43 results about "Polystyrene nanoparticles" patented technology

The invention provides a preparation method of a superhydrophobic coating. The preparation method comprises the steps of 1, mixing 50-80 parts of hydrophobic nanoparticle dispersing liquid with the mass percentage of 2-15%, 20-50 parts of polystyrene nanoparticle dispersing liquid with the mass percentage of 1-10% and 0-30 parts of solvent according to the volume part ratio, and ultrasonically dispersing the mixture to obtain a mixed solution; 2, forming the mixed solution on the surface of a substrate and in a microstructure in the substrate; 3, drying the substrate obtained in the step 2, then, raising the temperature to 160-230 DEG C, baking to melt polystyrene nanoparticles, and taking out for naturally airing. The invention also relates to a superhydrophobic paint and the superhydrophobic coating.

The invention relates to a preparation method of nanostructured array chip with surface plasma for resonance imaging. The preparation method is characterized by comprising the following steps: (1) selecting and cleaning a substrate; (2) using a vacuum evaporated coating to coat a first metal film on the substrate; (3) self-assembling a layer of polystyrene nanoparticles on the metal film; (4) coating a second metal film in a way of evaporation on the self-assembled layer so as to fill the gas between balls; (5) eliminating the nanoparticles with the Lift off technology; (6) using laser direct writing machine to manufacture a lattice mask plate of the level of micron; (7) adopting the photolithography to transmit the mask figure to the substrate; (8) acquiring the lattice figure through developing and stripping of photoresist; (9) using a chemical method to eliminate the excessive metal and photoresist; thus the metal lattice chip of the cyclical nanostructure can be formed. The method adopts the polystyrene nanoparticles for self-assembly so as to prepare the cyclical nanostructure, and thus is suitable for massive production of the nanostructure.

Size-controlled immobilization of metal nano-clusters onto particles or nanoparticles is achieved using a polyol process. Polyol processing makes it possible to use thiol groups as a chemical protocol to functionalize the surface of particles, such as silica and polystyrene nanoparticles. Metal nano-clusters, such as silver, gold, platinum and palladium, nucleate and grow on the surface of the particles. The metal nano-clusters may be synthesized in a one-pot process from metal salts, nitrates, nitrites, sulfates, sulfites and the like. Any source of metal ions compatible with the polyol suspension and selected particles may be used. The size of immobilized metal nano-clusters may be controlled by additions of a poly(vinylpyrrolidone) or other polymer capable of regulating the metal ion reduction and nucleation process and by controlling concentration of metal ions, the nucleation and / or growth temperatures, and processing time.

The invention discloses a magnetic Fe3O4@PS-TMT nanoparticle with an adsorption function and a preparation method thereof. The magnetic Fe3O4@PS-TMT nanoparticle with the adsorption function is a composite nanoparticle of ferroferric oxide nanoparticle aggregate surface-grafted styrene-mercapto-s-triazine resin; and the preparation method comprises the following steps: preparing a chloromethylatedmagnetic polystyrene nanoparticle by an emulsion polymerization method; and immobilizing a mercapto-s-triazine functional group on the surface of the magnetic polystyrene nanoparticle by a nucleophilic substitution reaction to prepare the magnetic Fe3O4@PS-TMT nanoparticle with the adsorption function. The magnetic Fe3O4@PS-TMT nanoparticle with the adsorption function provided by the invention has rich mercapto-s-triazine functional group on the surface, can rapidly adsorb a large number of heavy metal ions from an aqueous solution, and has relatively high saturated adsorption capacity for heavy metal ions such as mercury, lead, cadmium, chromium, manganese, nickel and copper; and the preparation method provided by the invention is simple, mild in condition, economical and environmentally friendly.

The invention relates to a preparation method for a protective coating. The preparation method comprises the following steps: preparing a mixed solution a containing 1 to 50 g / L of polyelectrolyte and 0.01 to 2 mol / L of strong electrolyte; allowing the mixed solution a to be formed on the surface of the matrix and microstructures in the matrix and drying the matrix; mixing 50 to 100 parts of polytetrafluoroethylene nanoparticle dispersion liquid with a mass fraction of 2 to 15% and 0 to 50 parts of polystyrene nanoparticle dispersion liquid with a mass fraction of 1 to 10% and carrying out stirring or ultrasonic dispersion so as to obtain a mixed solution b; allowing the mixed solution b to be formed on the surface of the matrix and the microstructures in the matrix and drying the matrix; and heating the matrix to 160 to 220 DEG C for baking so as to allow polystyrene particles to be fused and polytetrafluoroethylene particles to be cured, and then taking the matrix out for natural cooling. Moreover, the invention also relates to the protective coating, a filter material and the matrix.

