Process of micro circulating DNA by quantitative testing

A quantitative detection method and quantitative detection technology, applied in the field of medical testing, can solve problems such as complicated operations, restrictions on the development of similar research, unacceptable prices, etc., and achieve the effect of cheap, universal and convenient use
CN1560277AInactive Publication Date: 2005-01-05FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2005-01-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention is a quantitative detecting technique of trace circular DNA based on fluorescent dye. It analyzes the strength of the fluorescence generated by combining the dye with purified DNA to determine the content of dissociative DNA in peripheral blood. It can accurately detect trace circular DNA down to a level of nano g, and has the characters of rapidness, simplicity and convenience, sensitivity, and accuracy. The determination of special people and tumor patient verifies that it has higher reliability, sensitivity and accuracy. It can be used in screening large-scale high-danger tumor people, early tumor diagnosis and dynamic observation of curative effect prognosis.
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Description

technical field

[0001] The invention belongs to the field of medical examination and relates to serological detection technology, in particular to a quantitative detection technology of trace circulating DNA based on fluorescent dye. Background technique

[0002] Tumor is a disease that seriously endangers human health, and it has been the second leading cause of death in my country. Early diagnosis and treatment of tumors is the key to improving the cure rate. Recent studies have shown that during the growth of tumors, they can continuously release tumor cell DNA into the peripheral blood, so the circulating DNA content of patients can be higher than normal, and there are tumor-specific molecular genetic changes. Circulating DNA is cell-free DNA present in peripheral circulating serum / plasma. The level of circulating DNA can reflect the presence and severity of tumors, and is expected to become a new broad-spectrum tumor molecular marker.

[0003] The content of circulat...

Claims

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