74 results about "Population screening" patented technology

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.

A sensor technology comprising a single nano-material (gold nanoparticles and/or carbon nanotube) based sensor or a plurality of sensors in conjunction with a pattern recognition algorithm for non-invasive and accurate diagnosis of tuberculosis caused by M. tuberculosis bacteria in a subject. The sensor technology is suitable for population screening of tuberculosis, particularly in resource-poor and developing countries.

The present invention discloses a resource scheduling method for a cloud system. The method comprises the following steps of: in a population initialization step, an alternative scheme of resource scheduling of the cloud system is obtained, and initial population is created by utilizing the alternative scheme; in a fitness function creation step, a total fitness function is created for specific demands of resource scheduling of the cloud system; in a population screening step, individuals in the initial population are screened by utilizing the total fitness function to obtain fitted individuals; and in a resource scheduling step, a virtual machine and a node controller are subjected to resource scheduling according to the alternative scheme corresponding to the fitted individuals. Compared to the prior art, the resource scheduling method disclosed by the present invention is relatively low in transfer cost while realizing good load balancing result.

The invention discloses a method for constructing a serum metabonomics analysis model. The construction method comprises the following steps: collecting a healthy serum sample and an ill serum sample; performing LC-MS detection on the samples, thereby obtaining original metabolic fingerprints; preprocessing the fingerprints, sequentially performing principal component analysis and partial least square discriminant analysis on a two-dimensional matrix, thereby obtaining a PLS-DA model; and verifying the obtained PLS-DA model, wherein if the overfitting risk does not exist, the model construction is finished. According to the method disclosed by the invention, the serum metabonomics analysis technology is applied to early screening of an esophagus cancer, the high-risk population of the esophagus cancer can be rapidly and conveniently screened by virtue of model construction, the esophagus cancer screening range is widened, the entire population screening efficiency in the high incidence area of esophagus cancer is effectively improved, the screening cost is greatly reduced, pain of partial population caused by an invasive gastroscope is effectively avoided, and the method has significant economic and social benefits and is worthy of popularization and application.

The invention relates to a spinal muscular atrophy pathogenic gene detection kit based ona melting curve analysis, and relates to a pathogenic gene detection kit, which comprises an upstream primer F1 and a downstream primer R1 for amplifyingexon7 of SMN1 and SMN2 genes; an upstream primer F2 and a downstream primer R2 for amplifying exon4 of an internal reference CFTR gene; and a fluorescent probe for detection. In a single-tube PCR system, the copy number of exon7 of the SMN1 gene as a main pathogenic gene of SMA can be quantitatively detected, the sample genotype can be known by a fluorescence PCR melting curve analysis after PCR amplification is finished, the whole operation is completed in 2 to 3h, is simple and rapid, and is short in time-consuming; the homogeneous detection and closed tube operation are carried out; the detection flux is high; the detection specificity is high, and the results are easy to interpret. The detection kit can be rapidly and convenientlyapplied to large-scale population screening of the SMA pathogenicgene, and is especially suitable for prenatal, premaritaland pre-pregnancy screening and genetic diagnosis of patients.

The invention belongs to the field of biological immunochromatography detection methods and specifically relates to a nano selenium kit for quickly detecting HE4 and CA125 and a preparation method thereof. The kit is composed of a sample pad, a combined pad, a reaction pad, a water absorption pad and a PVC bottom plate, wherein a detection line T1 coated by an anti-CA125 antibody A2, a detection line T2 coated by an anti-HE4 antibody B2 and a quality control line C coated by goat anti-rat IgG are arranged on the reaction pad; by means of observing color development reaction of the detection lines, bedside quick screening diagnosis of early ovarian cancer can be achieved. The prepared nano selenium immunochromatography kit disclosed by the invention has the advantages of strong specificity,high sensitivity, good accuracy, convenience, quickness, small sample amount to be detected and suitability for screening, homelab and bedside quick detection of high-risk groups for ovarian cancer.

The invention discloses a rapid detection kit for a novel coronavirus antigen. The rapid detection kit comprises a kit body and a reagent strip placed in the kit body. The reagent strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption padare sequentially overlapped end to end along the length direction of the bottom plate. The combination pad is coated with an anti-nucleocapsid protein monoclonal antibody and an anti-spinous glycoprotein monoclonal antibody which are labeled by colloidal gold; and the nitrocellulose membrane is coated with a nucleocapsid protein detection line N line formed by an anti-nucleocapsid protein monoclonal antibody, a spinous glycoprotein detection line S line formed by an anti-spinous glycoprotein monoclonal antibody, and a quality control line C line formed by a goat anti-mouse IgG antibody. The invention also discloses a preparation method and an application of the kit. The rapid detection kit prepared in the invention can be used for directly detecting the novel coronavirus, is simple and convenient to operate and short in detection time, and is suitable for large-scale population screening and on-site screening.

The invention relates to the technical field of microorganisms, in particular to a new corona-virus nucleic acid 10-in-1 test (10-in-1 test) technology. The new corona-virus nucleic acid 10-in-1 testtechnology is a method for collecting 10 swabs collected from 10 persons in one collection tube and detecting, and the used materials comprise a virus collection tube and a collection swab. The 10-in-1 test technology provided by the invention is suitable for large-area general screening of people with low risk of new corona-virus infection, and can significantly reduce medical collection consumables required by large-scale population screening and reduce the nucleic acid detection cost of new corona-virus; according to the method, the low nucleic acid detection efficiency of a traditional collection and detection method is avoided, the SARS-CoV-2 infection can be effectively diagnosed as soon as possible, further diffusion of the SARS-CoV-2 infection can be controlled, and epidemic spreading can be prevented in time.

