An edible and medicinal fungal polysaccharide low-temperature normal-pressure plasma extraction method includes the first step of adsorption resin preprocessing, the second step of fungal polysaccharide primary extraction, the third step of low-temperature normal-pressure plasma extraction, the fourth step of porous adsorption resin extraction, the fifth step of alcohol precipitation and the sixth step of drying. The fungal polysaccharide prepared through extraction is prepared from lentinula edodes, hericium erinaceus, ganoderma lucidum, black fungi, grifola frondosa, white fungi, flammulina velutipes and any one or combination of edible and medicinal fungi containing polysaccharide compounds. According to the edible and medicinal fungal polysaccharide low-temperature normal-pressure plasma extraction method, the extraction period is shortened, the use amount of solvents is small, production efficiency is high, energy is saved, consumption is reduced, and extraction purity is high.