Preparation method of porcine thrombin
A technology for porcine thrombin and prothrombin, which is applied in the field of thrombin preparation, can solve the problems of low yield of porcine thrombin, unsuitable for large-scale production, etc., and achieves improved biological safety, convenient large-scale production, and high thrombin harvest. rate effect
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specific Embodiment approach 1
[0019] Specific embodiment one: the preparation method of a kind of porcine thrombin of the present embodiment is carried out according to the following steps:
[0020] 1. Separation of pig plasma: take fresh pig blood, add anticoagulant, and centrifuge to obtain pig plasma; wherein, the mass ratio of fresh pig blood to anticoagulant is 250:1;
[0021] 2. Chemical virus inactivation: add virus inactivation reagent according to 10% of the total weight of pig plasma, and incubate at 24-26°C for 6-8 hours to inactivate virus;
[0022] 3. Adsorption of prothrombin: add ion exchange resin according to 1.5% of the total weight of porcine plasma, adsorb at room temperature for 40-60 minutes, then filter and collect ion exchange resin with filter cloth, and use 5-10 times the washing liquid of ion exchange resin weight , washing the ion exchange resin, discarding the washing liquid;
[0023] 4. Collect prothrombin: the ion exchange resin washed with the washing solution in step 3 is ...
specific Embodiment approach 2
[0028] Embodiment 2: This embodiment differs from Embodiment 1 in that the anticoagulant described in step 1 is trisodium citrate with a mass percentage of 0.4%. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0029] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the virus inactivation reagent described in step two comprises the aqueous solution of polysorbate-80 and tributyl phosphate, wherein polysorbate methyl Ester-80 is 11% by volume, tributyl phosphate is 3.3% by volume, and the rest is water. Others are the same as in the first or second embodiment.
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