The present invention provides a primer set for detecting alpha thalassemia and beta thalassemia, and belongs to the field of molecular biology, wherein the primer set comprises an alpha primer set and a beta primer set. The invention further provides a chip capable of simultaneously detecting alpha thalassemia and beta thalassemia, wherein probes having sequences represented by SEQ ID NO:1-31 areimmobilized on the chip. Based on the primer set and the chip, the present invention further provides a kit capable of simultaneously detecting alpha thalassemia and beta thalassemia. The kit of theinvention has advantages of short detection time, low cost, convenient operation, closed tube operation and pollution reducing, and has great significance in the screening of thalassemia population, the genetic counseling and the prenatal diagnosis.

The invention relates to a quantitative fluorescent multiplex PCR test kit for Epstein-Barr virus, and the kit comprises the following reagents: a, specific primer pairs for amplifying mutations of key target genes in the Epstein-Barr virus; and b, specific probes for the mutations of key target genes in the Epstein-Barr virus, wherein the mutations are P-thr, V-val and V-leu in the coding region of EBNA-1 gene, promoters Cp, Fp and Zp of the EBNA-1 gene, and XhoI-loss, 30bp Deletion and Ser 366 Thr of LMP1 gene. The test kit is advantageous in that: the aim of precise prediction of the risk of nasopharyngeal carcinoma in high risk population can be achieved; the main carcinogenic mutations can be well known for the combination of population screening and population intervention for nasopharyngeal carcinoma; and the kit has the advantages of high in sensitivity, fast in test, and low in cost, having broad market prospect.

The invention is a quantitative detecting technique of trace circular DNA based on fluorescent dye. It analyzes the strength of the fluorescence generated by combining the dye with purified DNA to determine the content of dissociative DNA in peripheral blood. It can accurately detect trace circular DNA down to a level of nano g, and has the characters of rapidness, simplicity and convenience, sensitivity, and accuracy. The determination of special people and tumor patient verifies that it has higher reliability, sensitivity and accuracy. It can be used in screening large-scale high-danger tumor people, early tumor diagnosis and dynamic observation of curative effect prognosis.

The invention discloses a typing detection kit for Candida albicans. The typing detection kit comprises a gene chip and various primers. The gene chip is provided with 13 different types of nucleotide sequence probes for the Candida albicans, a DNA sequence marked with biotin points and a DNA sequence with a coding beta-globulin part, wherein the probes are in the sequences of SEQ ID Nos: 1-13 or sequences complementary with the SEQ ID Nos: 1-13, the DNA sequence with the biotin points is SEQ ID No. 14, and the DNA sequence with the coding beta-globulin part is SEQ ID No. 15. The various primers are in the sequences of SEQ ID Nos: 16-21. By means of the typing detection kit for the Candida albicans, a typing joint detection platform for detecting the Candida albicans is provided, synchronous joint detection can be achieved, the detection of specificity can be enhanced, the cost can be lowered, the detection time can be shortened, and the typing detection kit has great significance for carrying out clinic early diagnosis and population screening for the Candida albicans.

The invention discloses Citrin immunodeficiency disease pathogenic gene SLC25A13 high-frequency I-type mutation screening primers and kit, belongs to the technical field of biology. Four newly designed primers are provided; a multi-PCR system depending on an ordinary rTaq enzyme is built; whether a sample is I mutation positive or negative or heterozygote can be identified through once PCR and analysis of an electrophoretogram; the primers and the kit are accurate, simple, fast and low in cost. By the screening primers and kit disclosed by the invention, DNA sequencing is not needed; the screening primers and kit are simple, fast and low in cost; the detection result is direct and reliable; the detection time and cost are superior to those of an existing method; the screening primers and kit can be finished by simple common equipment and are suitable for medical and test mechanisms of various regions for fast detection and large-scale population screening of the SLC25A13 high-frequency I-type mutation.

The invention discloses molecular markers for identifying the PHYB wild type and mutant of a rice phytochrome gene. The nucleotide sequences of the markers related to phyB wild type site detection and phyB mutant site detection are shown as Seq No.1-2 respectively. A primer PHYBF4/PHYBR1 is used for carrying out PCR amplification, and an amplification product is subjected to BamH I digestion and electrophoretic analysis, wherein if the PCR product can not be digested by BamH I and is a 175bp DNA fragment, the PCR product is the wild type phyB, and if the PCR product can be digested by BamH I, and the digestion products are a 25bp DNA fragment and a 151bp DNA fragment, the PCR product is the mutant phyB1. The molecular markers have the advantages that amplification is easy and fast, and cost is low. The molecular markers can be used for effectively, rapidly and reliably identifying the phyB allelotype, and a powerful tool is provided for subsequent application of the phyB mutant in breeding population screening.

The invention is applicable to the fields of biotechnology and ophthalmic health, and provides a molecular marker of a myopia-related susceptibility gene as well as a detection primer group and application of the molecular marker. The detection primer group of the molecular marker comprises multiple PCR amplification upstream and downstream primer pairs of 37 SNP loci of 24 high myopia and pathological myopia-related susceptibility genes of human genome DNA, and a single base extension primer. According to the invention, sample nucleic acid mass spectrum detection can be carried out by using amultiplex PCR amplification technology, the 37 SNP loci can be detected at one time by double reaction holes, through correlation analysis of highly myopia susceptibility genes and genetic risks, a highly myopia genetic risk level assessment method special for Chinese population is provided by using a highly myopia genetic risk assessment system, and the method has the advantages of high detection flux, high efficiency, high accuracy, low cost and the like, and is suitable for large-scale population screening.