The invention relates to a method for preparing a solar cell surface absorption enhancement layer, belonging to the technical field of solar cells. For the problem of low efficiency of a TiO2 light quantum, the invention provides the method for preparing the solar cell surface absorption enhancement layer. An opal structure film is prepared by configuring a polystyrene nanoparticle emulsion and allowing TiO2 to penetrate into the opal structure film with the film as a template, the opal structure film is removed through sintering, and a TiO2 film with an inverse opal structure is prepared, then silver nanoparticles are deposited in the gap of the TiO2 film with the inverse opal structure, the silver nanoparticles are used to inhibit an electron hole compound effect and improve a light absorption enhancement effect, finally the processing is carried out by using a titanium tetrachloride solution, a special net nano porous structure is formed at the surface of the film, the electron mobility is improved, an electron life is prolonged, and the solar cell surface absorption enhancement layer is prepared.

The invention relates to a method for determining ultramicro filter membrane pore size and pore size distribution. The method comprises the following steps: 1) selecting a primary standard substance; 2) scanning maximum absorption wavelengths of nanoparticles with various particle sizes in an ultraviolet-visible wavelength range, and accordingly drawing a standard curve under the maximum absorption wavelengths; 3) selecting polystyrene nanoparticles with single particle size and preparing into solution with mass concentration of C0, uniformly dispersing the nanoparticles with the particle size in water by adopting ultrasound, carrying out a filtration experiment on an ultramicro filter membrane by adopting a suspension liquid filtration method, selecting filtered solution, determining absorbance of the polystyrene nanoparticles with the particle size under the maximum absorption wavelength, calculating concentration Ct of the nanoparticles with the particle size in the filtered solution by adopting a standard curve, and further calculating retention rate R of the ultramicro filter membrane on the polystyrene nanoparticles on the polystyrene nanoparticles with the particle size; and 4) selecting the polystyrene nanoparticles with different particle sizes, repeating the step 3), retaining, and calculating membrane pore diameter of the ultramicro filter membrane according to the determined retention rate. The method provided by the invention is simple in principle and easy to operate and can determine the ultramicro filter membrane pore size and the pore size distribution more accurately.

Size-controlled immobilization of metal nano-clusters onto particles or nanoparticles is achieved using a polyol process. Polyol processing makes it possible to use thiol groups as a chemical protocol to functionalize the surface of particles, such as silica and polystyrene nanoparticles. Metal nano-clusters, such as silver, gold, platinum and palladium, nucleate and grow on the surface of the particles. The metal nano-clusters may be synthesized in a one-pot process from metal salts, nitrates, nitrites, sulfates, sulfites and the like. Any source of metal ions compatible with the polyol suspension and selected particles may be used. The size of immobilized metal nano-clusters may be controlled by additions of a poly(vinylpyrrolidone) or other polymer capable of regulating the metal ion reduction and nucleation process and by controlling concentration of metal ions, the nucleation and / or growth temperatures, and processing time.

The invention discloses a preparation method of a super-hydrophobic polyaniline / polystyrene composite micro-nano material. The preparation method comprises the following steps: dissolving and dispersing styrene in an aqueous surfactant system, and dropwise adding an initiator to prepare polystyrene nanoparticles with diameter smaller than 100 nanometers; and then adding the polystyrene nanoparticles into a surfactant in which aniline is dissolved and allowing reaction while stirring to obtain the super-hydrophobic polyaniline / polystyrene composite micro-nano material. The preparation method has simple operation, provides obtained polyaniline / polystyrene composite micro-nano tubes with electrical conductivity of polyaniline and toughness of polystyrene as a result of adding the surfactant. And length, width and diameter of the polyaniline / polystyrene composite micro-nano materials can be controlled by adjusting concentration of the surfactant, which provides strong support for fabrication of microelectronic devices.

