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Technique method of extracting heparin sodium by virtue of macroporous polymeric adsorbent

A process method and pore adsorption technology are applied in the field of macroporous adsorption resin to extract heparin sodium, which can solve problems such as affecting the quality of final products.

Inactive Publication Date: 2013-03-06
JIANGSU LIANZHONG CASING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional refining process of heparin sodium includes oxidation, alcohol precipitation, and drying to obtain high-quality products, but this method cannot completely remove the residual impurities in crude heparin sodium, which affects the quality of the final product

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0010] A process for extracting heparin sodium with a macroporous adsorption resin, the steps are as follows:

[0011] Dissolve 100g of heparin sodium crude product in 1000ml of pure water, add 3wt% trypsin for 2-3h enzymolysis, after enzymolysis, adjust the pH value of the heparin sodium solution to 2.5 with hydrochloric acid, and use Amberlite XAD16 macroporous adsorption resin separation column to adsorb, The flow rate is 3BV / h, the column temperature is 20°C, after the adsorption is saturated, the resin bed is washed with 2BV of clear water at a rate of 2BV / h, and then, the separation column with saturated adsorption is eluted with 1mol / l sodium hydroxide. Desorption at room temperature, the elution flow rate is 2BV / h, the amount of eluent is 4BV, separated to obtain heparin sodium solution, and then undergoes conventional oxidation, alcohol precipitation, and drying to obtain heparin sodium refined product with a potency of 162.1U / mg.

Embodiment 2

[0013] A process for extracting heparin sodium with a macroporous adsorption resin, the steps are as follows:

[0014] Dissolve 100g of heparin sodium crude product in 1000ml of pure water, add 3wt% trypsin for 2-3h enzymolysis, after enzymolysis, adjust the pH value of heparin sodium solution to 2 with hydrochloric acid, and use Amberlite XAD16 macroporous adsorption resin separation column to adsorb, The flow rate is 2BV / h, the column temperature is 40°C, after the adsorption is saturated, the resin bed is washed with 2BV of water at a rate of 1BV / h, and then, the separation column with saturated adsorption is eluted with 1mol / l sodium hydroxide. Desorption at room temperature, the elution flow rate is 1BV / h, the amount of eluent is 2BV, separated to obtain heparin sodium solution, and then undergoes conventional oxidation, alcohol precipitation, and drying to obtain heparin sodium refined product with a potency of 161.1U / mg.

Embodiment 3

[0016] A process for extracting heparin sodium with a macroporous adsorption resin, the steps are as follows:

[0017] Dissolve 100g of heparin sodium crude product in 1000ml of pure water, add 3wt% trypsin for 2-3h enzymolysis, after enzymolysis, adjust the pH value of heparin sodium solution to 2 with hydrochloric acid, and use Amberlite XAD16 macroporous adsorption resin separation column to adsorb, The flow rate is 2BV / h, the column temperature is 20°C, after the adsorption is saturated, the resin bed is washed with 2BV of clean water at a rate of 1BV / h, and then the saturated separation column is eluted with 1mol / l sodium hydroxide. Desorption at room temperature, the elution flow rate is 1BV / h, the amount of eluent is 2BV, separated to obtain heparin sodium solution, and then undergoes conventional oxidation, alcohol precipitation, and drying to obtain heparin sodium refined product with a potency of 163.4U / mg.

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Abstract

The invention discloses a technique method of extracting heparin sodium by virtue of a macroporous polymeric adsorbent, wherein the technique method comprises: adding an adsorption step in the traditional refining procedure, utilizing AmberliteXAD16 polymeric adsorbent using polystyrene as a base material to absorb a rough product heparin sodium acid solution, desorbing by use of a sodium hydroxide eluent, and finally, performing alcohol precipitation and drying treatment to obtain heparin sodium with titer larger than 160 U / mg, wherein residual heteropolymeric protein in the rough product can be completely removed.

Description

technical field [0001] The invention relates to a process method for extracting heparin sodium with a macroporous adsorption resin. Background technique [0002] Heparin sodium is a kind of anticoagulant drug, which is mainly the sodium salt of dextran sulfate sulfate extracted from the intestinal mucosa of pigs or cattle, and belongs to mucopolysaccharides. Heparin sodium can be used in surgery to prevent dangerous thromboembolic diseases, can relieve unstable angina, prevent myocardial infarction, and reduce the mortality rate of acute myocardial infarction. The latest research has found that it also has the effect of lowering blood fat and cholesterol. [0003] The traditional refining process of heparin sodium includes oxidation, alcohol precipitation, and drying to obtain high-quality products, but this method cannot completely remove the residual impurities in crude heparin sodium, which affects the quality of the final product. Contents of the invention [0004] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 顾永军
Owner JIANGSU LIANZHONG CASING
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