36 results about "NS - Normal saline" patented technology

The invention relates to a method for preparing a trivalent inactivated vaccine for preventing mink hemorrhagic pneumonia. The method is characterized by comprising the following concrete steps: separating serum G pseudomonas aeruginosa SD302, serum C pseudomonas aeruginosa SD312 and serum I pseudomonas aeruginosa SD403 from an ill mink body; storing bacteria by adopting a freeze-drying mode; respectively putting the three serum pseudomonas aeruginosa into PYG culture medium containing 2% fetal calf serum to cultivate at 37 DEG C; then carrying out enlarge cultivation, and carrying out inactivated treatment on a bacterium solution; diluting the inactivated bacterium solution until the concentration is 8.0*10<9> CFU / mL by using normal saline according to the bacteria content; mixing the three diluted inactivated bacterium solutions with 40% aluminum glue solution according to the volume ratio of 1:1:1:1; adding thiomersalate of which the final concentration is 0.004%, and mixing to obtain the trivalent inactivated vaccine. The method is safe, multi-titer, good in immunogenicity, good in specificity and the like, and is moderate in price, and applicable to substratum or clinical prevention of the mink hemorrhagic pneumonia.

The invention relates to the technical field of medicines, in particular to a stem cell preparation and an application of the stem cell preparation in preparing a drug for treating chronic nephritis. The provided stem cell preparation is prepared through resuspension of umbilical cord mesenchymal stem cells by the aid of normal saline, the content of urine protein of a patient and the number of urine erythrocyte are reduced by inhibiting expression of T cell surface antigen CD8 and secretion of INF-gamma and up-regulating secretion of IL-4 of T lymphocyte of peripheral blood, so that the effect for treating chronic nephritis is realized, the effective rate of treatment can reach 88%, and the remission rate can reach 54%.

The invention provides a female-rat vaginal-smear sample collection device. The female-rat vaginal-smear sample collection device comprises a vagina built-in head and a normal saline pusher. The quantitative normal saline pusher is adopted, and the consistency of normal saline for sampling is guaranteed. In the vagina built-in head, a cervix end and an orificium vaginae end are fixed for guaranteeing the consistency of a sampling part; a soft material is used in the cervix end, and operational friction stimulation is reduced; meanwhile, the flow direction of the normal saline entering the vagina can be changed to be outwards, the cervix is protected, infection caused when the normal saline reversely flows is avoided, and infection caused when cells of the inner wall of the vagina are injured is avoided in the mode that once washing is carried out with saline. In the operation process, the normal saline can be kept in the aseptic condition, and the animal infection rate in the operation process is reduced; the vagina built-in head can be disinfected and washed to be reused.

The invention relates to a sampling device, in particular to a dermatological scurf sampling device. The invention provides a dermatological scurf sampling device capable of automatically scraping scurf, automatically dripping normal saline and automatically clamping a glass slide. The dermatological scurf sampling device comprises a handheld frame body; first support frames which are arranged at the top ends of the two first telescopic rods; a scraper which is rotationally connected between the two first support frames; and two first telescopic rods which are both wound with the first springs, wherein first telescopic rods are arranged on the two sides of the handheld frame body, and the two ends of the first springs are connected with the first support frame and the handheld frame body correspondingly. A flow guide pipe channel on the front side is opened through an electromagnetic valve, normal saline drips on the scurf through the flow guide pipe channel on the front side, so that the scurf is kept active through the normal saline, and the effect that the scurf activity is kept through the normal saline is achieved.

The invention provides a detection system for intestinal feces. The detection method of the system includes: step 1, adding physiological saline and feces into the first centrifugal chamber; step 2, the stirring device of the first centrifugal chamber enters the stirring mode; step 3. The second liquid level sensor group starts to detect the liquid level height in the second centrifugal chamber; Step 4, the filtrate enters the second centrifugal chamber for centrifugation, and the primary centrifugal sediment after removing the supernatant enters the transfer chamber; Step 5, the second centrifugal chamber enters the emptying operation; Step 6, the secondary centrifugal sediment in the transfer chamber is returned to the first centrifugal chamber for centrifugation; Step 7, the transfer chamber enters the emptying operation; Step 8, the first control valve is opened again , the three centrifugal sediments enter the second centrifugal chamber; the physiological saline in the output storage bin flows into the second centrifugal chamber, and the second centrifugal chamber performs centrifugal work; Step 9, the fecal bacteria suspension that meets the requirements in the second centrifugal chamber enters the second centrifugal chamber Transit chamber storage.

The invention relates to a biological immune preparation for strengthening a goatpox vaccine antibody and a manufacturing method of the biological immune preparation. The steps adopted in the method include that the fresh spleen of a healthy yak is selected, after fat, the envelope and the internal connective tissue are removed, the spleen is subjected to freezing preservation, the yak spleen obtained after freezing preservation is taken out and melted and is minced through a tissue stamp mill, after sterile tri-distilled water with the same weight is added, the high-speed tissue stamp mill is used for conducting homogenizing at a high speed for two times, and homogenizing is conducted 10 min each time; and prepared homogenate is frozen at the temperature of minus 20 DEG C, freezing and thawing are conducted repeatedly for five times, centrifuging is conducted after the last time of thawing, supernatant is taken and put into a dialysis bag, the supernatant is dialyzed overnight under the environment of 0 DEG C to 4 DEG C through pyrogen-free cold normal saline, dialysate outside the bag is collected and is subjected to filtration sterilization through a filtering film, and the dialysate is subjected to sterile subpackaging and is then subjected to freezing preservation. After the biological immune preparation enters an organism, the effect of the biological immune preparation can be rapidly developed and can last for several months or even longer, the immune antibody level of the vaccine is effectively improved, the biological immune preparation can be rapidly and completely absorbed by the organism without any residue, weight increasing is obvious after the biological immune preparation is injected into the organism, and a goat can be lively in spirit.

