57 results about "Beta globulins" patented technology

The present invention provides methods for the quantification of human, human chimeric, humanized antibodies or fragments thereof without the use of target specific molecules. More specifically, the present invention relates to a quantitative assay comprising the step of blocking the non-specific binding sites of a capture agent with a blocking buffer comprising a non-human mammalian globulin such as bovine gamma globulin (BGG).

The invention discloses a cell cryopreservation medium for dendritic cells. An effective amount of cryoprotective agents, cell stabilizers and anti-setting agents are added into RPMI-1640 culture medium and uniformly mixed to form the cell cryopreservation medium for the dendritic cells. The cryoprotective agent is dimethyl sulfoxide, and the cell stabilizer is human gamma globulin; the anti-setting agent is poly-L-lysine, and the molecular weight range of the poly-L-lysine is 30000 to 70000; and the volume percentage concentration of the dimethyl sulfoxide is 1 to 3 percent. When used for cryopreserving DCs, the cell cryopreservation medium disclosed by the invention can ensure the cryopreservation revival rate and immunocompetence of the DCs, and the cryopreservation revival rate and the immunocompetence are not significantly decreased within one year of cryopreservation; the human gamma globulin as the cell stabilizer can maintain the immunocompetence of the DCs; the poly-L-lysine as the anti-settling agent can prevent the human gamma globulin from settling to the bottom of the cell cryopreservation medium in the process of storage, and moreover, the poly-L-lysine has part of the effect of the cryoprotective agent, and can also help to maintain the immunocompetence of the DCs.