Method for determining content of beta2-microglobulin

Abstract

The invention discloses a method for determining the content of beta2-microglobulin. The method comprises the following steps: 1, preparing a test solution; the method comprises the following steps: taking 1ml of a sample, putting the sample into a 100ml measuring flask, adding a 6mol / L urea solution to a scale, standing at room temperature for 1 hour, and filtering by using a 0.45 mu m filter membrane, thereby obtaining the target product. 2, preparing a reference substance solution; the method comprises the following steps: taking a proper amount of a beta 2-microglobulin reference substance, putting the reference substance into a 100ml measuring flask, adding a 6mol / L urea solution to a scale, and standing at room temperature for 1 hour, so that each 1ml of the prepared solution contains 10mu g of beta 2-microglobulin; step 3, determining according to a high performance liquid chromatography; according to the present invention, the high performance liquid chromatography is adopted to perform determination, such that the beta 2-microglobulin result in the urine and the peritoneal effusion of the patient can be rapidly and conveniently determined, the important significance is provided for the monitoring of the beta 2-microglobulin in the patient body, and the important significance is provided for the artificial kidney research and development.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for measuring the content of β2-microglobulin. Background technique [0002] β2-microglobulin (β2-MG) is the light chain of human leukocyte antigen (HLA) class I antigen, a low-molecular-weight serum globulin produced by lymphocytes, platelets, and polymorphonuclear leukocytes, and exists in mature red blood cells and Various cell surfaces outside the placental trophoblast cells. β2-microglobulin can easily pass through the glomerular filtration membrane, and almost all of it is reabsorbed and degraded by the cells of the proximal convoluted tubule, and no longer flows back into the blood. The synthesis rate of β2-microglobulin and the release amount of cell membrane in healthy people are very constant, so the β2-microglobulin in the body remains stable. [0003] Detecting the concentration of β2-MG in blood or urine provides early, reliable and clinical d...

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Problems solved by technology

[0004] At present, the detection methods of β2-microglobulin are all immunological methods, including radioimmunoassay, enzyme-linked immunosorbent assay, time-resolved fluorescent immunoassay, immunotransmission method, immunoscatter turbidimetry, latex immunoscatter turbidimetry , latex-enhanced immune turbidimetry, etc., the kit needs to use a complete self-service biochemical analyzer of the matching model, which has strong limitations, and there is an urgent need for a method that can be detected using a general-purpose instrument

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