Extraction and purification method and application of fermented astragalus polysaccharide

A purification method, technology of astragalus polysaccharides, applied to medical preparations containing active ingredients, digestive system, pharmaceutical formulas, etc., can solve problems such as increasing the yield of fermented astragalus polysaccharides, reduce complex operation steps, facilitate full development and high efficiency Utilization, the effect of easy separation and extraction

Inactive Publication Date: 2015-01-14
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Does pH change alter the content of fermented astragalus polysaccharides? Many enzymes secreted by non-lactolytic streptococcus LZMYFGM9 can participate in the biotransformation process. Can cellulase extraction help increase the yield of fermented astragalus polysaccharides? There is no comparative study on the extraction methods of biotransformation TCM polysaccharides

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  • Extraction and purification method and application of fermented astragalus polysaccharide
  • Extraction and purification method and application of fermented astragalus polysaccharide
  • Extraction and purification method and application of fermented astragalus polysaccharide

Examples

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Embodiment 1

[0048] In this example, the bacterial species co-fermented with Astragalus is a non-lactolytic streptococcus isolated from chicken intestines ( Streptococcus alactolyticus ) LZMYFGM9, its deposit number is CGMCC No.4227. Preservation date: October 19, 2010; Preservation unit: General Microbiology Center of China Microbiological Culture Collection Management Committee; Preservation unit abbreviation: CGMCC; Preservation unit address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; the strain See Chinese patent document ZL 201210141827.5 (name of invention: a non-lactolytic streptococcus and its application) for its culture characteristics.

[0049] In this example, the determination of the protein content in the fermented astragalus polysaccharides was carried out by using the BCA protein concentration determination kit of Suleibao Company.

[0050] The mass fraction of crude drug Radix Astragali in the present embodiment fermented Radix Astragali is...

Embodiment 2

[0063] The extraction and purification of crude drug astragalus polysaccharide includes the following steps:

[0064] (1) Weigh 312 g of crude drug Astragalus root powder (passed through a 20-mesh sieve), soak in 20 times the amount (6240 mL) of 85% ethanol at room temperature for 8 h, soak at 85 °C for 1 h, filter, and extract the residue once more . The dregs were dried in a blast drying oven at 50 ℃ until there was no alcohol smell and used to extract the crude drug astragalus polysaccharide.

[0065] (2) Add 10 times the amount (mL / g) of pure water to the dregs obtained in step (1), soak at room temperature for 8 h, then soak in a water bath at 85 °C for 1 h, and pour out the supernatant. Repeat this step two more times. The three supernatants were combined, after centrifugation, the supernatant was concentrated to 1:5 using a Sartorius Vivaflow 200 ultrafilter with a molecular weight cut-off of 5000 Dal, and then assisted by heating in a water bath at 90 °C to concentra...

Embodiment 3

[0077] The difference between this embodiment and embodiment 1 is:

[0078] (1) Weigh 400 g of the fermented astragalus freeze-dried powder prepared in Example 1 (through a 20-mesh sieve), soak in 20 times the amount (8000 mL) of ethanol with a volume percentage of 85% at room temperature for 9 hours, soak at 85°C for 1 hour, and filter , and the filter residue was extracted once more. The dregs are dried in a blast drying oven at 50°C until there is no alcohol smell and used to extract fermented astragalus polysaccharides.

[0079] (2) Add 15 times the amount (mL / g) of pure water to the dregs obtained in step (1), soak at room temperature for 9 hours, then soak in a water bath at 85°C for 1 hour, and pour out the supernatant. Repeat warm immersion and extraction of the filter residue twice: add 15 times the amount (mL / g) of pure water to the medicinal residue, soak at room temperature for 9 hours, then soak in a water bath at 85°C for 1 hour, and pour out the supernatant.

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Abstract

The invention provides an extraction and purification method and application of a fermented astragalus polysaccharide. The extraction and purification method of the fermented astragalus polysaccharide comprises the following steps of (1) carrying out warm immersion extraction on fermented astragalus, concentrating an extracting solution by using a membrane intercepting ultrafilter, removing protein through a Sevag method, and carrying out dialysis, alcohol precipitation and washing to obtain a crude fermented astragalus polysaccharide; (2) purifying the fermented astragalus polysaccharide by adopting DEAE Sepharose FastFlow, eluting by using ultrapure water, concentrating and freeze-drying an eluant to obtain a neutral polysaccharide DF-1, eluting by using gradient NaCl, and concentrating, dialyzing, concentrating and freeze-drying the eluant to obtain a weakly acidic polysaccharide; (3) purifying the neutral polysaccharide DF-1 obtained from the step (2) by adopting a Sephacryl S-400 High Reslution preparation chromatographic column, eluting by using ultrapure water, concentrating and freeze-drying the eluant to obtain neutral polysaccharides SF-1 and SF-2. Compared with a crude drug APS, the FAPS (Fermented Astragalus Polysaccharide) extracted by applying the extraction and purification method provided by the invention is outstandingly increased in yield and content.

Description

technical field [0001] The invention relates to a method for extracting and purifying fermented astragalus polysaccharide and its application. Background technique [0002] With the development of modern science and technology and the deepening of the modernization of traditional Chinese medicine, the application of fermentation engineering to the research and development of traditional Chinese medicine preparations has been frequently reported. Some scholars have used different bacterial species to biotransform the traditional Chinese medicine Astragalus membranaceus by solid and liquid fermentation methods. The research results show that the yield and efficacy of polysaccharides and polyphenols of Astragalus membranaceus have been significantly improved after biotransformation. [0003] Regarding the separation and purification methods of biological polysaccharides, there are various reports. The traditional process generally adopts water decoction. Saturated lime water e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
Inventor 秦哲李建喜杨志强王学智张景艳王旭荣王磊孔晓军孟嘉仁张凯
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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