Method for removing pigment in spinosad fermented liquid

A technology of spinosyn and fermentation liquid, which is applied in the direction of separation methods, chemical instruments and methods, biocides, etc., can solve the problems that are not conducive to process scale-up production, cannot achieve complete adsorption, and the filtrate is turbid, and achieve fast adsorption speed, Strong adsorption capacity and the effect of improving the recovery rate

Inactive Publication Date: 2011-12-07
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This resin leaks when it adsorbs spinosad at PH5-11, it cannot achieve complete adsorption effect, and it also has high requirements on the adsorption rate, which is not conducive to the scale-up production of the process
[0005] The spinosad fermentation liquid has many impurities and the filtrate is turbid. At present, there is no simple method in the prior art that can effectively remove the pigment of the fermentation liquid and raise the purity of the spinosad to a higher level.

Method used

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  • Method for removing pigment in spinosad fermented liquid
  • Method for removing pigment in spinosad fermented liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Medium formula: carbon source - glucose nitrogen source - cottonseed meal

[0038] Take 3L of spinosad fermentation broth, centrifuge at 4000r / min for 30min to obtain mycelia, soak in 3L of acetone overnight, centrifuge again at 4000r / min for 30min to get the supernatant, and obtain the pretreated spinosad fermentation broth.

[0039] Take 500ml of the pretreated spinosad fermentation broth, the unit is 823ug / ml, and the pH is 6.0, select a glass chromatography column with a height of 20cm and a diameter of 3cm, fill it with acidic alumina about 10cm evenly, and leaching with acetone A certain amount of deionized water was added to the solution to prepare a 9:1 acetone aqueous solution and then loaded on the column, and the flow rate was controlled to be 15ml / min. After passing 500ml of the leaching solution through the column, continue to use 500ml of 9:1 acetone water to drain the column. Wash down the spinosad on and collect all eluate.

[0040] It was found that th...

Embodiment 2

[0042] Medium formula: carbon source - glucose nitrogen source - cottonseed meal

[0043] Take 3L of spinosad fermentation broth, centrifuge at 4000r / min for 30min to obtain mycelia, soak in 3L of acetone overnight, centrifuge again at 4000r / min for 30min to get the supernatant, and obtain the pretreated spinosad fermentation broth.

[0044] Take 500ml of the pretreated spinosad fermentation broth, the unit is 823ug / ml, the pH is 6.4, select a glass chromatography column with a height of 20cm and a diameter of 3cm, fill it with acidic alumina about 10cm evenly, and leaching with acetone The liquid was directly loaded on the column, and the flow rate was controlled at 15ml / min. After passing through the column, the spinosad on the column was continued to be washed with acetone, and all eluents were collected.

[0045]It was found that the eluate had become clear, almost colorless, most of the pigments had been removed, the purity was increased from 19.4% to 66%, and the yield w...

Embodiment 3

[0047] Medium formula: carbon source - glucose nitrogen source - cottonseed meal

[0048] Take 3L of spinosad fermentation broth, centrifuge at 4000r / min for 30min to obtain mycelia, soak in 3L of acetone overnight, centrifuge again at 4000r / min for 30min to get the supernatant, and obtain the pretreated spinosad fermentation broth.

[0049] Get pretreated spinosad fermented liquid 500ml, unit is 823ug / ml, and pH is 6.5, selects a height to be 20cm, the glass chromatography column of diameter 3cm, evenly fills in about 10cm of acid alumina, is made into 4: 1 of the acetone aqueous solution was put on the column, and the flow rate was controlled at 15ml / min. After passing 500ml of the leaching liquid through the column, continue to wash off the spinosad on the column with 500ml of 4:1 acetone water, and collect all the eluted liquid.

[0050] It was found that the eluate had become clear, had almost no color, removed most of the pigments, and the purity was increased to 68% fr...

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Abstract

The invention discloses a method for removing pigments in spinosad fermented liquid. The method comprises the steps of adsorbing and eluting the pretreated spinosyn fermented liquid on an alumina column. The pigment in the spinosyn eluate obtained by the method is basically removed, and the purity and yield are both improved. The whole process is simple and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of biopesticide production, and in particular relates to a method for removing pigments in spinosad fermentation liquid. Background technique [0002] Spinosad is a novel macrolide ultra-high-efficiency biological source extracted from the fermentation broth of Saccharopolyspora spinosa isolated from the soil of the Virgin Islands in 1982 by researchers from the American Lilly Company. pesticides. This type of compound not only has high biological activity, but also has low toxicity to non-target organisms, and is very safe to humans and other mammals. In the isolated extract of natural spinosad, the mixture of the two highest active components A and D is called spinosad, and the Chinese common name is spinosad, which is the main component of commercial products. Spinosad is mainly used in the control of cotton bollworm, tobacco budworm, diamondback moth, fruit fly, beet armyworm, thrips, etc. It is suitab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C07H1/06B01D15/00A01P7/00B01J20/08
Inventor 卢亮李继安韩丽敏
Owner SHANGHAI INST OF PHARMA IND CO LTD
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