Granular microbial ecological agent capable of improving productivity of ruminants and preparation method thereof
A microbial preparation, production performance technology, applied in microorganism-based methods, biochemical equipment and methods, molding or processing of animal feed, etc., can solve problems such as loss of activity, inability to mix with pellet feed, etc.
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Embodiment 1
[0034] Example 1: Preparation of granular probiotics that can improve the production performance of ruminants
[0035] 1. Formula: 30 parts of solid culture of Saccharomyces cerevisiae, 20 parts of solid culture of Aspergillus niger, 20 parts of solid culture of Aspergillus oryzae, 30 parts of solid culture of lactic acid bacteria, Bacillus subtilis powder.
[0036] Among them, the solid culture of Saccharomyces cerevisiae is produced by solid fermentation of Saccharomyces cerevisiae BLNJ03 with a preservation number of CCTCC NO: M 2015209;
[0037] The method of solid fermentation production is: the liquid seed of Saccharomyces cerevisiae (Saccharomyces cerevisiae) BLNJ03 is inoculated in the solid fermentation medium by the inoculum size of 5% (volume fraction), and fermentation temperature is 30 ℃, and aerobic fermentation time is 30h, anaerobic The oxygen fermentation time is 80h.
[0038] The solid fermentation medium is composed of substrate and water at a ratio of 1g:0...
Embodiment 2
[0051] Example 2: Preparation of granular probiotics that can improve the production performance of ruminants
[0052] Formula: 25 parts of solid culture of Saccharomyces cerevisiae, 25 parts of solid culture of Aspergillus niger, 15 parts of solid culture of Aspergillus oryzae, 35 parts of solid culture of lactic acid bacteria, and Bacillus subtilis powder.
[0053] Wherein, the production method of Saccharomyces cerevisiae solid culture, Aspergillus niger solid culture, Aspergillus oryzae solid culture, lactic acid bacteria solid culture and Bacillus subtilis bacterial powder is the same as embodiment 1.
[0054] The preparation method is the same as in Example 1, and the number of live bacteria of Bacillus subtilis in the prepared granular probiotics is 5.10×10 8 cfu / g.
Embodiment 3
[0055] Example 3: Preparation of granular probiotics that can improve the production performance of ruminants
[0056] Formula: 35 parts of solid culture of Saccharomyces cerevisiae, 15 parts of solid culture of Aspergillus niger, 25 parts of solid culture of Aspergillus oryzae, 25 parts of solid culture of lactic acid bacteria, and Bacillus subtilis powder.
[0057] Wherein, the production method of Saccharomyces cerevisiae solid culture, Aspergillus niger solid culture, Aspergillus oryzae solid culture, lactic acid bacteria solid culture and Bacillus subtilis bacterial powder is the same as embodiment 1.
[0058] The preparation method is the same as in Example 1, and the number of live bacteria of Bacillus subtilis in the prepared granular probiotics is 5.60×10 8 cfu / g.
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