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Fluorescent carbon quantum dot having peroxidase catalytic activity and preparation method of same

A technology of peroxidase and carbon quantum dots, applied in the field of nanomaterials, can solve problems such as lack of catalytic activity, and achieve the effects of high reproducibility, easy procurement, and simple preparation methods

Inactive Publication Date: 2018-01-19
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carbon quantum dots synthesized from natural carbon sources generally have no obvious catalytic activity.

Method used

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  • Fluorescent carbon quantum dot having peroxidase catalytic activity and preparation method of same
  • Fluorescent carbon quantum dot having peroxidase catalytic activity and preparation method of same
  • Fluorescent carbon quantum dot having peroxidase catalytic activity and preparation method of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) At room temperature, take 1g of pig blood and put it into a mortar to grind it into a paste, add it to a 50mL small beaker, add 10mL of deionized water, mix well, put it into an ultrasonic instrument and continue to sonicate for 20 minutes to make it well mixed.

[0027] 2) Take out the above suspension and add it into a 20mL autoclave, and heat it in a constant temperature drying oven. The reaction temperature was set at 180° C. for 8 hours. After the reaction was completed, it was naturally cooled to room temperature.

[0028] 3) Pour the above reaction solution into a syringe equipped with a 0.22 μm filter membrane and filter to remove the residue. Then the obtained filtrate was put into a centrifuge tube, and centrifuged at a speed of 8000 rpm for 10 minutes to remove insoluble matter.

[0029] 4) The supernatant was then rotary-evaporated to increase the concentration of the carbon quantum dot solution, and then freeze-dried for 24 hours to obtain a fluorescen...

Embodiment 2

[0037] 1) At room temperature, take 1g of sheep blood and put it into a mortar to grind it into a paste, add it to a 50mL small beaker, add 10mL of deionized water, mix well, put it into an ultrasonic instrument and continue to sonicate for 20 minutes to make it well mixed.

[0038] 2) Take out the above suspension and add it into a 20mL autoclave, and heat it in a constant temperature drying oven. The reaction temperature was set at 200° C. for 6 hours. After the reaction was completed, it was naturally cooled to room temperature.

[0039] 3) Pour the above reaction solution into a syringe equipped with a 0.22 μm filter membrane and filter to remove the residue. Then the obtained filtrate was put into a centrifuge tube, and centrifuged at a speed of 8000 rpm for 10 minutes to remove insoluble matter.

[0040] 4) The supernatant was then rotary-evaporated to increase the concentration of the carbon quantum dot solution, and then freeze-dried for 24 hours to obtain a fluoresc...

Embodiment 3

[0043] 1) At room temperature, take 1g of chicken blood and put it into a mortar to grind it into a paste, add it to a 50mL small beaker, add 10mL of distilled water, mix well, put it into an ultrasonic instrument and continue to sonicate for 20 minutes to make it evenly mixed .

[0044] 2) Take out the above suspension and add it into a 20mL autoclave, and heat it in a constant temperature drying oven. The reaction temperature was set at 160° C. for 10 hours. After the reaction was completed, it was naturally cooled to room temperature.

[0045] 3) Pour the above reaction solution into a syringe equipped with a 0.22 μm filter membrane and filter to remove the residue. Then the obtained filtrate was put into a centrifuge tube, and centrifuged at a speed of 8000 rpm for 10 minutes to remove insoluble matter.

[0046] 4) The supernatant was then rotary-evaporated to increase the concentration of the carbon quantum dot solution, and then freeze-dried for 24 hours to obtain a fl...

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Abstract

The invention discloses a fluorescent carbon quantum dot having peroxidase catalytic activity and a preparation method of same. The preparation method includes the following steps: 1) grinding animalblood, (such as hog blood, sheep blood, cattle blood, chicken blood and the like) which are purchased form market, in a mortar into paste, adding deionized water to prepare a turbid liquid, performingultrasonic treatment for 20 min, and adding a less amount of the turbid liquid into a high-pressure reaction kettle to perform a hydrothermal reaction; 2) after the reaction finished, cooling a reaction product to room temperature, filtering the reaction product to remove residues through a filter membrane being of 0.22 [mu]m, and centrifuging a filtrate to remove non-soluble precipitates; 3) performing rotary evaporation to a supernatant to increase the concentration of the carbon quantum dot solution, and freeze-drying the solution to obtain blue fluorescent carbon quantum dots which have strong peroxidase catalytic activity. The synthesized carbon quantum dot powder can be stored for a long time and can be blended according to actual concentration demand when being used. The preparation method has simple operation process and low cost. The carbon quantum dot is high in fluorescence yield, can be directly used in the fields of cell and biological imaging, bio-detection, catalysis, etc.

Description

technical field [0001] The invention relates to a fluorescent carbon quantum dot with peroxidase catalytic activity and a preparation method thereof, belonging to the field of nanometer materials and the field of preparation technology. Background technique [0002] Carbon quantum dots are a new type of fluorescent carbon nanomaterials that have only appeared in recent years. It is a zero-dimensional carbon nanocrystal with a diameter of less than 10nm and has good biocompatibility. This nanomaterial overcomes some shortcomings of traditional II-IV quantum dots, and has non-toxicity, high fluorescence quantum yield, good biocompatibility, high light and chemical stability, etc., and can be used for biosensing and bioimaging , environmental detection, photocatalysis and drug carriers and many other fields. Carbon quantum dots have the advantage of being able to replace biologically toxic semiconductor quantum dots, especially in the field of life sciences. [0003] There ar...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
Inventor 王斌丁艾玲郑九尚
Owner SOUTHWEST UNIVERSITY
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