22 results about "Beta globulins" patented technology

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The invention discloses an agricultural composition for preventing and treating powdery mildew of cucumbers, and belongs to the technical field of pesticides. The effective components of the agricultural composition provided by the invention consist of the physcion and the beta-lupin globulin polypeptide, and the mass ratio of the physcion to the beta-lupin globulin polypeptide is (4: 1)-(1: 8). The two components are compounded for use within the ratio range to achieve a synergistic effect.

Antigen-binding molecules engineered to replace the light chain constant domain (CL) and heavy chain constant domain 1 (CH1) with HLA class I histocompatibility antigen alpha chain-E alpha-3 (HLA-E) / beta2 microglobulin (B2M) or intercellular adhesion molecule 1 domain 1 (ICAM-1 D1) / ICAM-1 D1 dimerization domain are described.

The invention discloses a preparation method of beta-lactoglobulin fibers, which is characterized by comprising the following steps: S1, placing a 10 mg / mL beta-LG solution in a room temperature environment, continuously stirring for 2 hours by adopting a magnetic stirrer, then storing at 4 DEG C, completing an overnight hydration process, and performing centrifugal operation to obtain a product A; s2, preparing a product A solution with a certain concentration, and adding the product A solution into a plasma reaction kettle; and S3, setting the input voltage U of the plasma reaction kettle, maintaining the current within 1 + / -0.002 A, and controlling the reaction temperature T and the treatment time t to obtain the finished product P-beta-LGF. The method can effectively improve the fibrosis degree of the milk protein fiber during preparation, and the prepared beta-lactoglobulin fiber has better structural characteristics and functional characteristics.

The invention provides an anti-human papilloma virus (HPV) contraception device and a production process thereof. The contraception device is composed of a latex material body, lubricating liquid and an aluminum foil package, the lubricating liquid contains anhydridized bovine beta-lactoglobulin and beta-glucan, viruses can be adsorbed, micro-ecological balance can be maintained, and the anti-HPV function is achieved. The production process comprises the working procedures of raw material preparation, forming, inspection, filling, sterilization and packaging. A special device is adopted for packaging and comprises a feeding assembly, a material pushing and empty bag preventing structure, a smoothing unit and a heat sealing component, the contraception device can be prevented from deviating or inclining, and the packaging consistency and the sealing quality are improved. The device has contraception and virus protection functions, and the production process is beneficial for improving the qualified rate of finished products and the production efficiency.

The present disclosure relates generally to methods and compositions for producing a heterologous globulin of interest in a recombinant filamentous fungal cell. Thus, certain embodiments relate to compositions and methods for the production of globulins, recombinant filamentous fungal strains comprising a globulin productivity enhancing phenotype, polynucleotides (e.g., expression constructs) encoding one or more globulins, industrial scale fermentation, globulin recovery processes, and the like.

The application discloses a beta-lactoglobulin mutant, a preparation method and application, relates to the technical field of genetic engineering, and the amino acid sequence of the beta-lactoglobulin mutant is shown as SEQ ID NO. 1; the preparation method is as follows: designing a forward primer, a reverse primer, a forward mutant primer and a reverse mutant primer; using a wild-type beta-Lg plasmid as a template, a mutant DNA segment is obtained by using overlap extension PCR technology; after the mutant DNA segment is connected with an empty vector, the obtained recombinant plasmid is transformed into an E. coli competent cell, cultured and identified; the recombinant plasmid is transformed into the E. coli competent cell, cultured and lysed; the cell supernatant is collected, purified, and the beta-lactoglobulin mutant is obtained; the application improves the binding property of the beta-lactoglobulin with specific IgE antibodies, enhances the immunological activity of the beta-lactoglobulin allergen, and enhances the sensitivity and specificity of the allergen specific IgE antibody detection result.

The invention discloses a molecular simulation method for analyzing a milk powder dissolution mechanism, and belongs to the technical field of food science and molecular simulation. The method comprises the following steps: obtaining structures of lactose, alpha s1-casein, alpha s2-casein, beta-casein and beta-lactoglobulin, and carrying out pretreatment; the method comprises the following steps: predicting an active pocket through CB-Dock2, and carrying out molecular docking by utilizing AutoDock Vina to obtain a binding conformation; constructing a compound system according to a specific molar ratio, and performing energy minimization and 100ns molecular dynamics simulation by adopting GROMACS to generate a trajectory file; calculating combined free energy based on an MM-PBSA method; and finally, analyzing RMSD, RMSF, SASA and hydrogen bond parameters, and combining free energy to jointly reveal the dissolution mechanism. The method systematically and dynamically analyzes the interaction between the multi-component milk protein and the lactose by optimizing the set molar ratio of the protein to the lactose, overcomes the defects that a traditional method is not systematic and high in cost and existing simulation lacks a dynamic view angle, and provides a molecular theoretical basis for optimization of a milk powder processing technology.

