5 results about "Beta globulins" patented technology

The invention discloses an agricultural composition for preventing and treating powdery mildew of cucumbers, and belongs to the technical field of pesticides. The effective components of the agricultural composition provided by the invention consist of the physcion and the beta-lupin globulin polypeptide, and the mass ratio of the physcion to the beta-lupin globulin polypeptide is (4: 1)-(1: 8). The two components are compounded for use within the ratio range to achieve a synergistic effect.

The invention discloses a molecular simulation method for analyzing a milk powder dissolution mechanism, and belongs to the technical field of food science and molecular simulation. The method comprises the following steps: obtaining structures of lactose, alpha s1-casein, alpha s2-casein, beta-casein and beta-lactoglobulin, and carrying out pretreatment; the method comprises the following steps: predicting an active pocket through CB-Dock2, and carrying out molecular docking by utilizing AutoDock Vina to obtain a binding conformation; constructing a compound system according to a specific molar ratio, and performing energy minimization and 100ns molecular dynamics simulation by adopting GROMACS to generate a trajectory file; calculating combined free energy based on an MM-PBSA method; and finally, analyzing RMSD, RMSF, SASA and hydrogen bond parameters, and combining free energy to jointly reveal the dissolution mechanism. The method systematically and dynamically analyzes the interaction between the multi-component milk protein and the lactose by optimizing the set molar ratio of the protein to the lactose, overcomes the defects that a traditional method is not systematic and high in cost and existing simulation lacks a dynamic view angle, and provides a molecular theoretical basis for optimization of a milk powder processing technology.

The invention provides a signal peptide combination for improving antibody expression quantity, a recombinant expression vector, a recombinant cell and application, and belongs to the technical field of bioengineering. According to the invention, three signal peptides SP-A, SP-B and SP-C with good expression are combined pairwise and fused with an Inflixi or human immune globulin antibody in Chinese hamster ovary cells for expression. Compared with the original carrier signal peptide, the combined signal peptide has the advantages that the antibody expression quantity is obviously improved, and the problem of low antibody expression quantity is solved. The antibody produced by the method is high in yield and convenient for industrial application.

This invention relates to the field of medical information processing technology, and more particularly to a method, system, and platform for predicting gamma globulin-unresponsive Kawasaki disease. Clinical data from affected children is collected, preprocessed, and feature-selected to obtain eight key features. An ensemble learning algorithm is used to construct and validate a prediction model. During prediction, the eight features are input into the model, and the output risk probability is used. The results are then interpreted using SHAP (Shape Algorithm for Predicting Risk) to generate a visual representation. The system is deployed as a prediction platform via a web framework. This invention is based on multi-center data, exhibits strong generalization ability and good interpretability, and is easy to apply rapidly in clinical practice.

The invention discloses a construction method and application of a membrane-bound IL-21 K562 cell strain, a human IL-21 mature sequence is fused with an IgG4 hinge region, a human immune globulin gamma-4 chain CH2 / CH3 region and a CD4 transmembrane structural domain through genetic engineering to construct an mbIL-21 fusion gene, and the mbIL-21 fusion gene is cloned to a PT-mnud carrier by using BamH I / Xho I double enzyme cutting sites to obtain a recombinant plasmid PCDH-mbIL-21. HEK 293T cells are transfected through a lentivirus packaging system, high-titer lentivirus particles are prepared, and the cell strain stably expressing mbIL-21 is obtained through puromycin screening after K562 cells are infected. The mbIL-21 K562 cell strain constructed by the invention has the characteristics of long acting, targeting and stable expression, and provides a powerful tool for basic research and clinical transformation of immunotherapy.