Process for synthetic extraction of polysaccharides, tea-polyphenol, theanine, caffeine from tea

A technology of comprehensive extraction and tea extraction, which is applied in the field of caffeine, comprehensive extraction of tea polysaccharides, tea polyphenols, and theanine, can solve the problems of reducing the economic benefits of tea deep processing, extraction and separation of theanine, and the inability to make full use of it. The effect of less loss of active substances, short extraction time, and high content of tea polysaccharides

Inactive Publication Date: 2004-12-29
TEA RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, this method only develops and utilizes tea polysaccharide, a component of tea, and cannot make full use of other active ingredients in tea, such as tea polyphenols, theanine, caffeine, etc., resulting in a waste of resources and reducing the economic benefits of deep processing of tea.
[0006] At present, there are ...

Method used

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  • Process for synthetic extraction of polysaccharides, tea-polyphenol, theanine, caffeine from tea

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) 1 kg of tea powder, under the action of microwave, use 5 times of ethanol aqueous solution, soak and extract repeatedly at 60 ° C for 4 times, each time for 5 minutes, and separate by filtration to obtain the tea extract, which is concentrated and concentrated. Edible ethanol was added to the mixture, and the dosage was twice the volume of the concentrated solution. After filtration, the tea polysaccharide precipitate and the residual liquid after polysaccharide extraction were obtained. After the polysaccharide precipitate was dissolved in water, 1% zinc acetate was added to remove the protein, and the polysaccharide solution was subjected to gel G75 column chromatography. , the eluate was dried to obtain tea polysaccharide, the total sugar content in the tea polysaccharide reached 65.00%, and the yield was 0.5%.

[0030] 2) After polysaccharide extraction, the residual liquid is extracted with chloroform, the amount of chloroform is 1 times the volume of the liquid ...

Embodiment 2

[0034] 1) 1 kg of tea powder, under microwave action, use 10 times of 30% ethanol aqueous solution, soak and extract repeatedly at 40°C for 3 times, each time for 20 minutes, filter and separate to obtain tea extract, the tea extract is concentrated, Add edible ethanol to the concentrated solution, the dosage is 3 times the volume of the concentrated solution, filter to obtain the tea polysaccharide precipitate and the residual liquid after polysaccharide extraction, dissolve the polysaccharide precipitate in water, add 1% zinc acetate to remove protein, and pass the polysaccharide solution through the gel G25 column Chromatography, drying the eluent to obtain tea polysaccharide, the total sugar content in the tea polysaccharide reaches 66.10%, and the yield is 0.51%.

[0035]2) After the polysaccharide is extracted, the residual liquid is extracted with chloroform, the amount of chloroform is 5 times the volume of the liquid phase, mixed evenly, and the two phases are separate...

Embodiment 3

[0039] 1) 1 kg of tea powder, under microwave action, use 15 times of 10% ethanol aqueous solution, soak and extract repeatedly at 40°C for 3 times, each time for 15 minutes, obtain tea extract through filtration and separation, and concentrate the tea extract. Edible ethanol is added to the concentrated solution, the dosage is 5 times the volume of the concentrated solution, and the tea polysaccharide precipitate and the residual liquid after polysaccharide extraction are obtained by filtration. After the polysaccharide precipitate is dissolved in water, 1% zinc acetate is added to remove the protein, and the polysaccharide solution is passed through the gel Bio- Gel P-6 column chromatography, the eluate was dried to obtain tea polysaccharide, the total sugar content in the tea polysaccharide reached 67%, and the yield was 0.55%.

[0040] 2) After the polysaccharide is extracted, the residual liquid is extracted with chloroform, the amount of chloroform is 3 times the volume o...

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Abstract

The comprehensive process of extracting tea polysaccharide, tea polyphenol, theanine and coffine from tea includes the following steps: water or organic solvent extraction of tea powder under the action of microwave, concentration, precipitation and water dissolving the extract liquid, salt separating to eliminate protein and separation in gel column and drying to obtain tea polysaccharide; chloroform extraction of the raffinate, ethyl acetate extraction of the chloroform raffinate and drying to obtain tea polyphenol; No. 732 cationic exchange resin extraction of the ethyl acetate raffinate, concentration, crystallization and drying to obtain theanine; and crystallizing and drying the chloroform extract liquid to obtain coffine. The present invention has the advantages of short extraction time, high extraction rate, less loss of active matter, etc.

Description

technical field [0001] The invention relates to a method for comprehensively extracting tea polysaccharides, tea polyphenols, theanine and caffeine from tea leaves. Background technique [0002] Tea is one of my country's important economic crops and export products that earn foreign exchange. In 2000, foreign exchange earnings reached 400 million U.S. dollars. However, for a long time, my country's tea production is still dominated by traditional rough-processed products. The backlog of low-grade tea left and right has seriously hindered the development of my country's tea industry. [0003] Tea deep processing is a new field of tea industry, which has huge value-added potential and market potential. With the continuous deepening of tea research and the intersection and penetration of medical research, it has been confirmed that tea polysaccharides have health functions such as lowering blood sugar, enhancing immunity, lowering blood lipids, anti-...

Claims

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Application Information

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IPC IPC(8): B01D11/02C07C237/06C07D311/62C07D473/12C08B37/00
Inventor 江和源王川丕
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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