The invention discloses a method for preparing a multi-stage hierarchical structure super-hydrophobic material, relates to a method for preparing a hierarchical structure super-hydrophobic material, and aims at solving the problems that the existing hierarchical structure super-hydrophobic material is just remained at the level of a micron-nanometer two-stage structure, and development of the characteristics of the super-hydrophobic performance caused by the multi-scale effect cannot be achieved. The method comprises the steps of: 1, preparing polystyrene nano particle sol solution; 2, preparing a; 3, preparing a sol emulsion of the precursor which is obtained in the step 2; 4, preparing sol crystal microspheres; 5, preparing a multi-stage hierarchical structure material; 6, performing the surface hydroxylation treatment of the multi-stage hierarchical structure material; 7, preparing the super-hydrophobic material. By adopting the preparation method, preparation of a micron-submicron-nanometer hierarchical structure material is achieved; the obtained material has excellent super-hydrophobic performance; the defect that the development of characteristics and functions of the multi-scale effect cannot be achieved is compensated; the method can be used for preparing the multi-stage hierarchical structure super-hydrophobic material.

The invention relates to a polystyrene nanoparticle preparation method which belongs to the nanometer materials preparation field. The existing polystyrene nanoparticle preparation method has the disadvantages of large dosage of surfactant, complex equipment, high reaction temperature and the like. The preparation method of the invention comprises the following steps: mixing the styrene, water, alkyl ammonium bromide cationic surfactant and cracked photoinitiator to emulsify, placing the mixture in ultraviolet radiation to cause polymerization, obtaining polystyrene nanoparticle by filtration or centrifugation; adding crosslinking agent in the polymerization reaction system to obtain polystyrene nanometer gel particle. In the invention water is used as a reaction medium, the polymerization can be performed at room temperature; and in the reaction system, the monomer concentration is high, the dosage of surfactant is less, the reaction rate is fast, the monomer conversion rate is high, and the equipment is simple and economic, thus being easy to realize large-scale industrialized production.

The invention belongs to the technical field of preparation of high polymer materials, particularly relates to a preparation method of water-soluble polystyrene nanoparticles. The preparation method comprises the following steps: (1) mixing styrene monomers and deionized water so as to obtain a mixed solution A; (2) sealing the mixed solution A in an irradiation vessel, feeding nitrogen, then carrying out irradiation crosslinking by using a cobalt source or an electron beam to obtain a product, and carrying out freeze-drying or vacuum drying on the product to obtain a final product. The water-soluble polystyrene nanoparticles are prepared by an irradiation one-step method, the synthesis method is quick, simple, low in cost and high in yield, and the synthesized polystyrene nanoparticles can be dissolved in water well.

The invention discloses a preparation method and application of a hollow composition template molecularly imprinted polymer micro-shell having a selective absorption effect on sulfanilamide and quinolone antibiotics. The preparation method of the micro-shell comprises the following steps: preparing polystyrene nano-particles, preparing a pre-assembly solution, preparing molecularly imprinted nanospheres, preparing a hollow polymer material, and removing template molecules. The hollow composition template molecularly imprinted polymer micro-shell prepared by the invention not only has the advantages of being good in molecular imprinted polymer selectivity, but also has the advantages of being large in absorption capacity, rapid in absorption rate, wide in application range and the like compared with other types of molecularly imprinted polymers. The preparation method of the hollow composition template molecularly imprinted material not only can solve the existing bottleneck problems ofthe molecularly imprinted material, but also can significantly broaden the selective absorption range of the hollow molecularly imprinted material, thereby widening the application range of the molecularly imprinted material.

The invention discloses a method for greatly improving chemiluminescence magnetic enzyme immunization sensitivity by depending on polystyrene nanoparticles. The method comprises the following steps of: covalently coupling one enzyme-labeled antibody of an antigen to be detected to a polystyrene latex nanoparticle; covalently coupling the other one enzyme-labeled antibody of the antigen to be detected to a magnetic particle; adding the two particles into a system comprising the antigen to be detected; forming a magnetic particle-antibody-antigen to be detected-enzyme-labeled antibody-polystyrene latex nanoparticle composite through the interaction of antibody-antigen-enzyme-labeled antibody; capturing the composite by a magnetic separation technique; and calculating the concentration of the antigen to be detected by detecting luminous intensity of a chemiluminescence substrate by using a chemiluminescence detector. The method has the advantages of high specificity, high sensitivity, high detection speed and the like.

The invention discloses a hollow carbon nanosphere material and a preparation method thereof, and belongs to the field of an inorganic material. According to the method, a controllable polystyrene nanoparticle is taken as a template, an organic polymer coating is coated on the surface of the polystyrene nanoparticle, and then the hollow carbon nanosphere material of which the carbon layer thickness is adjustable is obtained by adopting a high-temperature template removing method. The method is simple and feasible, economical and reasonable, and applicable to industrial production, and the sizes of carbon spheres and the thickness of the shell can be regulated and controlled.

