formaldehyde and selenocysteine hydrochloride, and performing ultrasonic treatment; preparing perforated silk into silk floss pieces, and impregnating in an aqueous solution of amino polyethylene glycol so as to obtain treated silk flosspieces; putting the treated silk floss pieces into a rotatable closed container filled with a rupture disk, and expanding to obtain expanded wet silk floss pieces; dehydrating the expanded wet silk floss pieces, manually garneting, and drying to obtain expanded silkworm dried silk floss pieces; and combining and sewing the expanded silkworm dried silk floss pieces and treated tussah silk floss pieces, thereby obtaining the expanded silk wadding quilt. The porous expanded silk floss pieces with excellent service performance are successfully prepared, and the expanded silk wadding quilt has excellent antibacterial property and also has positive practical significance.

The invention discloses a goose down based expanded silk wadding quilt and a preparation method thereof. The preparation method of the goose down based expanded silk wadding quilt comprises the following steps: adding a mixed solution of turmeric extract, vinegar and carboxymethylcellulose to a degummed silk fibroin solution for ultrasonic treatment, and adding polyureaformaldehyde and selenocystamine dihydrochloride for ultrasonic treatment; preparing silk wadding sheets from silk with holes and impregnating the silk wadding sheets in an amino polyethylene glycol aqueous solution to obtain treated silk wadding sheets; putting the treated silk wadding sheets in a rotatable closed container filled with a rupture film for expansion to obtain the expanded wet silk wadding sheets; dehydratingand unloosening the expanded wet silk wadding sheets manually and drying the same to obtain the expanded dry silk wadding sheets; and combining and sewing the expanded dry silk wadding sheets with thetreated goose down to obtain the expanded silk wadding quilt. The porous expanded silk wadding sheets which are excellent in wearability are prepared successfully and have good bacterial resistance,and the preparation method has realistic positive meaning.

The invention discloses a dithiophospholipid compound and a preparation method thereof. The preparation method comprises the following steps: weighing cystamine hydrochloride and triethylamine absolute methanol, adding di-tert-butyl dicarbonate, and reacting to obtain single protection cystamine; weighing fatty acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxythiosuccinimide, dissolving in dichloromethane, adding mono-protected cystamine, mixing, dissolving reactants in dichloromethane, and adding trifluoroacetic acid to obtain a solid; dissolving the solid in dichloromethane, adding triethylamine and succinic anhydride, and reacting to obtain an intermediate product; and weighing the intermediate product according to the ratio, dissolving dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dimethyl sulfoxide, activating, adding choline glycerophosphate, and reacting to obtain the target product. The structure of the dithiophospholipid compound provided by the invention contains a disulfide bond which is easily reduced by glutathione, so that phospholipid is broken in the presence of GSH, and the dithiophospholipid compound has a GSH response breaking function.

The invention provides a nano-drug and a synthesis method thereof and belongs to the technical field of drug synthesis. The synthesis method of the nano-drug comprises the following steps: (1) taking2,2'-bipyridinemethylamine and alpha,alpha'-dichloro para-xylene to react to obtain a monomer; (2) taking cystamine dihydrochloride and triethylamine to react to obtain cystamine, and then adding themonomer to obtain a first polymer; (3) taking hyaluronic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to react, and then adding a mixed solution of the triethylamine and the first polymer; then reacting to obtain a second polymer; (4) after dissolving the second polymer into a buffering solution, adding a chelating agent containing Zn<+> and reactingto obtain a third polymer; mixing the third polymer and small fragment interfered RNA (Ribonucleic Acid) and incubating under the condition of 55 to 65 DEG C to obtain the nano-drug. The nano-drug obtained by the synthesis method has a better treatment effect on malignant glioblastoma multiforme.

The invention discloses a finishing process of a denim fabric. The finishing process comprises the following steps of (1) successively adding 1-2 parts by weight of sodium alpha-olefinsulfonate, 3.8 parts by weight of chitosan propionic acid, 0.8 part by weight of natamycin and 1.3 parts by weight of isothiazolinone into 20 parts by mass of deionized water, and uniformly stirring the raw materials; (2) then at the same time, adding 0.9 part by weight of realgar, 1.2 parts by weight of cystamine dihydrochloride, 0.7 part by weight of benzenedicarboxylic acid dimethyl ester and 0.2 part by weight of dibutyltin dilaurate, and continuously stirring until sufficient and uniform mixing is achieved, to obtain a finishing liquid; and (3) carrying out dipping treatment of the fabric in the finishing liquid, and drying at constant temperature after taking out the fabric. According to the finishing process of the denim fabric, the finished fabric has the advantages of excellent antibacterial, mildew-proof, flame-retardant, anti-moth and anti-static performance, strong washing resistance, and soft hand feeling.

The invention discloses a synthesis method of a MoS2 QDs fluorescent probe. The synthesis method comprises following steps: (1) dissolving cystamine dihydrochloride and ammonium tetrathiomolybdate into deionized water to obtain a mixed solution with a pH value of 7 to 11; (2) processing the mixed solution by ultrasonic waves, and then heating the mixed solution to carry out reactions; (3) after reactions, making the system to stand still, and subjecting the upper layer turbid liquid to centrifugation; (4) after centrifugation, filtering the upper layer supernate to obtain a filtered solution;(5) subjecting the filtered solution to dialysis, and carrying out vacuum drying to obtain MoS2 QDs powder; (6) preparing a water solution of MoS2 QDs from the MoS2 QDs powder obtained in the step (5), wherein the MoS2 QDs powder water solution is used as a MoS2 QDs fluorescent probe for detecting heme. The invention also discloses a MoS2 QDs fluorescent probe and applications thereof. The provided MoS2 QDs fluorescent probe has the advantages of good biocompatibility, high stability, high fluorescence quantum yield, simple synthesis conditions, and low price. When the fluorescent probe is used to detect heme, the detection process is simple, and the sensitivity is high.