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Magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as preparation method and application of magnetic photonic crystal microsphere

A technology of magnetic photonic crystals and photonic crystal microspheres, which is applied in the field of separation science, can solve the problems of one-time use, extensive promotion and use restrictions, large consumption of organic reagents, cumbersome operation process, etc., to achieve selective enrichment and separation, overcome Reduced adsorption efficiency, simple and fast operation

Pending Publication Date: 2021-11-19
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the liquid-liquid extraction method has the disadvantages of large consumption of organic reagents, cumbersome and time-consuming operation, and poor specificity, while the solid-phase extraction method retains the target substance in the liquid sample under the action of the adsorbent, and can extract the target substance. The enrichment of substances is beneficial to improve the detection sensitivity, and the operation is simple, saving time and effort
AFB 1 The most commonly used extraction column for solid phase extraction is the immunoaffinity column, but its properties are unstable, relatively expensive, and it is limited in its widespread use due to one-time use.

Method used

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  • Magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as preparation method and application of magnetic photonic crystal microsphere
  • Magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as preparation method and application of magnetic photonic crystal microsphere
  • Magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as preparation method and application of magnetic photonic crystal microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of magnetic inverse opal photonic crystal microspheres:

[0044] Take 20% SiO 2 solution, 10% PS solution and 2.5% Fe 3 o 4 The solution was placed in a centrifuge tube, ultrasonically mixed into a homogeneous emulsion, transferred to a 5mL syringe, and another large 50mL syringe was filled with methicone. The two syringes were respectively fixed on the constant flow micro-flow pump, the microfluidic flow rate of the oil phase was 10mL / h, and the flow rate of the emulsion phase was 5mL / h. Using the principle of water-in-oil, methyl silicone oil cuts off the emulsion into micron-sized droplets and collects them in a plastic petri dish filled with methyl silicone oil (according to the literature: Journal of Chromatography A, 2020, 1626, 461379) . Afterwards, put the petri dish into a 60°C blast drying oven to dry at a constant temperature. After drying the water in the droplets, wash them with n-hexane and absolute ethanol for 3 to 4 times, and transfer...

Embodiment 2

[0049] The preparation method of Example 2 is the same as that of Example 1, except that acetic acid is used to adjust the pH to 6 when modifying the surface of the microspheres.

Embodiment 3

[0051] Using the surface-modified magnetic inverse opal photonic crystal microspheres prepared in Example 1 as the substrate, the molecularly imprinted magnetic microspheres were synthesized. The specific steps are as follows:

[0052] 1. Molecularly imprinted polymerization:

[0053] Using surface molecular imprinting technology, 10 mg of surface-modified microspheres were placed in a centrifuge tube, and template molecules, functional monomers, and cross-linking agents were added, and the volume was adjusted to 2 mL with a porogen (ie, the reaction solvent acetonitrile), and shaken at room temperature. The bed reacted for 15 minutes, and the rotation speed was 180 rpm. Then fill with nitrogen for 3 minutes, then add the initiator, seal the centrifuge tube with a parafilm, and finally put it into a shaker at 60° C. and 180 rpm for 24 hours. After the reaction is over, the reaction liquid is discarded under the action of a magnet to obtain microspheres coated with an organic ...

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Abstract

The invention discloses a magnetic photonic crystal microsphere for enriching and separating aflatoxin B1 as well as a preparation method and application of the magnetic photonic crystal microsphere. The microsphere is a core-shell type surface molecular imprinting magnetic inverse opal photonic crystal microsphere, wherein the core is a photonic crystal microsphere which is of a magnetic inverse opal structure and is formed by silicon dioxide nanoparticles, polystyrene nanoparticles and ferroferric oxide magnetic nanoparticles through self-assembly and high-temperature firing, and the shell is a molecular imprinting layer which is modified through a molecular imprinting technology and can specifically recognize aflatoxin B1. The prepared core-shell type surface molecular imprinting magnetic inverse opal photonic crystal microsphere can selectively extract aflatoxin B1 in a sample, and compared with a biological antibody modified material, the stability of the material can be greatly improved, and the preparation cost of the material is reduced.

Description

technical field [0001] The invention belongs to the field of separation science, in particular to a method for enriching and separating aflatoxin B 1 Magnetic photonic crystal microspheres and their preparation methods and applications. Background technique [0002] Aflatoxins (AFs) are a class of strong carcinogenic mycotoxins with serious hazards and stable physical and chemical properties, which have attracted much attention at home and abroad. It mainly enters human and animal bodies through dietary channels, and excessive intake can lead to liver cancer. In addition, aflatoxins can also cause growth disorders (such as stunted growth, emaciation, etc.). The International Agency for Research on Cancer (IARC) has classified aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ) and aflatoxin (AFG 2 ) is classified as a Class I carcinogen. Since the content of aflatoxin in the sample is usually very low, and the sample matrix is ​​complex, choosing...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N1/38G01N1/40G01N30/02G01N30/06G01N30/14G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/482G01N2030/484G01N1/34G01N1/405G01N1/4055G01N1/4077G01N1/38G01N1/40G01N30/02G01N30/06G01N30/14G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8679G01N2030/3007G01N2030/067G01N2030/324
Inventor 李建林王思伟李前进窦梦华邵瑞焦赛赛代士杰
Owner NANJING NORMAL UNIVERSITY
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