Method for extracting immunoglobulin G from swine blood
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A technology of immunoglobulin and pig blood, applied in the field of extracting immunoglobulin G, to achieve the effects of shortening the production cycle, improving product appearance and reducing production costs
Inactive Publication Date: 2012-10-03
天津宝迪农业科技股份有限公司
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Problems solved by technology
[0006] The purpose of the present invention is to overcome the defects of the current ammonium sulfate fractional precipitation method for ex...
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Embodiment 1
[0019] Embodiment 1: a kind of method for extracting immunoglobulin G from pig blood, comprises the following steps:
[0020] 1. Collect healthy pig blood, and after anticoagulant treatment, the plasma is separated by a tubular separator. Healthy pig blood means that all pigs come from designated large-scale slaughterhouses, and must pass the inspection of the national animal quarantine department to be free of diseases, and additives such as melamine and "lean meat powder" must not be detected.
[0021] 2. In a 5000ml beaker, add 1000ml of plasma, then add 2000ml of purified water for dilution, slowly add 750ml of a prepared saturated ammonium sulfate solution with a concentration of 20% while stirring;
[0022] 3. Use 10% hydrochloric acid to adjust the pH value to 5.70, put it in a 25°C water bath, and let it stand for 30 minutes;
[0023] 4. Centrifuge at 5000r / min for 10min and collect the supernatant;
[0024] 5. Add 500ml of 30% saturated ammonium sulfate solution to ...
Embodiment 2
[0030] Embodiment 2: a kind of method for extracting immunoglobulin G from pig blood, comprises the following steps:
[0031] 1. Collect healthy pig blood, and after anticoagulant treatment, the plasma is separated by a tubular separator. Healthy pig blood means that all pigs come from designated large-scale slaughterhouses, and must pass the inspection of the national animal quarantine department to be free of diseases, and additives such as melamine and "lean meat powder" must not be detected.
[0032] 2. Add 100L of plasma solution to the reaction tank, start stirring (the frequency of the frequency converter is 15Hz), then add 200L of purified water for dilution, after 5 minutes, slowly add 90L of saturated sulfuric acid solution with a concentration of 30%, and set the temperature at 25°C ;
[0033] 3. Use 10% HCl to adjust the pH value to 5.60. When the temperature is 25°C, stop stirring and let it stand for 35 minutes;
[0034] 4. Centrifuge at 14000-16000rpm for 10mi...
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Abstract
The invention provides a method for extracting immunoglobulin G from swine blood, comprising the following steps of: separating out blood plasma liquid from the swine blood subjected to anticoagulant treatment by using a tubular separator; mixing the blood plasma liquid and purified water in a proportion; uniformly stirring; adding a 20-30% saturated ammonium sulfate solution to carry out primary salt precipitation; adjusting a pH value to be 5.50-5.80; standing at constant temperature; centrifuging at high speed; adjusting the concentration of ammonium sulfate to be 30-40% by using supernate and then carrying out secondary salt precipitation; adjusting a pH value to be 7.20-7.60; standing at constant temperature; centrifuging at high speed to obtain an immunoglobulin G precipitate; dissolving the precipitate by using the purified water; and carrying out ultrafiltration and carrying out spray drying at low temperature to obtain an immunoglobulin G finished product. The extracting process is simple to operate, is capable of effectively shortening a production period, reducing the production cost and improving the product quality, and is suitable for industrial production, and the produced immunoglobulin G has the purity of 75% and the recycling rate of 88%..
Description
technical field [0001] The invention relates to a method for extracting immunoglobulin G from animal blood, in particular to a method for extracting immunoglobulin G from pig blood. Background technique [0002] Immunoglobulin (Immunoglobulin; Ig) has antibody activity or is similar to antibody in chemical structure, and is an important class of immune effector molecules. Proteins produced by lymphocytes of the immune system of higher animals can be converted into antibodies after being induced by antigens. Due to different structures, it can be divided into five types: IgA, IgM, IgD, IgE, and IgG. Most of them are gamma globulins, which are widely used in the field of biomedicine. [0003] IgG is the main Ig in biological fluids, accounting for about 70-75% of the total Ig in blood. Because IgG can cross the placenta, the Ig that newborns receive from their mothers plays an important role in fighting infection. Since IgG is more likely to diffuse into the extravascular s...
Claims
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