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Preparation method for hemoglobin

A technology of hemoglobin and human blood, applied in the field of biomedicine, can solve problems such as organ damage, vasoconstriction, and blood pressure rise

Inactive Publication Date: 2015-07-29
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these products are used in clinical practice, there are toxic and side effects such as vasoconstriction, increased blood pressure, and damage to the heart, kidney, liver and other organs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0011] Embodiment 2 pre-expansion

[0012] Take a certain amount of packed red blood cells and add an equal volume of sterilized high-purity deionized water for pre-expansion. At 24, 26, 28, and 30 minutes, observe the cell morphology at different pre-swelling times under a 400-fold microscope, and determine the best pre-swelling time according to the roundness of the cells.

Embodiment 3

[0013] Embodiment 3 hypotonic membrane rupture

[0014] Add 2 times the volume of water to the pre-swollen red blood cells to rupture the membrane, and the time is set at 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 , 48, and 50 minutes, observe the cell morphology at different membrane rupture times under a 400-fold microscope, and determine the optimal membrane rupture time according to the degree of cell rupture, the concentration of purified hemoglobin, and the difficulty of filtering the membrane rupture solution. Then centrifuge at 10,000 rpm for 30-60 minutes, and the supernatant after centrifugation is a hemoglobin solution.

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PUM

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Abstract

The invention relates to a preparation method for hemoglobin. The method is characterized by comprising the following steps: taking a proper amount of human blood, adding normal saline (a 0.9% NaCl solution) with the volume being 3-6 times that of the human blood, and performing centrifugation at a speed of 2,800 rpm and a temperature of 4 DEG C for 15-30 minutes; obtaining supernatant liquid containing leukocytes, plasma proteins and platelets, extruding the supernatant liquid, remaining lower-layer packed red cells, repeating a washing process for 3-5 times until the supernatant liquid is basically colorless, removing other substances except the red cells to the maximum extent, and obtaining clean packed red cells; taking a certain amount of the packed red cells, adding sterilization and high-purity de-ionized water with the same volume for pre-expansion for 5-15 minutes, finally adding water with the volume being 2 times that of a pre-expansion solution for hyposmosis for 10-20 minutes, and performing centrifugation at a speed of 10,000 rpm for 30-60 minutes, wherein the centrifuged supernatant liquid is a hemoglobin solution.

Description

(1) Technical field: [0001] The invention relates to the field of biomedicine, in particular to a preparation method of hemoglobin. (two) background technology: [0002] The traditional blood transfusion method with transfusion of whole blood as the main means has played an extremely important role in medical rescue, but at the same time, it also has various shortcomings that are difficult to overcome. Risk of viral cross-infection, strained blood sources, short storage period and special facilities are required. In order to solve the above contradictions, people started research on red blood cell substitutes as early as the end of the 19th century. Red blood cell substitute refers to the general term for various oxygen carriers that can expand blood volume, maintain the balance of osmotic pressure inside and outside blood vessels, and have the function of carrying oxygen to replace human red blood cells. The development of blood substitutes has gone through the initial va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/805C07K1/14
CPCC07K14/805
Inventor 李凤娟张释元刘洋候依萍黄雪雪蒋坤
Owner TIANJIN POLYTECHNIC UNIV
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