Establishment of animal model of non-compressive lumbar disc nucleus pulposus herniation

Abstract

The present invention discloses an establishment method of an animal model of non-compressive lumbar disc nucleus pulposus herniation. The method comprises the following steps: S1, cutting a rat tail1 cm from a root part of the rat tail and suturing wound to obtain rat caudal vertebra nucleus pulposus; S2, incising fibrous rings of the rat caudal vertebra nucleus pulposus, the nucleus pulposus isjelly-like, and taking 5 nucleus pulposus and 50 [mu]l normal saline to be fully mixed and diluted into a suspension; and S3, injecting 20 [mu]l of the nucleus pulposus suspension and 30 [mu]l of 2%of a lidocaine injection to epidural spaces of lumbar vertebra to enable the suspension to fill a periphery of nerve roots. Characteristics that the rat tail is thick and large and contains enough intervertebral disc tissue, and ithe nucleus pulposus is jelly-like, an epidural puncture technique is combined, the rat autologous nucleus pulposus suspension is injected into the epidural spaces of thelumbar vertebra to produce nucleus pulposus tissues which are exposed to a blood circulation and body immune system. The establishment method confirms that even if the nucleus pulposus suspension inlumbar vertebra canal of the rat does not compress the nerve roots, changes in nerve root functions and morphology also appear, and proves effectiveness of the model.

Description

Technical field [0001] The invention belongs to the technical field of animal model establishment of animal medicine, and particularly relates to the establishment of a non-compression type lumbar intervertebral disc nucleus pulposus animal model. Background technique [0002] The traditional view is that the protruding lumbar disc nucleus pulposus compresses the nerve roots as the cause of sciatica. In recent years, with the deepening of people's understanding of lumbar disc herniation (LDH), it has been found that even if the nerve root is not compressed by the protruding nucleus pulposus, sciatica will occur, and its pathogenesis is not clear. People hope to establish a reliable and effective non-compression animal model of lumbar disc nucleus pulposus, and by applying intervention factors, accurately observe the changes produced in the model, explore the mechanism of disease, and extend the research results to the clinic for treatment Lumbar disc herniation provides reasonab...

AI Technical Summary

Problems solved by technology

The shortcomings of this model are: 1. The nucleus pulposus causes compression on the nerve root

2. The operation trauma is large, the mortality rate of rats is high, and the operation itself may damage the nerves

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