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Bilberry extract and preparation method thereof

An extract, English technology, applied in the field of European bilberry extract and the preparation of this extract, can solve problems such as difficult methods, and achieve the effect of easy operation

Inactive Publication Date: 2016-02-03
TIANJIN NANKAI SHARE PHARMA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it lacks the material basis for providing high-concentration anthocyanins for many studies. It is still so difficult to obtain high-concentration total anthocyanins, and it is even more difficult to separate high-concentration monomers from it. Therefore, a high-concentration anthocyanin is urgently needed. Total anthocyanins are used as the material basis for pharmacological, clinical, and further separation and purification of monomers; of course, in order to obtain such high-concentration anthocyanins, it is first necessary to establish a simple and efficient anthocyanin extract preparation method and detection method as technical support

Method used

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  • Bilberry extract and preparation method thereof
  • Bilberry extract and preparation method thereof
  • Bilberry extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The acidic aqueous solution containing bilberry total anthocyanins is prepared by one of the following methods.

[0075] Method A: Crack the lingonberry fruit into pulp with a tissue homogenizer. The volume fraction of 2-5 times the pulp mass is 30-80%, and the pH value is adjusted to acidic ethanol aqueous solution with hydrochloric acid, heated and refluxed at 40-60°C for 2-3 times, filtered, the filtrates are combined, the ethanol is recovered, concentrated .

[0076] Method B: Weighing a commercially available bilberry extract with a total anthocyanin content of 36% to prepare an aqueous solution.

Embodiment 2

[0078] The macroporous adsorption resin used is prepared by the following method:

[0079] Prepare an aqueous solution with a mass concentration of polyvinyl alcohol of 0.5-2% and a sodium chloride mass concentration of 5-10%, which is the aqueous phase of suspension polymerization; mix divinylbenzene (DVB) with a mass percentage of 50-80% and ester mono The body is mixed according to the feeding ratio, and the porogen and initiator are added, and after mixing evenly, it becomes the suspension polymerization oil phase. Wherein, the ester monomer is one or more of ethylene glycol dimethacrylate, methyl acrylate and methyl methacrylate. The feeding ratio of DVB and ester monomer is 19:1-7:3. The porogen can be one or more of toluene, 200# gasoline, cyclohexane, and methylcyclohexane, and the amount added is 1-3 times the total mass of DVB and ester monomers. The initiator is azobisisobutyronitrile or benzoyl peroxide, and the addition amount is 1-2% of the mass sum of DVB and ...

Embodiment 3

[0084] The macroporous adsorption resin prepared by the above method is fully swollen with ethanol, loaded into a resin column, washed with ethanol until the effluent is mixed with water in equal volumes and is not cloudy, and then washed with water until the effluent has no alcohol smell, and the macroporous adsorption resin column is obtained.

[0085] The acid aqueous solution is passed into the macroporous adsorption resin column obtained by the above method for adsorption. After the adsorption is completed, wash with acidic water until the effluent is clear, then use acidic ethanol or methanol or acetone aqueous solution as a desorbent for desorption, and receive the eluent in sections, preferably with a pH value between 2-5, and combine the total anthocyanins The part whose content is 55%-95%. Concentrate, spray dry, the air inlet temperature is 130-140°C, and the outlet air temperature is 60-65°C to obtain the finished product. The obtained results are listed in Table ...

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Abstract

The invention relates to a plant extract, in particular to a bilberry extract and a method for preparing the extract. The content of the bilberry extract is 55%-95%, and the bilberry extract is consistent to finger-print specified in European Pharmacopoeia. The invention also relates to the method for preparing the extract. The method comprises the following steps of extracting the bilberry extract by using macroporous adsorption resin; and concentrating and performing spraying drying on collected extracting solution. According to the method, rate of charge of divinylbenzene to esters monomers of the macroporous adsorption resin used in the method is 19:1-7:3, and a desorption agent used in a desorption process is ethanol or an acid aqueous solution of methyl alcohol or acetone.

Description

technical field [0001] The invention relates to a plant extract, in particular to a bilberry extract and a method for preparing the extract. Background technique [0002] BV: resin column volume [0003] DVB: divinylbenzene [0004] Total anthocyanins: It is a mixture of 15 kinds of anthocyanins, which are: [0005] 1. Delphinidin-3-O-galactoside chloride, English name delphinidin-3-O-galactosidechloride [0006] 2. Delphinidin-3-O-glucoside chloride, English name delphinidin--3-O-glucosidechloride [0007] 3. Chlorinated cyanidin-3-O-galactoside, English name cyanidin-3-O-galactodisechloride [0008] 4. Delphinidin-3-O-arabinoside chloride, English name delphinidin-3-O-arabinosidechloride [0009] 5. Chlorinated cyanidin-3-O-glucoside, English name cyanidin-3-O-glucosidechloride [0010] 6. Petunidin-3-O-galactoside chloride, English name petunidin-3-O-galactosidechloride [0011] 7. Chlorinated cyanidin-3-O-arabinoside, English name cyanidin-3-O-arabinosidechloride ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45A61K31/7048A23L1/30
Inventor 姚丽娟皮国沛黄牧童杨秀伟闫志刚
Owner TIANJIN NANKAI SHARE PHARMA SCI & TECH
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