The invention relates to the technical field of water-based fluorescent ink, and provides water-based fluorescent ink as well as a preparation method and application thereof in full-color printing and encryption anti-counterfeiting. The water-based fluorescent ink provided by the invention comprises the components: polystyrene nanoparticles, a surfactant, water, ethanol, glycerol and a UV-cured fluorescent dye, wherein the fluorescent dye is a red, green or blue fluorescent dye. The fluorescent ink with three colors of red, green and blue is prepared by adopting an instantaneous nano precipitation method, the high-viscosity fluorescent dye is dispersed and transferred from an oil phase to a water phase, and the obtained water-based fluorescent ink takes water as a main continuous phase, is good in safety and relatively low in viscosity, can be used for a high-flux civil ink-jet printer and can realize diversification and high efficiency of printed patterns. Patterns printed by the ink are completely invisible under natural light, different colors are displayed under ultraviolet light with different wavelengths, the ink can be used for full-color printing, the pattern quality is good, and the ink has wide application prospects in fluorescent color-changing encryption and anti-counterfeiting.

The invention relates to an antibody modified nanoparticle electrophoresis flowing type ELISA (enzyme linked immunosorbent assay) method and belongs to the field of biological materials. The method is characterized in that a first antibody attaches to the surface of a porous film, and sealant protein absorption is performed; antigen-first antibody reaction is performed; electrophoresis is used to drive the antibody to modified nanoparticles to perform second antibody-antigen reaction, and developing quantification is performed on an enzyme-labeled second antibody; the porous film is cleaned after the electrophoresis is used to drive the second antibody-antigen reaction, and the obtained porous film substrate is placed into the substrate solution of second antibody labeling enzyme for developing; antigen quantitative analysis is performed by detecting light absorbency. The method has the advantages that positive PDDA / PSS is used to modify a cellulose acetate membrane PEMs-CA, and carboxyl polystyrene nanoparticles are used as the carriers modified by the enzyme-labelled second antibody; PEMs modification lowers nonspecific absorption, antibody modified nanoparticles can amplify signals, electrophoresis drive force allows the second antibody to locally concentrates to the periphery of the antigen in a short time, and fast and sensitive detection is achieved.

The invention relates to gold-coated polystyrene nanoparticles and preparation method thereof. The Gold-coated polystyrene nanoparticles contain styrene monomer (purified), a camellia oil emulsifier, a gold chloride crosslinking agent, polyethylene glycol 6000, polyether triol, sodium periodate, sodium hydroxide, white phosphorus-ether saturated solution, and distilled water. The preparation method of the gold-coated polystyrene nanoparticles comprises the following steps: styrene monomer purification, camellia oil emulsifier preparation, gold chloride crosslinking agent preparation, styrene monomer polymerization, crosslinking of polystyrene particles and gold chloride crosslinking agent, and size screening of gold-coated nanoparticles. The gold-coated polystyrene nanoparticles prepared by the invention can participate in immunoturbidimetry as antigen or antibody carrier to amplify the sensitivity for determining a target antigen or antibody by geometric progression so as to achievedirect determination of microgram-scale or nanoscale target antigens or antibodies which can be determined only by complex chemiluminescence and enzyme-linked immunoassay method through a biochemical analyzer, thereby ensuring good actual use effect.

The invention discloses a preparation method of an asymmetric hollow porous composite material, which comprises the following steps: taking polystyrene spheres as a substrate, adding an organic silicon precursor, a stabilizer, an initiator and a catalyst, and reacting to obtain organic silicon-polystyrene nanoparticles with asymmetric structures; coating the organic silicon-polystyrene nanoparticles in a tris (hydroxymethyl) aminomethane hydrochloride solution by taking dopamine hydrochloride as a precursor to obtain an asymmetric composite material, and further performing high-temperature calcination in an inert gas environment to obtain the asymmetric hollow porous composite material. The method has simple steps and can realize large-scale production. The invention provides a novel idea for designing and preparing the symmetrical hollow porous composite material.

