Joint detection kit for alpha,beta-thalassemia associated mutant genes

Abstract

The invention discloses a joint detection kit for detecting alpha,beta-thalassemia associated with mutant genes, which comprises (1) a gene chip and (2) a primer, wherein the gene chip is provided with probes, and the probes are a sequence SEQ ID Nos:1-31 and a sequence which is complementary to the sequence SEQ ID Nos:1-31; and the primer is a sequence SEQ ID Nos:32-42. The thalassemia gene detection kit provides a platform for detecting 16 mutant genes associated with alpha-thalassemia (three deletion types and two mutant types) and beta-thalassemia, can perform synchronous joint detection,improve the specificity of detection, reduce cost and shorten detection time, and has a great significance for the screening of patients suffering from thalassemia, genetic counseling and prenatal diagnosis.

Description

technical field

[0001] The invention relates to the field of genes, in particular to a combined detection kit for α, β-thalassemia mutation genes. Background technique

[0002] Thalassemia (hereinafter referred to as thalassemia) is a hemolytic anemia caused by an imbalance in the synthesis rate of α, β-globin peptide chains due to human gene mutation or deletion. Thalassemia is one of the most common genetic diseases in the world, and it is widely distributed in Mediterranean countries and other areas where malaria used to be high. In my country, the provinces south of the Yangtze River have a high incidence rate. In addition to the highest incidence rates in Guangdong, Guangxi, and Hainan provinces, Guizhou, Yunnan, and Sichuan are also high-incidence areas. The two common types of thalassemia are alpha-thalassemia and beta-thalassemia.

[0003] α-thalassemia is a chronic hemolytic disease caused by autosomal genetic defect, which causes the loss or dysfunction of α-glob...

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