The invention relates to a preparation method for hemoglobin. The method is characterized by comprising the following steps: taking a proper amount of human blood, adding normal saline (a 0.9% NaCl solution) with the volume being 3-6 times that of the human blood, and performing centrifugation at a speed of 2,800 rpm and a temperature of 4 DEG C for 15-30 minutes; obtaining supernatant liquid containing leukocytes, plasma proteins and platelets, extruding the supernatant liquid, remaining lower-layer packed red cells, repeating a washing process for 3-5 times until the supernatant liquid is basically colorless, removing other substances except the red cells to the maximum extent, and obtaining clean packed red cells; taking a certain amount of the packed red cells, adding sterilization and high-purity de-ionized water with the same volume for pre-expansion for 5-15 minutes, finally adding water with the volume being 2 times that of a pre-expansion solution for hyposmosis for 10-20 minutes, and performing centrifugation at a speed of 10,000 rpm for 30-60 minutes, wherein the centrifuged supernatant liquid is a hemoglobin solution.

The invention relates to a nimodipine fat emulsion concentrate and a preparation method and use thereof, and belongs to the field of medicine and pharmacy. The nimodipine fat emulsion concentrate overcomes the defects of complex traditional fat milk preparation process and poor product stability. The nimodipine fat emulsion concentrate is simple in preparation process only needing simple physical mixing without homogenization process. The product can be sterilized through a 0.22 mum millipore filter film, and can spontaneously emulsify during clinical use by dilution with normal saline or glucose solution and other water solutions and slight oscillation, and under the optimal conditions, average particle size is about 0.2 mum, and fully shows injection fat milk properties. The nimodipine fat emulsion concentrate product has good liquidity, and may not be hung on the wall, the appearance is single-phase, transparent and clear, clarity detection is acceptable, and after repeated freezing and thawing, preparation stratification phenomenon does not occur. The nimodipine fat emulsion concentrate product is used for prevention and treatment of ischemic neurological damage caused by cerebral angiospasm after subarachnoid hemorrhage and senile brain function impairment, hemicranias, sudden deafness and other diseases.

The invention discloses a method for constructing an azoospermia mouse model, which comprises the following steps: (1) providing a sexually mature male mouse with a body weight of 25-35 g; (2) dissolving busulfan in DMSO and diluting it with normal saline (3) Alcohol disinfection of the lower abdomen of the mouse, after drying, draw the diluted solution obtained in step (2) and insert the needle, and after withdrawing to confirm that it is correct, complete the first intraperitoneal injection; after that, inject 3 times at intervals within the same day, After 35-37 days, the mouse model of azoospermia was obtained. The method of the present invention has high molding rate and good stability; according to the present invention, 84 C57BL / 6 mice have been accumulatively completed modeling of azoospermia, and the total number of moldings identified by testis HE staining is 84 / 84, and the molding rate is 84 / 84. 100%; adopt the method of the present invention to build the model, the mortality rate of the mice is 0%; according to the method of the present invention, the total number of deaths of 84 C57BL / 6 mice that have been injected is 0, and the mortality rate is 0%.

The invention discloses a biological degreasing agent and a preparation method thereof. The method comprises the steps of inoculating lactobacillus into a liquid medium for culturing, and then diluting by using normal saline; sequentially adding Na2CO3 and hydrochloric acid; and carrying out standing and centrifuging and collecting supernate to obtain the biological degreasing agent. According to the prepared biological degreasing agent, greasy dirt on tableware can be effectively removed within an extremely short period of time; and the oil washing rate reaches 83.3-92.3%. Compared with the existing degreasing agent, the biological degreasing agent has the advantages of being mild in property, environment-friendly, nontoxic, short in degreasing time and high in efficiency.

The invention relates to a method for preparing a human anatomical visceral specimen. The method comprises the following steps: step one, pouring normal saline into a viscus through an artery for washing and cleaning; step two, pouring visceral and immersing the visceral artery into pretreatment liquid for 12 to 24 hours, and then carrying out vacuum treatment; step three, carrying out pre coolingon the viscus for 3 to 5h at a temperature of 2 DEG C, and immersing the cooled viscus into a dehydration solution for 16 to 24h; step four, immersing the viscus into a sealed container with preservation solution for 36 to 72h and carrying out gradient vacuum treatment; step five, removing excess material of the visceral outer layer to obtain a softened specimen; and step six, placing the softened specimen into a sealed box filled with a curing agent for 72 to 96 hours to obtain a cured specimen. The method is simple and easy. Since the pretreatment liquid and the preservation liquid are notirritating, the operator is protected from being injured; the softened visceral specimen is not easy to damage and can be used for long time; and the visceral specimen has the natural color and soft texture and has no mildew phenomenon.

The invention discloses wild enterococcus faecium ZK03 as well as an electric shock transformation method and application thereof, and belongs to the field of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center on January 5, 2022, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No. 62192. The strain does not contain enterococcus virulence factors such as Asa1, cylA, efaA, esp, gelE and hyl. The strain has the characteristics of good acid resistance, swine bile salt resistance, high temperature resistance and normal saline resistance, and can survive for a long time in normal saline at room temperature; the strain has a certain inhibition effect on staphylococcus aureus, salmonella and escherichia coli, can be used for preparing a bacteriostatic agent, and can also be used as a feed additive for fermentation production of feed. The electric shock transformation method of the wild enterococcus faecium ZK03 is simple to operate, high in transformation efficiency and strong in repeatability.