The invention relates to a preparation method of a polystyrene nanoparticle adsorbed on the mica sheet surface. The preparation method of the polystyrene nanoparticle comprises the following steps: 1, dissolving polystyrene of different molecular weights in a solvent to form a polystyrene solution with the concentration of 0.0005-0.01% g / ml, and removing the mica sheet matrix surface; 2, preparing a polystyrene nanoparticle with the average diameter of 80-190nm and the average height of 7-18nm on the mica sheet surface through a spin coating process; and 3, putting the prepared sample in a washed and dried culture dish, carrying out seal drying for 24-48h, and transferring the sample to a vacuum drier to preserve it. The dimension distribution of the nanoparticle is uniform; and the preparation method has the advantages of simple operation technology, good controllability, low cost, no need of complex chemical treatments, and no need of expensive equipment.

The invention discloses a kit for detecting keratoprotein 19 fragment and carcino-embryonic antigen contents in a sample based on a luminous quantum dot nanometer microemulsion probe. The kit comprises an immunochromatography detection test strip and the functional quantum dot nanometer microemulsion probe. The preparation method of the functional quantum dot nanometer microemulsion probe comprises the following steps of embedding or wrapping oil soluble quantum dots with emission wavelength between 520-620nm into modified polystyrene nanoparticles to acquire quantum dot nanometer microemulsion, covalently linking the quantum dot nanometer microemulsion with a detection antibody, and performing microemulsion treatment on the surface of the microemulsion to acquire the functional quantum dot nanometer microemulsion probe. The kit provided by the invention is based on novel fluorescence immunochromatography, avoids errors caused by identification by naked eyes, and can rapidly, synchronously, quantitatively and accurately detect a plurality of biological targets.

The invention discloses a magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as a preparation method and application of the magnetic photonic crystal microsphere. The microsphere is a core-shell type surface molecular imprinting magnetic inverse opal photonic crystal microsphere, wherein the core is a photonic crystal microsphere which is of a magnetic inverse opal structure and is formed by silicon dioxide nanoparticles, polystyrene nanoparticles and ferroferric oxide magnetic nanoparticles through self-assembly and high-temperature firing, and the shell is a molecular imprinting layer which is modified through a molecular imprinting technology and can specifically recognize aflatoxin B1. The prepared core-shell type surface molecular imprinting magnetic inverse opal photonic crystal microsphere can selectively extract aflatoxin B1 in a sample, and compared with a biological antibody modified material, the stability of the material can be greatly improved, and the preparation cost of the material is reduced.

The invention discloses a wood fiber capable of radiating far-infrared rays, which is characterized by comprising the following raw materials in parts by weight: 40-44 parts of wood fiber, 7-9 parts of fine wool cotton fiber, 5-8 parts of viscose fiber, 6-10 parts of Arenga engleri Beccari fiber, 20-25 parts of sugarcane peel, 17-19 parts of tea leftovers, 1-3 parts of infrared ceramic powder, 1.2-2.5 parts of polystyrene nanoparticle, 4-7 parts of carnauba wax, 10-13 parts of ethanol, 4-8 parts of polyvinylacetal, 0.3-0.7 part of dioctyltin dilaurate, 3-4 parts of markka, 2-4 parts of corydalis tuber, 1-2 parts of radix salviae miltiorrhizae, 3-6 parts of assistant and a right amount of water. By optimizing the production technique and adding the infrared ceramic powder, the wood fiber can radiate far-infrared rays, and has the advantages of diminishing inflammation, promoting the subsidence of swelling, resisting bacteria, enhancing the cell regeneration capacity and immunological cell functions and the like when acting on the human body. Besides, the multiple animal and plant fibers are added and mutually compounded, thereby saving the cost and enhancing the characteristics of the wood fiber. The prepared wood fiber is widely used in the fields of medical treatment and recovery.

The invention discloses a magnetically controlled surface-variable film comprising an electromagnet control layer, a glass supporting substrate layer, a variable microcolumn array, a polystyrene nanoparticle coating and a lubricating oil coating. The variable microcolumn array is located on the glass supporting substrate layer, and comprises two-dimensionally orderly and uniformly distributed andequidistantly arranged triangular pyramid-shaped microcolumns which are controlled by an electromagnet to stand or lie down, thereby achieving a function of controlling surface characteristics. Stepsfor preparing the surface-variable film include preparation of the variable microcolumn array, preparation of the polystyrene nanoparticle coating, and preparation of the lubricating oil coating.

