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Chlorhematin and preparation method thereof as well as blood-enriching pharmaceutical composition

A technology of hemin and its composition, which is applied in the field of hemin and its preparation method and blood-enriching drug composition, and can solve the problems of toxic side effects and poor absorption of ionized iron, etc.

Inactive Publication Date: 2011-05-11
SHANGHAI HONGRU SCI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to provide a new blood-enriching product, which has a high absorption rate and does not cause accumulation poisoning for existing blood-enriching products that supplement ionic iron. , good security

Method used

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  • Chlorhematin and preparation method thereof as well as blood-enriching pharmaceutical composition
  • Chlorhematin and preparation method thereof as well as blood-enriching pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Preparation of hemin

[0025] 1. Digestion: Mix anticoagulated fresh pig blood and glacial acetic acid containing 1 (wt)% NaCl in a volume ratio of 1:5 in a reaction kettle, and digest at 115° C. for 4 hours.

[0026] 2. Enzymolysis: Put the fluid obtained after digestion and 20 (wt)% acetic acid solution into the reaction kettle at a volume ratio of 1:6, start stirring at a speed of 60 rpm, and after stirring for 30 minutes, adjust the pH value of the reaction solution to 3.5. Then 537 protease (purchased from Jiangmen Yadong Biochemical Co., Ltd.) was added at a ratio of 0.5 (wt)% (equivalent to 500 IU / ml reaction liquid), and stirred at 60° C. for 48 hours. Then discharge.

[0027] 3. Filtration and separation: The reaction solution after enzymolysis is first filtered with a filter press, and then separated with a separator, and the rotation speed is required to be 7600 rpm. Take the solid particles after separation.

[0028] 4. Drying: Bake the soli...

Embodiment 2

[0029] Embodiment 2 Preparation of hemin

[0030] 1. Digestion: Mix anticoagulated fresh pig blood with glacial acetic acid containing 0.5 (wt)% NaCl in a volume ratio of 1:7 in a reactor, and digest at 100° C. for 8 hours.

[0031] 2. Enzymolysis: Put the fluid obtained after digestion and 30 (wt)% acetic acid solution into the reaction kettle at a volume ratio of 1:8, start stirring, and the rotation speed is required to be 60 rpm. After stirring for 30 minutes, adjust the pH value of the reaction solution to 4.5. Add 537 protease at a ratio of 0.1 (wt) %, and stir at 50° C. for 72 hours. Then discharge.

[0032] 3. Filtration and separation: The reaction solution after enzymolysis is first filtered with a filter press, and then separated with a separator, and the rotation speed is required to be 7600 rpm. Take the solid particles after separation.

[0033] 4. Drying: Bake the solid particles at 121°C for more than 2 hours to constant weight to obtain hemin. The purity ...

Embodiment 3

[0034] Embodiment 3 Preparation of hemin

[0035] 1. Digestion: Mix anticoagulated fresh pig blood with glacial acetic acid containing 2 (wt)% NaCl in a volume ratio of 1:3 in a reaction kettle, and digest at 130° C. for 1 hour.

[0036] 2. Enzymolysis: Put the fluid obtained after digestion and 10 (wt)% acetic acid solution into the reaction kettle at a volume ratio of 1:4, start stirring at a speed of 60 rpm, and after stirring for 30 minutes, adjust the pH value of the reaction solution to 4.0. Add 537 protease at a ratio of 1 (wt)% and stir at 70° C. for 36 hours. Then discharge.

[0037] 3. Filtration and separation: The reaction solution after enzymolysis is first filtered with a filter press, and then separated with a separator, and the rotation speed is required to be 7600 rpm. Take the solid particles after separation.

[0038] 4. Drying: Bake the solid particles at 121°C for more than 2 hours to constant weight to obtain hemin. The purity of hemin is 90%.

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Abstract

The invention discloses chlorhematin and a preparation method thereof as well as a blood-enriching pharmaceutical composition. The preparation method of the chlorhematin comprises the following steps: (1) mixing anticoagulant fresh porcine blood with glacial acetic acid containing 0.5-2wt% of NaCl, and digesting for 1-8 hours at the temperature of 100-130 DEG C; (2) uniformly mixing a fluid matter obtained after digestion in the step (1) with acetic acid solution, regulating the pH value to 3.5-4.5, and adding prolease for enzymolysis; and (3) filtering reaction solution after the enzymolysis in the step (2), and separating out solids, thus obtaining the chlorhematin. The biological extraction technology is adopted for preparing the chlorhematin, and the biological extraction technology can be used for getting the high-purity chlorhematin in a large-scale and pollution-free manner. The chlorhematin is a most effective raw material for treating iron deficiency and iron-deficiency anemia. According to the invention, the development of the new generation of high-efficient blood-enriching products is ensured to march into a new era of high-purity biological ion era with high safety and high absorption.

Description

technical field [0001] The invention belongs to the technical field of biochemical extraction, and particularly relates to a hemin, a preparation method thereof, and a blood-enriching medicinal composition. Background technique [0002] Iron deficiency and iron deficiency anemia are the most common nutrient deficiencies, and are still common and important health problems in countries all over the world, especially in developing countries, whose high-risk groups are women, infants and children; currently, about 1.12 billion people suffer from iron deficiency anemia, and 2.15 billion people are iron deficient. [0003] The status of iron deficiency and iron deficiency anemia in Shanghai according to the statistics of the affiliated hospitals of Fudan University is as follows: [0004] iron deficiency anemia iron deficiency women of childbearing age 11.39％ 43.32％ Women over three months pregnant 19.28％ 66.27％ Teenagers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18A61K31/555A61P7/06
Inventor 于茂泉官代学罗建华陈建吴海峰
Owner SHANGHAI HONGRU SCI TECH DEV
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