The invention provides a signal peptide combination for improving antibody expression quantity, a recombinant expression vector, a recombinant cell and application, and belongs to the technical field of bioengineering. According to the invention, three signal peptides SP-A, SP-B and SP-C with good expression are combined pairwise and fused with an Inflixi or human immune globulin antibody in Chinese hamster ovary cells for expression. Compared with the original carrier signal peptide, the combined signal peptide has the advantages that the antibody expression quantity is obviously improved, and the problem of low antibody expression quantity is solved. The antibody produced by the method is high in yield and convenient for industrial application.

The invention discloses an anti-HPV contraceptive product and a preparation process, and belongs to the technical field of contraceptive products. The latex base material is formed by vulcanizing and cross-linking natural latex and an epoxy chloropropane cross-linking agent; the composite lubricating liquid contains anhydridized bovine beta-lactoglobulin and beta-glucan, the anhydridized bovine beta-lactoglobulin and the beta-glucan form a synergistic anti-HPV system, and the combination of HPV virus L1 protein and a host cell surface receptor can be specifically blocked. The preparation process comprises the steps of raw material preparation, latex base material forming and vulcanization, demolding, lubricating liquid preparation, filling and sterilization. The product disclosed by the invention not only has a reliable physical contraception function, but also can efficiently and specifically inhibit HPV virus infection through the synergistic effect of specific active ingredients, and provides dual protection for a user.

The present disclosure provides a method of determining one or more parameters of an injection of a formulation in a human subject, the method comprising: administering to an animal subject via subcutaneous injection from a high volume autoinjector about 3 mL to about 50 mL of a formulation comprising a therapeutically effective amount of a hyaluronidase enzyme; measuring one or more parameters of the subcutaneous injection in the animal subject; and determining the same one or more parameters of the subcutaneous injection in the human subject from the one or more measured parameters from the animal subject; wherein the one or more parameters are selected from injection time, injection force, and injection site reaction data.

This invention relates to the field of medical information processing technology, and more particularly to a method, system, and platform for predicting gamma globulin-unresponsive Kawasaki disease. Clinical data from affected children is collected, preprocessed, and feature-selected to obtain eight key features. An ensemble learning algorithm is used to construct and validate a prediction model. During prediction, the eight features are input into the model, and the output risk probability is used. The results are then interpreted using SHAP (Shape Algorithm for Predicting Risk) to generate a visual representation. The system is deployed as a prediction platform via a web framework. This invention is based on multi-center data, exhibits strong generalization ability and good interpretability, and is easy to apply rapidly in clinical practice.

The invention discloses related application of beta2 microglobulin in fat cells as a target spot for treating obesity, and relates to the field of biological medicine. The beta2 microglobulin in the adipocyte can be used as a target for treating obesity, the effect of effectively preventing, treating or improving obesity and / or complications thereof can be achieved by reducing expression of the beta2 microglobulin in the adipocyte, and the beta2 microglobulin has the advantages of high safety, good treatment effect and the like.

The invention discloses a medicine formula for treating chronic obstructive pulmonary disease, and relates to the technical field of traditional Chinese medicine formulas. Gamma globulin and interferon in the human placenta can prevent and control various infections and regulate human immunity, and the radix asteris decoction has the effect of eliminating phlegm and also has a certain effect of relieving cough; alpha-linolenic acid of the perilla seed oil can reduce anaphylactic reaction; the medicines are compatible to achieve the effects of relieving asthma and cough and tonifying lung and kidney; the traditional Chinese medicine composition is wide in indication and suitable for the COPD lung and kidney deficiency type, the type is the main symptom type in the COPD remission stage, yang deficiency, blood stasis and turbid phlegm are taken into consideration, compared with ginseng gecko powder which only tonifies qi and yin deficiency, the traditional Chinese medicine composition can be applied in the early stage after acute stage remission, and the traditional Chinese medicine composition has wider indication; the traditional Chinese medicine composition is mainly prepared from powder, is convenient to decoct and take, can be taken for a long time, is good in patient compliance, is refined in medicine, saves the treatment cost of patients and medical resources, and is high in cost performance.

A diagnostic immunoassay reagent composition comprising nanoparticles formed from polymeric gamma globulin is disclosed. The nanoparticle has at least one functional group on its surface, and at least one analyte-specific binding partner is attached to the nanoparticle through the at least one functional group. Also disclosed are kits, devices, and systems comprising the diagnostic immunoassay reagent compositions, as well as methods of producing and using the diagnostic immunoassay reagent compositions.

