Biological degreasing agent and preparation method thereof
A technology for biological degreasing and degreasing agent, applied in the field of cleaning agents, can solve the problems of poor degreasing ability, difficult cleaning, waste of water resources, etc., and achieve the effects of short degreasing time, mild nature and high efficiency
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Embodiment 1
[0018] 1. Preparation of biological degreasing agent
[0019] Inoculate Lactobacillus sp. CICC10481 (purchased from China Industrial Microorganism Culture Collection Management Center, the preservation number is CICC10481) into 1L of liquid medium, then place it in a culture shaker at 30°C, and shake it at 165r / min After 14 hours, the bacterial suspension was obtained, and the concentration of the bacterial suspension was adjusted to OD with physiological saline 590 is 0.2; add 300 μL Na with a concentration of 1mol / L 2 CO 3 solution, put it in an incubator at 30°C for 30 minutes, then add 200 μL of dilute hydrochloric acid solution with a concentration of 2.48mol / L, let it stand for 30 minutes at room temperature, centrifuge at 8000r / min, and collect the supernatant into a 1L sample collection bottle , to obtain biological degreaser.
[0020] Wherein, the formula of liquid culture medium is that every liter of culture medium contains: inulin 5g, ZnCl 2 0.05g, CaCl 2 0....
Embodiment 2
[0030] 1. Preparation of biological degreasing agent
[0031] Inoculate Lactobacillus sp. CICC10481 (purchased from China Industrial Microorganism Culture Collection and Management Center, the preservation number is CICC10481) into 1L of liquid medium, then place it in a culture shaker at 37°C, and shake it at 165r / min After 16 hours, the bacterial suspension was obtained, and the concentration of the bacterial suspension was adjusted to OD with physiological saline 590 is 0.3; add 500 μL of Na with a concentration of 1mol / L 2 CO 3 solution, put it in an incubator at 37°C for 30min, then add 300μL of dilute hydrochloric acid solution with a concentration of 2.48mol / L, let it stand for 30min at room temperature, centrifuge at 8000r / min, and collect the supernatant into a 1L sample collection bottle , to obtain biological degreaser.
[0032] Wherein, the formula of liquid culture medium is that every liter of culture medium contains: inulin 5g, ZnCl 2 0.05g, CaCl 2 0.02g,...
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