The invention relates to a polystyrene nanoparticle preparation method which belongs to the nanometer materials preparation field. The existing polystyrene nanoparticle preparation method has the disadvantages of large dosage of surfactant, complex equipment, high reaction temperature and the like. The preparation method of the invention comprises the following steps: mixing the styrene, water, alkyl ammonium bromide cationic surfactant and cracked photoinitiator to emulsify, placing the mixture in ultraviolet radiation to cause polymerization, obtaining polystyrene nanoparticle by filtrationor centrifugation; adding crosslinking agent in the polymerization reaction system to obtain polystyrene nanometer gel particle. In the invention water is used as a reaction medium, the polymerization can be performed at room temperature; and in the reaction system, the monomer concentration is high, the dosage of surfactant is less, the reaction rate is fast, the monomer conversion rate is high,and the equipment is simple and economic, thus being easy to realize large-scale industrialized production.

The invention provides a preparation method of a superhydrophobic coating. The preparation method comprises the steps of 1, mixing 50-80 parts of hydrophobic nanoparticle dispersing liquid with the mass percentage of 2-15%, 20-50 parts of polystyrene nanoparticle dispersing liquid with the mass percentage of 1-10% and 0-30 parts of solvent according to the volume part ratio, and ultrasonically dispersing the mixture to obtain a mixed solution; 2, forming the mixed solution on the surface of a substrate and in a microstructure in the substrate; 3, drying the substrate obtained in the step 2, then, raising the temperature to 160-230 DEG C, baking to melt polystyrene nanoparticles, and taking out for naturally airing. The invention also relates to a superhydrophobic paint and the superhydrophobic coating.

The invention relates to an antibody modified nanoparticle electrophoresis flowing type ELISA (enzyme linked immunosorbent assay) method and belongs to the field of biological materials. The method is characterized in that a first antibody attaches to the surface of a porous film, and sealant protein absorption is performed; antigen-first antibody reaction is performed; electrophoresis is used to drive the antibody to modified nanoparticles to perform second antibody-antigen reaction, and developing quantification is performed on an enzyme-labeled second antibody; the porous film is cleaned after the electrophoresis is used to drive the second antibody-antigen reaction, and the obtained porous film substrate is placed into the substrate solution of second antibody labeling enzyme for developing; antigen quantitative analysis is performed by detecting light absorbency. The method has the advantages that positive PDDA / PSS is used to modify a cellulose acetate membrane PEMs-CA, and carboxyl polystyrene nanoparticles are used as the carriers modified by the enzyme-labelled second antibody; PEMs modification lowers nonspecific absorption, antibody modified nanoparticles can amplify signals, electrophoresis drive force allows the second antibody to locally concentrates to the periphery of the antigen in a short time, and fast and sensitive detection is achieved.

The invention provides a buffer layer of a functional shoe sole. The buffer layer is prepared from, by weight, 85-105 parts of polyisoprene, 9-15 parts of cyclohexyl thiomaleimide, 6-11 parts of thiuram disulfide, 8-17 parts of 1,4-diamino-butane, 16-25 parts of polyacrylamide, 23-29 parts of polystyrene nanoparticles, 31-45 parts of polylactic acid fiber, 8-15 parts of silicate acetate and 11-23parts of kaoline. Due to synergic cooperation of the components, the damping performance and wearing comfort of the shoe sole are greatly improved, and the shoe body is easier and attractive in wholeshape and capable of meeting the requirement of the public easily.

The invention discloses an alpha1 microglobulin trace detection method, and relates to the technical field of biological detection methods. The method comprises the following steps: mixing a reagent a with a sample, adding a reagent b, and carrying out mixed incubation; adding a reagent c for mixing, and reading an absorbance value; after timing reaction, reading the absorbance value again; calculating the difference value of the two absorbance values, making a regression equation function curve of the difference value to the concentration of the alpha1 microglobulin, and substituting the difference value of the sample to be detected into the function curve to calculate the concentration of the alpha1 microglobulin; the reagent a comprising polyethylene glycol and sodium zinc ethylene diamine tetraacetate; the reagent b comprising polyvinylpyrrolidone and sodium chloride; the reagent c comprising polystyrene nanoparticles only marked with an alpha1 microglobulin monoclonal antibody and polystyrene nanoparticles only marked with an alpha1 microglobulin polyclonal antibody. According to the method, loss of alpha1 microglobulin in the sample in the detection process is effectively reduced, detection errors are reduced through specific reagent use and proportion adjustment, and sensitivity is improved.