In variants, the method of generating a hypoimmunogenic cell can include: integrating an insertion sequence into a cell genome (e.g., into a Beta-2-Microglobulin (B2M) gene) and performing a cell selection. The method can optionally include: integrating a selection sequence into the cell genome, removing a selectable marker, editing an additional gene, and / or any other suitable steps. The hypoimmunogenic cell can include a genetically engineered sequence including: a Beta-2-Microglobulin:human leukocyte antigen (B2M:HLA) fusion gene, a multicistronic element, and a kill switch gene.

The invention belongs to the technical field of biological peptide evaluation, and discloses a machine learning-based beta-lactoglobulin ACE inhibitory peptide multi-attribute evaluation method. The method comprises the following steps: 1, collecting related data of the beta-lactoglobulin ACE inhibitory peptide, and constructing a multi-attribute evaluation model; step 2, acquiring related data of the target beta-lactoglobulin ACE inhibitory peptide, and outputting an evaluation value of each attribute; 3, obtaining the contribution degree of each attribute according to the demand scene, and correcting the evaluation value of each attribute based on the contribution degree; 4, obtaining a comprehensive evaluation value according to the influence coefficient; 5, performing grading evaluation based on the comprehensive evaluation value; step 6, adjusting and optimizing a grading evaluation result; according to the method, the influence coefficients of different attributes can be dynamically generated according to the mutual influence condition of different attributes in different scenes, so that the evaluation value is corrected, and the evaluation of the beta-lactoglobulin ACE inhibitory peptide is more accurate.

The application discloses a beta-lactoglobulin fluorescent probe, a preparation method and application thereof, and the structural formula of the probe is (C 30 H 25 N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, triggers significant enhancement of a fluorescence signal, and realizes low-background fluorescence detection of the beta-lactoglobulin. The probe has low cytotoxicity, has excellent mitochondrion targeting capability, and provides a powerful tool for visualization of the beta-lactoglobulin in living cells. After being combined with the beta-lactoglobulin, the sample produces obvious color change under ultraviolet light irradiation, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, and further realizing rapid detection of milk protein level in beverages. The beta-lactoglobulin fluorescent probe has wide application prospects in the fields of biological imaging, milk protein detection and beverage quality control.

The present disclosure relates to cell-type restricted or -specific inhibition of transforming growth factor-beta receptor 2-mediated responses. In particular there is provided for a conjugate, for example a fusion protein, comprising a first moiety, which is an immunoglobulin chain variable domain which is capable of specifically binding transforming growth factor-beta receptor 2, and at least a second moiety capable of binding a target, for example, expressed on the cell surface of a target cell that expresses transforming growth factor-beta receptor 2, and wherein the first moiety on its own, i.e. in the absence of the second moiety, is unable to inhibit TGFB signaling in such cell. Also provided is for the treatment of a disease or condition with the conjugate.

The invention discloses a construction method and application of a membrane-bound IL-21 K562 cell strain, a human IL-21 mature sequence is fused with an IgG4 hinge region, a human immune globulin gamma-4 chain CH2 / CH3 region and a CD4 transmembrane structural domain through genetic engineering to construct an mbIL-21 fusion gene, and the mbIL-21 fusion gene is cloned to a PT-mnud carrier by using BamH I / Xho I double enzyme cutting sites to obtain a recombinant plasmid PCDH-mbIL-21. HEK 293T cells are transfected through a lentivirus packaging system, high-titer lentivirus particles are prepared, and the cell strain stably expressing mbIL-21 is obtained through puromycin screening after K562 cells are infected. The mbIL-21 K562 cell strain constructed by the invention has the characteristics of long acting, targeting and stable expression, and provides a powerful tool for basic research and clinical transformation of immunotherapy.

The invention discloses an electrochemical immunosensor for detecting bovine beta-lactoglobulin and a preparation method of the electrochemical immunosensor. The sensor comprises an electrode, a composite material is deposited on the surface of the electrode, an anti-bovine beta-lactoglobulin antibody is coupled to the composite material, and the composite material comprises reduced graphene oxide modified by a Ni-containing metal organic framework material. The preparation method of the sensor comprises the following steps: dissolving nickel salt and naphthalene dicarboxylic acid in an organic solvent to obtain a first solution; dispersing the amination modified reduced graphene oxide in a first solution, and carrying out a heating reaction to obtain an rGO-TEPA coated Ni-MOF composite material; the rGO-TEPA-coated Ni-MOF composite material is electrically deposited on the surface of the activated electrode, and a modified electrode is obtained; an anti-bovine beta-lactoglobulin antibody is coupled to the surface of the modified electrode to obtain the electrochemical immunosensor, and the electrochemical immunosensor is low in bovine beta-Lg detection limit and wide in linear range and has good selectivity, reproducibility, stability and anti-interference capacity.