Safety evaluation method for pig plasma protein peptide

A technology of porcine plasma protein and plasma protein, which is applied in the field of food safety evaluation technology to evaluate the safety of porcine plasma protein peptides, and can solve problems such as unseen safety and toxicity research methods

Inactive Publication Date: 2010-12-15
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on bioactive peptides after enzymatic hydrolysis focuses on separation, purification and functional research, and there are no reports on related safety and toxicity research methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1 Materials and analysis methods

[0014] 1.1 Sample

[0015] The test sample is porcine plasma protein peptide powder.

[0016] 1.2 Test animals

[0017] Clean grade Kunming mice, 6-8 weeks old, weighing 18-20g.

[0018] 1.3 Statistical methods

[0019] Analysis of variance for measurement data and chi-square test for count data.

[0020] 2 Acute toxicity test in mice

[0021] 10 males and 10 males were pre-raised for 5 days to adapt to the environment. Take porcine plasma protein peptide powder, mix with water at a mass concentration of 0.5g / mL to form a paste. Before the administration of the test substance, the mice were fasted overnight for 16 hours, and 0.4mL / 20g was intragastrically administered once. The ambient temperature of the laboratory is 20-24°C, and the humidity is 55%-60%.

[0022] Continuously observe for 2 weeks, record the symptoms of mouse poisoning, pay attention to observe and record the time of occurrence of various symptoms, the apparent...

Embodiment 2

[0024] 1. Materials and analysis methods

[0025] 1.1 Sample

[0026] The test sample is pig plasma protein peptide powder;

[0027] The positive control substance is cyclophosphamide (prepared and used now);

[0028] The negative control was distilled water.

[0029] 1.2 Test animals

[0030] Kunming species (KM) mice.

[0031] 1.3 Statistical methods

[0032] Analysis of variance for measurement data; chi-square test for count data. A statistically significant difference was considered to be P<0.05.

[0033] 2. Genotoxicity experiment

[0034] 2.1 Bone marrow cell micronucleus test

[0035] Fifty Kunming (KM) mice aged 7-12 weeks were randomly divided into 5 groups, 10 in each group, half male and half male. They are divided into groups A, B, C, D, and E. Group A is the negative control group, group B is the high-dose group, group C is the middle-dose group, group D is the low-dose group, and group E is the cyclophosphamide positive control group. Group. The treat...

Embodiment 3

[0041] 1 Materials and analysis methods

[0042] 1.1 Sample

[0043] Pig plasma protein peptide powder.

[0044] 1.2 Test animals

[0045] Weaned SD rats, weighing 77-95g.

[0046] 1.3 Statistical methods

[0047] Analysis of variance for measurement data; chi-square test for count data. A statistically significant difference was considered to be P<0.05.

[0048] 2 30-day feeding trial

[0049] Get 80 rats, 40 males and 40 males, randomly divided into 4 groups, 3 test groups and a control group, 10 females and 10 males and females in each group, weighed before the test, the difference in animal weight is not more than 120% of the average body weight. The preparation method of the test substance in the middle-dose group and the low-dose group is as follows: take plasma protein peptide powder and full-price feed pigs, mix them uniformly in the ratio of 1:1 and 1:4 respectively, and then use a granulator to compress the powder containing protein peptide. The powder mass fr...

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Abstract

The invention provides a safety evaluation method for plasma protein peptide. According to the method, national seriously-wasted pig blood is selected as a raw material for extracting plasma protein, and pig plasma bioactive peptide is prepared by enzymolysis. Experimental study on acute toxicity and genetic toxicity in a mouse is performed for evaluating the safety of the pig plasma bioactive peptide and providing scientific proof for development and reasonable and safe use of animal blood polypeptide health-care food.

Description

technical field [0001] The invention belongs to the field of food safety and uses food safety evaluation technology to evaluate the safety of pig plasma protein peptides. Background technique [0002] Active polypeptides not only have nutritional and biological activities, but also have the characteristics of rapid absorption, so the application prospects of active polypeptides in food are broad. At present, domestic research on bioactive peptides after enzymatic hydrolysis focuses on separation, purification and functional research, and there are no reports on related safety and toxicity research methods. However, in order to ensure the food safety of peptides in food research and development, relevant safety and toxicology research is also essential. In order to evaluate its safety, we conducted acute toxicity, genotoxicity experiments and 30-day feeding experiments to provide scientific basis for the development of animal blood polypeptide health food and its rational an...

Claims

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Application Information

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IPC IPC(8): G01N33/02
Inventor 潘风光吴涵李洪山赵娅娅宫新统
Owner JILIN UNIV
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