Wild enterococcus faecium ZK03 and electric shock transformation method and application thereof

A technology of Enterococcus faecium and ZK03, applied in the field of microorganisms, can solve the problems of difficulty in obtaining positive transformation, low transformation efficiency, and improving transformation difficulty.
CN114480211APending Publication Date: 2022-05-13ZHONGKAI UNIV OF AGRI & ENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHONGKAI UNIV OF AGRI & ENG
Publication Date
2022-05-13

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Abstract

The invention discloses wild enterococcus faecium ZK03 as well as an electric shock transformation method and application thereof, and belongs to the field of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center on January 5, 2022, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No. 62192. The strain does not contain enterococcus virulence factors such as Asa1, cylA, efaA, esp, gelE and hyl. The strain has the characteristics of good acid resistance, swine bile salt resistance, high temperature resistance and normal saline resistance, and can survive for a long time in normal saline at room temperature; the strain has a certain inhibition effect on staphylococcus aureus, salmonella and escherichia coli, can be used for preparing a bacteriostatic agent, and can also be used as a feed additive for fermentation production of feed. The electric shock transformation method of the wild enterococcus faecium ZK03 is simple to operate, high in transformation efficiency and strong in repeatability.
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Description

technical field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of wild-type Enterococcus faecium ZK03 and its electric shock transformation method and application. Background technique

[0002] Enterococcus Faecium (E. faecium) is one of the normal intestinal flora of humans and animals. It belongs to Gram-positive cocci without capsule and flagella. It has good acid resistance, high temperature resistance and bile salt resistance. According to a large number of literature reports, Enterococcus faecium can produce lactic acid, bacteriocin, hydrogen peroxide and other substances, and has a certain inhibitory effect on Escherichia coli, Salmonella, Staphylococcus aureus, etc. In food, the fermentation of Enterococcus faecium contributes to the development of aroma and flavor, prolongs shelf life, and improves the microbial environment. In animal husbandry, Enterococcus faecium is often used as one of the tecan prod...

Claims

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