Method for detecting difloxacin mesylate in pig plasma and alveolar fluid

A technology of danfloxacin mesylate and pig plasma, which can be used in measurement devices, instruments, scientific instruments and other directions, can solve problems such as affecting drug detection, and achieve the effects of high sensitivity, good stability and good repeatability

Inactive Publication Date: 2019-09-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Animal blood samples contain more impurities, which affect the detection of drugs

Method used

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  • Method for detecting difloxacin mesylate in pig plasma and alveolar fluid
  • Method for detecting difloxacin mesylate in pig plasma and alveolar fluid
  • Method for detecting difloxacin mesylate in pig plasma and alveolar fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The detection of danofloxacin in the pig plasma of embodiment 1

[0031] (1) Sample pretreatment of pig plasma

[0032] Put 5 mL of the collected fresh pig blood sample into a plastic centrifuge tube containing 0.5 mL of sodium heparin with a mass fraction of 1%, centrifuge at 3500 r / min for 10 min, and separate the layers. The upper layer is plasma.

[0033] Sample pretreatment: draw 0.5mL blank plasma into a 10mL EP tube, add 2mL acetonitrile to extract, vortex for 5min, centrifuge at 10000r / min for 10min, take the supernatant, add 2mL acetonitrile to the precipitate for 2 extractions, centrifuge at 10000r / min for 10min, The supernatants from the two extractions were combined, dried under nitrogen at 50°C, the residue was dissolved in 0.5 mL of the initial mobile phase, centrifuged at 10,000 r / min for 10 min, the supernatant was taken, passed through a 0.22 μm filter membrane, and loaded. The initial mobile phase is a mixture of phosphoric acid and acetonitrile, the ...

Embodiment 2

[0049] The detection of danofloxacin in the porcine alveolar fluid of embodiment 2

[0050] (1) Pretreatment of porcine alveolar fluid samples

[0051] The collected fresh porcine alveolar lavage fluid was centrifuged at 800r / min for 10min, and the supernatant was separated to obtain porcine alveolar fluid.

[0052] Sample pretreatment: draw 0.5mL blank alveolar fluid into a 10mL EP tube, add 2mL acetonitrile for extraction, vortex for 5min, centrifuge at 10000r / min for 10min, take the supernatant, add 2mL acetonitrile to the precipitate for 2 extractions, centrifuge for 10min at 10000r / min , combined the supernatants of the two extractions, blown dry with nitrogen at 50°C, dissolved the residue with 0.5mL of the initial mobile phase, centrifuged at 10000r / min for 10min, passed through a 0.22μm filter membrane, and loaded the sample.

[0053] (2) HPLC detection of porcine alveolar fluid samples

[0054] Chromatographic conditions: Chromatographic column: Agilent SB-Aq, 250×4...

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Abstract

The invention discloses a method for detecting difloxacin mesylate in pig plasma and alveolar fluid. The method includes the following steps that (1) an objective compound in the pig plasma and the alveolar fluid is extracted by adopting acetonitrile, and supernatant is obtained after centrifugation; (2) blowing is conducted with nitrogen at 50 DEG C until drying, redissolution, centrifugation, and filtration are conducted, and sample extractive is obtained; and (3) a sample is fed, high performance liquid chromatography detection is conducted, a mobile phase is acetonitrile-0.05% phosphoric acid, a phase A is the acetonitrile, a phase B is the 0. 05% phosphoric acid, and gradient elution is conducted. The method for extracting the sample and detection the difloxacin mesylate in the pig plasma and the alveolar fluid is established, and the method has the characteristics of being simple, fast and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of clinical detection of veterinary drug metabolism, in particular to a method for detecting dafloxacin mesylate in pig plasma and pig alveoli. Background technique [0002] Danofloxacin (Danofloxacin) is the third generation of animal-specific fluoroquinolones. It can inhibit the synthesis of bacterial DNA gyrase and exhibit a bactericidal effect. It has a broad antibacterial spectrum, strong bactericidal activity, wide distribution in the body, and no crossover with other antibacterial drugs. characteristics of drug resistance. After administration in the form of mesylate, it has rapid absorption, high bioavailability and high concentration in tissues. At present, danofloxacin has been approved in many countries in Asia, Europe, North America and Latin America for the treatment of respiratory diseases caused by bacterial and mycoplasma infections in animals such as cattle, pigs and chickens. Therefore, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/14G01N30/02
CPCG01N30/02G01N30/14
Inventor 郝海红袁宗辉徐紫慧罗讯黄玲利王玉莲彭大鹏王旭陈冬梅陶燕飞潘源虎谢书宇程古月瞿玮刘振利谢长清房诗薇黄啸
Owner HUAZHONG AGRI UNIV
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