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Typing detection kit for Candida albicans

A detection kit and technology for Candida, which is applied in the field of detection kits for the diagnosis of various common pathogenic Candida, can solve problems such as omissions and less subtypes, achieve good flexibility, facilitate production and operation, and shorten detection time Effect

Active Publication Date: 2014-08-27
上海凯普医学检验所有限公司 +2
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, PCR-based molecular biology techniques detect Candida only detect one or several of them, covering a small number of subtypes, which often easily leads to omissions in detection

Method used

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  • Typing detection kit for Candida albicans
  • Typing detection kit for Candida albicans
  • Typing detection kit for Candida albicans

Examples

Experimental program
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Embodiment Construction

[0073] The present invention will be described in detail below through examples.

[0074] In the examples, 20% EDAC solution, 0.1% SDS, 0.25% skim milk powder, 0.05% thimerosal, and 0.05% sodium azide all refer to the mass ratio, and 0.1% Tween 20 refers to the volume ratio.

[0075] Step 1 is used to detect the preparation of Candida gene chip

[0076] Step 1-1 Probe Design

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Abstract

The invention discloses a typing detection kit for Candida albicans. The typing detection kit comprises a gene chip and various primers. The gene chip is provided with 13 different types of nucleotide sequence probes for the Candida albicans, a DNA sequence marked with biotin points and a DNA sequence with a coding beta-globulin part, wherein the probes are in the sequences of SEQ ID Nos: 1-13 or sequences complementary with the SEQ ID Nos: 1-13, the DNA sequence with the biotin points is SEQ ID No. 14, and the DNA sequence with the coding beta-globulin part is SEQ ID No. 15. The various primers are in the sequences of SEQ ID Nos: 16-21. By means of the typing detection kit for the Candida albicans, a typing joint detection platform for detecting the Candida albicans is provided, synchronous joint detection can be achieved, the detection of specificity can be enhanced, the cost can be lowered, the detection time can be shortened, and the typing detection kit has great significance for carrying out clinic early diagnosis and population screening for the Candida albicans.

Description

technical field [0001] The invention relates to a kit, in particular to a detection kit for diagnosing various common pathogenic candida. Background technique [0002] Candida (Candida Albicans, Latin: Nostoc ） It is the most common opportunistic pathogen in fungi, also known as Candida. It often parasitizes on people's skin, oral cavity, vagina and intestinal mucosa, etc. When the body's immune function is low or the micro-ecological environment of the normal habitat is out of balance, it is easy to cause candidiasis. Candida can cause superficial skin and mucous membrane or systemic infection. Different parts of the infection can cause different diseases. In addition to skin candidiasis, there are also candidal stomatitis, vaginitis, cystitis, pyelonephritis, meningitis, Bacteremia and biliary tract infection. In recent years, due to the increasing number of immunocompromised populations (including malignant tumors, blood diseases, AIDS, diabetes, autoimmune diseases, s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6837C12Q1/686C12Q2565/501C12Q2545/101
Inventor 郭亮朱娟娟谢龙旭
Owner 上海凯普医学检验所有限公司
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